Construct: ORF ccsbBroadEn_08100
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017303.1_s300c1, BRDN0000389553
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLF15 (28999)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28999 | KLF15 | Kruppel like factor 15 | NM_014079.4 | 99.9% | 100% | 1242C>A |
| 2 | human | 28999 | KLF15 | Kruppel like factor 15 | XM_005247400.4 | 99.9% | 100% | 1242C>A |
| 3 | human | 28999 | KLF15 | Kruppel like factor 15 | XM_011512743.2 | 95.5% | 95.6% | 1_57del;1299C>A |
| 4 | human | 28999 | KLF15 | Kruppel like factor 15 | XR_924449.2 | 26.4% | (many diffs) | |
| 5 | human | 28999 | KLF15 | Kruppel like factor 15 | XR_001740124.2 | 24.3% | (many diffs) | |
| 6 | human | 28999 | KLF15 | Kruppel like factor 15 | XR_002959509.1 | 22.6% | (many diffs) | |
| 7 | mouse | 66277 | Klf15 | Kruppel-like factor 15 | NM_023184.3 | 83.3% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1314
- ORF length:
- 1248
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGT GGACCACTTA CTTCCAGTGG ACGAGAACTT CTCGTCGCCA AAATGCCCAG 121 TTGGGTATCT GGGTGATAGG CTGGTTGGCC GGCGGGCATA TCACATGCTG CCCTCACCCG 181 TCTCTGAAGA TGACAGCGAT GCCTCCAGCC CCTGCTCCTG TTCCAGTCCC GACTCTCAAG 241 CCCTCTGCTC CTGCTATGGT GGAGGCCTGG GCACCGAGAG CCAGGACAGC ATCTTGGACT 301 TCCTATTGTC CCAGGCCACG CTGGGCAGTG GCGGGGGCAG CGGCAGTAGC ATTGGGGCCA 361 GCAGTGGCCC CGTGGCCTGG GGGCCCTGGC GAAGGGCAGC GGCCCCTGTG AAGGGGGAGC 421 ATTTCTGCTT GCCCGAGTTT CCTTTGGGTG ATCCTGATGA CGTCCCACGG CCCTTCCAGC 481 CTACCCTGGA GGAGATTGAA GAGTTTCTGG AGGAGAACAT GGAGCCTGGA GTCAAGGAGG 541 TCCCTGAGGG CAACAGCAAG GACTTGGATG CCTGCAGCCA GCTCTCAGCT GGGCCACACA 601 AGAGCCACCT CCATCCTGGG TCCAGCGGGA GAGAGCGCTG TTCCCCTCCA CCAGGTGGTG 661 CCAGTGCAGG AGGTGCCCAG GGCCCAGGTG GGGGCCCCAC GCCTGATGGC CCCATCCCAG 721 TGTTGCTGCA GATCCAGCCC GTGCCTGTGA AGCAGGAATC GGGCACAGGG CCTGCCTCCC 781 CTGGGCAAGC CCCAGAGAAT GTCAAGGTTG CCCAGCTCCT GGTCAACATC CAGGGGCAGA 841 CCTTCGCACT CGTGCCCCAG GTGGTACCCT CCTCCAACTT GAACCTGCCC TCCAAGTTTG 901 TGCGCATTGC CCCTGTGCCC ATTGCCGCCA AGCCTGTTGG ATCGGGACCC CTGGGGCCTG 961 GCCCTGCCGG TCTCCTCATG GGCCAGAAGT TCCCCAAGAA CCCAGCCGCA GAACTCATCA 1021 AAATGCACAA ATGTACTTTC CCTGGCTGCA GCAAGATGTA CACCAAAAGC AGCCACCTCA 1081 AGGCCCACCT GCGCCGGCAC ACGGGTGAGA AGCCCTTCGC CTGCACCTGG CCAGGCTGCG 1141 GCTGGAGGTT CTCGCGCTCT GACGAGCTGT CGCGGCACAG GCGCTCGCAC TCAGGTGTGA 1201 AGCCGTACCA GTGTCCTGTG TGCGAGAAGA AGTTCGCGCG GAGCGACCAC CTCTCCAAGC 1261 ACATCAAGGT GCACCGCTTC CCGCGGAGCA GCCGCTCCGT GCGCTCAGTG AACTGCCCAA 1321 CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac