Construct: ORF ccsbBroadEn_07972

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006743.1_s300c1, BRDN0000396190
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHMP2B (25978)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25978 CHMP2B charged multivesicular body... NM_014043.4 99.8% 100% 312T>C
2 human 25978 CHMP2B charged multivesicular body... XM_011533576.2 91.8% 88.6% (many diffs)
3 human 25978 CHMP2B charged multivesicular body... NM_001244644.2 80.4% 80.2% 1_1delAins124;189T>C
4 mouse 68942 Chmp2b charged multivesicular body... NM_026879.2 89.6% 99.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
705
ORF length:
639
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GTCCCTCTTC AAGAAGAAAA CCGTGGATGA TGTAATAAAG GAACAGAATC
121GAGAGTTACG AGGTACACAG AGGGCTATAA TCAGAGATCG AGCAGCTTTA GAGAAACAAG
181AAAAACAGCT GGAATTAGAA ATTAAGAAAA TGGCCAAGAT TGGTAATAAG GAAGCTTGCA
241AAGTTTTAGC CAAACAACTT GTGCATCTAC GGAAACAGAA GACGAGAACT TTTGCTGTAA
301GTTCAAAAGT TACTTCTATG TCTACACAAA CAAAAGTGAT GAATTCCCAA ATGAAGATGG
361CTGGAGCAAT GTCTACCACA GCAAAAACAA TGCAGGCAGT TAACAAGAAG ATGGATCCAC
421AAAAGACATT ACAAACAATG CAGAATTTCC AGAAGGAAAA CATGAAAATG GAAATGACTG
481AAGAAATGAT CAATGATACA CTTGATGACA TCTTTGACGG TTCTGATGAC GAAGAAGAAA
541GCCAGGATAT TGTGAATCAA GTTCTTGATG AAATTGGAAT TGAAATTTCT GGAAAGATGG
601CCAAAGCTCC ATCAGCTGCT CGAAGCTTAC CATCTGCCTC TACTTCAAAG GCTACAATCT
661CAGATGAAGA GATTGAACGG CAACTCAAGG CTTTAGGAGT AGATTACCCA ACTTTCTTGT
721ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)