Construct: ORF ccsbBroadEn_08137

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF006232.1_s300c1, BRDN0000395434
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: HOOK2 (29911)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	29911	HOOK2	hook microtubule tethering ...	NM_001100176.2	99.9%	99.8%	1464C>G
2	human	29911	HOOK2	hook microtubule tethering ...	XM_005259874.2	99.8%	99.7%	1464C>G;1717_1718insCAG
3	human	29911	HOOK2	hook microtubule tethering ...	NM_013312.3	99.6%	99.5%	1464C>G;1599_1604delTGCCAT
4	human	29911	HOOK2	hook microtubule tethering ...	XM_005259873.2	99.5%	99.4%	1464C>G;1599_1604delTGCCAT;1723_1724insCAG
5	human	29911	HOOK2	hook microtubule tethering ...	XM_017026721.1	89.7%	89.6%	0_1ins219;1245C>G
6	human	29911	HOOK2	hook microtubule tethering ...	XM_005259876.4	89.5%	89.4%	0_1ins219;1245C>G;1380_1385delTGCCAT
7	human	29911	HOOK2	hook microtubule tethering ...	XM_005259877.4	89.5%	89.4%	0_1ins219;1245C>G;1380_1385delTGCCAT
8	human	29911	HOOK2	hook microtubule tethering ...	XM_011527943.2	89.5%	89.4%	0_1ins219;1245C>G;1380_1385delTGCCAT
9	human	29911	HOOK2	hook microtubule tethering ...	XM_011527944.2	89.5%	89.4%	0_1ins219;1245C>G;1380_1385delTGCCAT
10	human	29911	HOOK2	hook microtubule tethering ...	XM_011527945.2	89.5%	89.4%	0_1ins219;1245C>G;1380_1385delTGCCAT
11	human	29911	HOOK2	hook microtubule tethering ...	XM_011527946.2	89.5%	89.4%	0_1ins219;1245C>G;1380_1385delTGCCAT
12	human	29911	HOOK2	hook microtubule tethering ...	XM_017026720.1	89.5%	89.4%	0_1ins219;1245C>G;1380_1385delTGCCAT
13	human	29911	HOOK2	hook microtubule tethering ...	XM_011527947.2	80.8%	80.8%	0_1ins405;1059C>G;1194_1199delTGCCAT
14	mouse	170833	Hook2	hook microtubule tethering ...	NM_133255.2	83.4%	86%	(many diffs)
15	mouse	170833	Hook2	hook microtubule tethering ...	NM_001167991.1	83.2%	85.9%	(many diffs)
16	mouse	170833	Hook2	hook microtubule tethering ...	XM_011248323.2	83.1%	85.6%	(many diffs)
17	mouse	170833	Hook2	hook microtubule tethering ...	XM_017312592.1	83%	85.6%	(many diffs)
18	mouse	170833	Hook2	hook microtubule tethering ...	XM_006530740.3	81.9%	84.4%	(many diffs)
19	mouse	170833	Hook2	hook microtubule tethering ...	XM_006530741.3	81.7%	84.2%	(many diffs)
20	mouse	170833	Hook2	hook microtubule tethering ...	XM_006530739.3	81.7%	84.1%	(many diffs)
21	mouse	170833	Hook2	hook microtubule tethering ...	XM_006530738.3	81.6%	84%	(many diffs)
22	mouse	170833	Hook2	hook microtubule tethering ...	XM_017312591.1	81.5%	84%	(many diffs)
23	mouse	170833	Hook2	hook microtubule tethering ...	XM_006530736.3	76.5%	78.7%	(many diffs)
24	mouse	170833	Hook2	hook microtubule tethering ...	XM_006530737.3	76.4%	78.6%	(many diffs)
25	mouse	170833	Hook2	hook microtubule tethering ...	XM_006530742.2	67.3%	70.4%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

2217

ORF length:

2151

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61	TTGGCATGAG CGTGGACAAA GCTGAGCTAT GCGGGTCTCT GCTCACCTGG TTACAGACGT
121	TCCACGTTCC GTCTCCCTGT GCCAGCCCTC AGGACCTGAG CAGCGGCCTT GCCGTAGCCT
181	ATGTGCTGAA CCAGATAGAC CCCTCCTGGT TCAACGAGGC ATGGCTCCAG GGCATCTCGG
241	AAGATCCAGG TCCCAACTGG AAGCTGAAGG TCAGCAATCT GAAGATGGTC TTACGGAGCC
301	TAGTAGAGTA CTCCCAGGAT GTCCTGGCGC ATCCTGTGTC AGAAGAGCAT CTCCCAGATG
361	TGAGCCTCAT TGGAGAGTTC TCAGACCCGG CAGAGCTCGG CAAGCTGCTT CAGCTGGTGC
421	TGGGCTGTGC CATCAGTTGC GAGAAAAAGC AGGACCACAT CCAGAGAATC ATGACGCTGG
481	AAGAATCGGT TCAGCATGTG GTGATGGAAG CCATCCAAGA GCTCATGACC AAAGACACTC
541	CTGACTCCCT GTCACCAGAG ACGTATGGCA ACTTTGACAG CCAGTCCCGC AGGTACTATT
601	TCCTAAGTGA GGAGGCTGAG GAGGGGGACG AATTACAGCA GCGCTGTCTG GATCTGGAGC
661	GGCAGCTGAT GCTCCTGTCA GAGGAGAAGC AGAGCCTGGC GCAAGAGAAT GCAGGGCTGC
721	GGGAGCGGAT GGGCCGGCCT GAAGGCGAGG GTACCCCAGG TCTCACTGCC AAGAAGCTGC
781	TGCTGCTGCA ATCCCAGCTG GAGCAGTTGC AGGAGGAGAA CTTCAGGCTG GAGAGTGGCA
841	GGGAGGATGA GCGCCTGCGC TGTGCCGAGC TGGAGAGGGA GGTTGCGGAG CTGCAGCACC
901	GGAACCAGGC GCTGACTAGC CTGGCCCAGG AGGCACAGGC CCTGAAGGAT GAGATGGATG
961	AACTACGGCA GTCTTCGGAG CGTGCTGGGC AGCTGGAGGC CACGCTGACC AGTTGCCGGC
1021	GCCGCTTGGG CGAGCTGAGG GAGCTGCGGC GGCAGGTGCG GCAGCTGGAG GAACGCAACG
1081	CCGGCCACGC CGAGCGCACG CGACAACTGG AGGATGAGCT ACGCCGAGCG GGCTCCCTGC
1141	GCGCCCAGCT GGAGGCGCAG CGGCGGCAGG TGCAGGAACT GCAGGGCCAG CGGCAGGAGG
1201	AGGCCATGAA GGCCGAGAAA TGGCTATTTG AATGCCGCAA CCTGGAGGAA AAGTATGAGT
1261	CGGTGACAAA GGAGAAGGAG CGGCTGTTGG CGGAGCGGGA CTCCTTGCGG GAGGCCAATG
1321	AGGAGCTGCG CTGCGCCCAG CTGCAGCCGC GGGGGTTGAC CCAGGCCGAT CCCTCACTGG
1381	ATCCCACCTC CACACCCGTG GATAACTTAG CCGCAGAGAT CCTGCCTGCG GAGCTCAGGG
1441	AGACGCTCCT GCGGCTTCAG CTGGAGAACA AGCGGCTGTG CAGGCAGGAG GCGGCCGACC
1501	GGGAGCGGCA GGAGGAGCTG CAGCGCCAGC TGGAGGATGC CAACCGCGCG CGCCACGGGT
1561	TGGAGACGCA GCACCGGCTG AACCAGCAGC AGCTATCCGA GCTGCGGGCC CAGGTGGAGG
1621	ACCTGCAGAA AGCCCTGCAG GAGCAGGGGG GCAAGACTGA AGATTCCATT TTGCTGAAAA
1681	GGAAGCTGGA GGAACATTTG CAGAAGCTTC ATGAGGCAGA TCTGGAGTTG CAGAGGAAGC
1741	GGGAGTACAT TGAGGAGCTG GAGCCACCCA CTGACAGCAG CACAGCCCGG CGGATCGAGG
1801	AGCTGCAGCA TAACTTGCAG AAGAAGGACG CGGACTTGCG GGCCATGGAG GAGCGATACC
1861	GCCGCTACGT GGACAAGGCC CGCATGGTCA TGCAGACCAT GGAACCCAAG CAGCGGCCAG
1921	CTGCGGGGGC ACCTCCAGAA CTCCATTCCC TGAGGACACA GCTCCGAGAA CGGGATGTCC
1981	GCATCCGACA CCTGGAGATG GACTTTGAGA AAAGCCGAAG TCAGCGGGAG CAGGAAGAAA
2041	AGCTGCTCAT CAGTGCCTGG TATAATATGG GCATGGCCTT GCAGCAGCGA GCTGGGGAGG
2101	AGCGGGCGCC TGCCCATGCC CAGTCATTCC TGGCACAGCA GCGGCTGGCA ACCAATTCTC
2161	GCCGTGGACC CTTGGGACGC CTGGCATCTC TGAACCTTCG CCCCACTGAC AAGCACTGCC
2221	CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
2281	aac

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