Construct: ORF ccsbBroadEn_08500

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017337.1_s300c1, BRDN0000386898
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ECHDC2 (55268)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55268 ECHDC2 enoyl-CoA hydratase domain ... NM_001198961.2 99.7% 99.6% 355A>G;714C>G
2 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_024448153.1 94.2% 94.1% 355A>G;714C>G;751_752ins48
3 human 55268 ECHDC2 enoyl-CoA hydratase domain ... NM_018281.4 89.1% 89% 355A>G;363_364ins93;621C>G
4 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_011541709.2 83.8% 77.1% (many diffs)
5 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_024448160.1 83.6% 83.5% (many diffs)
6 human 55268 ECHDC2 enoyl-CoA hydratase domain ... NM_001198962.1 83.4% 83.2% 355A>G;363_364ins93;607_608ins51
7 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_024448161.1 82.6% 82.5% 355A>G;363_364ins150;564C>G
8 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_024448152.1 74.4% 67.7% (many diffs)
9 human 55268 ECHDC2 enoyl-CoA hydratase domain ... NM_001319958.2 73.4% 73.2% (many diffs)
10 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_024448163.1 73.4% 73.2% (many diffs)
11 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_024448164.1 73.4% 73.2% (many diffs)
12 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_011541713.3 69.8% 63.2% (many diffs)
13 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_011541715.3 69.8% 63.2% (many diffs)
14 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_011541719.2 69.8% 63.2% (many diffs)
15 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_011541720.3 69.8% 63.2% (many diffs)
16 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_011541722.2 69.8% 63.2% (many diffs)
17 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_011541723.2 69.8% 63.2% (many diffs)
18 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_017001638.1 69.8% 63.2% (many diffs)
19 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_017001639.1 69.8% 63.2% (many diffs)
20 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_017001640.1 69.8% 63.2% (many diffs)
21 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_024448157.1 69.8% 63.2% (many diffs)
22 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_024448158.1 69.8% 63.2% (many diffs)
23 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_024448159.1 68.6% 62% (many diffs)
24 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_011541726.3 60.4% 53.9% (many diffs)
25 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XR_002957011.1 59.7% (many diffs)
26 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XM_011541727.3 54.6% 48.1% (many diffs)
27 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XR_002957012.1 49.3% (many diffs)
28 human 55268 ECHDC2 enoyl-CoA hydratase domain ... XR_002957013.1 46.4% (many diffs)
29 mouse 52430 Echdc2 enoyl Coenzyme A hydratase ... NM_026728.4 83.2% 85.1% (many diffs)
30 mouse 52430 Echdc2 enoyl Coenzyme A hydratase ... NM_001254754.1 55.6% 58.9% (many diffs)
31 mouse 52430 Echdc2 enoyl Coenzyme A hydratase ... XM_011240573.1 55.6% 58.9% (many diffs)
32 mouse 52430 Echdc2 enoyl Coenzyme A hydratase ... XM_017320314.1 55.6% 58.9% (many diffs)
33 mouse 52430 Echdc2 enoyl Coenzyme A hydratase ... XM_017320315.1 47.1% 50.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
942
ORF length:
876
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCT GCGCGTTCTG TGCCTCCTGC GCCCCTGGAG GCCCCTTCGG GCCCGCGGCT
121GCGCTTCCGA CGGGGCGGCC GGGGGCTCAG AGATCCAAGT GCGCGCCCTG GCGGGTCCGG
181ACCAAGGGAT CACTGAGATT CTGATGAACA GACCTTCTGC CCGCAATGCC TTGGGGAATG
241TCTTCGTCAG TGAGCTGCTG GAAACTCTGG CCCAGCTGCG GGAGGACCGG CAAGTGCGTG
301TCCTGCTCTT CAGAAGTGGA GTGAAGGGCG TGTTCTGTGC AGGTGCAGAC CTGAAGGAGC
361GGGAACAGAT GAGTGAAGCA GAGGTGGGGG TGTTTGTCCA GCGACTCCGG GGCCTGATGG
421ATGACATCGC AGCCTTCCCT GCACCCACCA TTGCGGCTAT GGATGGGTTT GCCTTGGGCG
481GAGGCCTAGA GCTTGCCCTG GCCTGTGACC TCCGAGTGGC AGCTTCCTCG GCAGTCATGG
541GACTGATTGA GACCACGCGA GGGCTCCTCC CGGGGGCAGG AGGGACTCAG AGGCTGCCCC
601GTTGTCTGGG GGTGGCCCTG GCGAAGGAGC TCATCTTCAC GGGCCGACGA CTGAGTGGAA
661CTGAGGCCCA CGTACTGGGG CTGGTGAATC ACGCTGTGGC CCAGAACGAG GAGGGGGACG
721CCGCCTACCA GCGGGCACGA GCACTGGCCC AGGAGATCCT GCCCCAGGCC CCCATTGCGG
781TGCGGCTGGG CAAAGTAGCC ATTGACCGAG GAACGGAGGT GGACATTGCA TCTGGGATGG
841CCATTGAAGG GATGTGCTAT GCCCAGAATA TTCCAACCCG GGACCGGCTA GAGGGCATGG
901CAGCCTTCAG GGAGAAGCGG ACTCCCAAAT TTGTTGGCAA ATGCCCAACT TTCTTGTACA
961AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac

Download FASTA (ORF) (Full)