Construct: ORF ccsbBroadEn_07727

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017403.1_s300c1, BRDN0000397502
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LIAS (11019)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11019 LIAS lipoic acid synthetase NM_006859.4 99.9% 99.7% 758C>T
2 human 11019 LIAS lipoic acid synthetase NM_001278590.2 88.3% 88.1% 606_607ins129;629C>T
3 human 11019 LIAS lipoic acid synthetase NM_194451.3 86.2% 85.4% (many diffs)
4 human 11019 LIAS lipoic acid synthetase NM_001363700.2 72.2% 67% 218_219ins94;298_299ins215;449C>T
5 human 11019 LIAS lipoic acid synthetase NM_001278591.1 37.1% 36% (many diffs)
6 human 11019 LIAS lipoic acid synthetase NM_001278592.1 26.7% 19.3% 218_219ins94;300_300delGins723
7 mouse 79464 Lias lipoic acid synthetase NM_024471.5 86.4% 89.5% (many diffs)
8 mouse 79464 Lias lipoic acid synthetase NM_001310612.1 66.8% 70.8% (many diffs)
9 mouse 79464 Lias lipoic acid synthetase XM_006504195.2 23.2% 17% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1182
ORF length:
1116
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTC TCTACGCTGC GGGGATGCAG CCCGCACCCT GGGGCCCCGG GTATTTGGGA
121GATATTTTTG CAGCCCAGTC AGACCGTTAA GCTCCTTGCC AGATAAAAAA AAGGAACTCC
181TACAGAATGG ACCAGACCTT CAAGATTTTG TATCTGGTGA TCTTGCAGAC AGGAGCACCT
241GGGATGAATA TAAAGGAAAC CTAAAACGCC AGAAAGGAGA AAGGTTAAGA CTACCTCCAT
301GGCTAAAGAC AGAGATTCCC ATGGGGAAAA ATTACAATAA ACTGAAAAAT ACTTTGCGGA
361ATTTAAATCT CCATACAGTA TGTGAGGAAG CTCGATGTCC CAATATTGGA GAGTGTTGGG
421GAGGTGGAGA ATATGCCACC GCCACAGCCA CGATCATGTT GATGGGTGAC ACATGTACAA
481GAGGTTGCAG ATTTTGTTCT GTTAAGACTG CAAGAAATCC TCCTCCACTG GATGCCAGTG
541AGCCCTACAA TACTGCAAAG GCAATTGCAG AATGGGGTCT GGATTATGTT GTCCTGACAT
601CTGTGGATCG AGATGATATG CCTGATGGGG GAGCTGAACA CATTGCAAAG ACCGTATCAT
661ATTTAAAGGA AAGGAATCCA AAAATCCTTG TGGAGTGTCT TACTCCTGAT TTTCGAGGTG
721ATCTCAAAGC AATAGAAAAA GTTGCTCTGT CAGGATTAGA TGTGTATGCA CATAATGTAG
781AAACAGTCCC GGAATTACAG AGTAAGGTTC GTGATCCTCG GGTCAATTTT GATCAGTCCC
841TACGTGTACT GAAACATGCC AAGAAGGTTC AGCCTGATGT TATTTCTAAA ACATCTATAA
901TGTTGGGTTT AGGCGAGAAT GATGAGCAAG TATATGCAAC AATGAAAGCA CTTCGTGAGG
961CAGATGTAGA CTGCTTGACT TTAGGACAAT ATATGCAGCC AACAAGGCGT CACCTTAAGG
1021TTGAAGAATA TATTACTCCT GAAAAATTCA AATACTGGGA AAAAGTAGGA AATGAACTTG
1081GATTTCATTA TACTGCAAGT GGCCCTTTGG TGCGTTCTTC ATATAAAGCA GGTGAATTTT
1141TCCTGAAAAA TCTAGTGGCT AAAAGAAAAA CAAAAGACCT CTACCCAACT TTCTTGTACA
1201AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac

Download FASTA (ORF) (Full)

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