Construct: ORF ccsbBroadEn_08129
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014691.1_s300c1, BRDN0000395742
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANAPC2 (29882)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29882 | ANAPC2 | anaphase promoting complex ... | NM_013366.4 | 99.9% | 99.7% | 794T>C;2456A>C |
| 2 | human | 29882 | ANAPC2 | anaphase promoting complex ... | XR_929780.2 | 90.4% | (many diffs) | |
| 3 | human | 29882 | ANAPC2 | anaphase promoting complex ... | XR_929779.2 | 90.2% | (many diffs) | |
| 4 | human | 29882 | ANAPC2 | anaphase promoting complex ... | XR_929781.2 | 88.8% | (many diffs) | |
| 5 | mouse | 99152 | Anapc2 | anaphase promoting complex ... | NM_175300.4 | 84.6% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 2533
- ORF length:
- 2466
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAA 61 GTTGGCATGG CGGCGGCAGT TGTGGTGGCG GAGGGGGACA GCGACTCCCG GCCCGGACAG 121 GAGTTGTTAG TGGCCTGGAA CACCGTGAGC ACCGGCCTGG TGCCGCCGGC TGCGCTGGGG 181 CTGGTGTCTT CCCGGACCAG CGGTGCAGTC CCGCCAAAGG AAGAGGAGCT CCGGGCGGCG 241 GTGGAGGTTC TGAGGGGCCA CGGGCTACAC TCGGTCCTGG AGGAGTGGTT CGTGGAGGTG 301 CTGCAGAACG ATCTGCAGGC CAACATCTCC CCTGAGTTCT GGAATGCCAT CTCCCAATGC 361 GAGAACTCTG CGGATGAGCC CCAGTGCCTT TTGCTACTCC TTGACGCTTT TGGCCTGCTG 421 GAGAGCCGCC TGGATCCCTA CCTGCGTAGC CTAGAGCTGC TGGAGAAATG GACTCGCCTG 481 GGCTTGCTGA TGGGCACTGG TGCTCAGGGG CTGCGAGAAG AAGTCCACAC TATGTTGCGC 541 GGAGTCTTGT TCTTTAGCAC CCCCAGAACC TTCCAAGAGA TGATCCAGCG TCTGTATGGG 601 TGCTTCTTGA GAGTCTATAT GCAGAGTAAG AGGAAGGGGG AAGGGGGCAC AGACCCGGAA 661 CTGGAAGGGG AGCTGGACAG CCGGTATGCC CGTCGCCGGT ACTACCGGCT CCTGCAGAGC 721 CCGCTGTGTG CAGGGTGCAG CAGTGACAAG CAACAGTGCT GGTGTCGCCA GGCTCTGGAG 781 CAGTTCCATC AGCTCAGCCA GGTCTTACAC AGGCTCAGTC TGCTGGAGCG GGTCAGTGCC 841 GAGGCTGTGA CCACCACCCC GCACCAGGTG ACCCGGGAGA GGATGGAGGA CCGTTGCCGG 901 GGCGAGTACG AGCGCTCCTT CCTGCGTGAG TTCCACAAGT GGATCGAGCG GGTGGTCGGC 961 TGGCTCGGCA AGGTGTTCCT GCAGGACGGC CCCGCCAGGC CCGCATCTCC CGAGGCCGGC 1021 AACACCCTGC GCCGCTGGCG CTGCCACGTG CAAAGGTTCT TCTACCGCAT CTACGCCAGC 1081 CTGCGCATCG AGGAGCTCTT CAGCATCGTC CGAGACTTCC CAGACTCCCG GCCAGCCATC 1141 GAGGACCTCA AGTACTGCCT GGAGAGGACG GACCAGAGGC AGCAGCTGCT CGTGTCCCTC 1201 AAGGCTGCCC TGGAGACTCG GCTCCTGCAT CCAGGCGTCA ACACGTGTGA CATCATCACC 1261 CTCTATATCT CTGCCATCAA GGCGCTGCGC GTGCTGGACC CTTCCATGGT CATCCTGGAG 1321 GTGGCCTGTG AGCCTATCCG CCGCTACCTG AGGACGCGGG AGGACACAGT GCGGCAGATT 1381 GTGGCTGGGC TGACGGGGGA CTCGGACGGG ACAGGGGACC TGGCTGTTGA GCTGTCCAAG 1441 ACCGACCCGG CGAGCCTGGA GACAGGCCAG GACAGTGAGG ATGACTCAGG CGAGCCAGAG 1501 GACTGGGTCC CGGACCCTGT GGATGCCGAT CCAGGGAAGT CGAGCTCCAA GCGGCGTTCA 1561 TCGGACATCA TCAGCCTGCT GGTCAGCATC TACGGCAGCA AGGACCTCTT CATCAATGAG 1621 TACCGCTCGC TGCTGGCCGA CCGCCTGCTG CACCAGTTCA GCTTCAGCCC CGAGCGGGAG 1681 ATCCGCAACG TGGAGCTGCT GAAGCTGCGC TTTGGCGAGG CCCCAATGCA CTTCTGTGAA 1741 GTCATGCTGA AGGACATGGC GGACTCCCGC CGCATCAATG CCAACATCCG GGAGGAGGAT 1801 GAGAAGCGGC CAGCAGAGGA GCAGCCACCG TTCGGGGTCT ACGCTGTCAT CCTGTCCAGT 1861 GAGTTCTGGC CGCCCTTCAA GGACGAGAAG CTGGAGGTCC CCGAGGATAT CAGGGCAGCC 1921 CTGGAGGCTT ACTGCAAGAA GTATGAGCAG CTCAAGGCCA TGCGGACCCT CAGTTGGAAG 1981 CACACCCTGG GCCTGGTGAC CATGGACGTG GAGCTGGCCG ACCGCACGCT GTCTGTGGCG 2041 GTCACCCCAG TACAGGCGGT GATCTTGCTG TATTTTCAGG ACCAAGCCAG CTGGACCCTG 2101 GAGGAACTGA GCAAGGCGGT GAAGATGCCC GTGGCGCTGC TGCGGCGGCG GATGTCCGTG 2161 TGGCTGCAGC AGGGTGTGCT GCGTGAGGAG CCCCCCGGCA CCTTCTCTGT CATTGAGGAG 2221 GAGCGGCCTC AGGACCGGGA CAACATGGTG CTCATTGACA GTGACGACGA GAGCGACTCC 2281 GGCATGGCCT CCCAGGCCGA CCAGAAGGAG GAGGAGCTGC TGCTCTTCTG GACGTACATC 2341 CAGGCCATGC TGACCAACCT GGAGAGCCTC TCACTGGATC GTATCTACAA CATGCTCCGC 2401 ATGTTTGTGG TGACTGGGCC TGCACTGGCC GAGATTGACC TGCAGGAGCT GCAGGGCTAC 2461 CTGCAGAAGA AGGTGCGGGA CCAGCAGCTC GTCTACTCGG CCGGCGTCTA CCGCCTGCCC 2521 ACGAACTGCA GCTGCCCAAC TTTGTTGTAC AAAGTtggca ttataagaaa gcattgctta 2581 tcaatttgtt gcaacgaac