Construct: ORF ccsbBroadEn_08503

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010564.1_s300c1, BRDN0000395674
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VPS53 (55275)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55275 VPS53 VPS53 subunit of GARP complex NM_018289.4 99.9% 100% 1437C>T
2 human 55275 VPS53 VPS53 subunit of GARP complex NM_001366253.2 95.8% 95.8% 286_372del;1524C>T
3 human 55275 VPS53 VPS53 subunit of GARP complex NM_001128159.3 80.3% 80% (many diffs)
4 human 55275 VPS53 VPS53 subunit of GARP complex XR_934062.2 77.6% (many diffs)
5 human 55275 VPS53 VPS53 subunit of GARP complex XM_017024817.2 75% 70.3% (many diffs)
6 human 55275 VPS53 VPS53 subunit of GARP complex NM_001366254.2 74.5% 74.1% (many diffs)
7 human 55275 VPS53 VPS53 subunit of GARP complex XM_017024818.1 71.5% 71.1% (many diffs)
8 human 55275 VPS53 VPS53 subunit of GARP complex XR_001752553.2 40.4% (many diffs)
9 human 55275 VPS53 VPS53 subunit of GARP complex XR_934061.3 19.2% (many diffs)
10 mouse 68299 Vps53 VPS53 GARP complex subunit NM_026664.3 72.1% 77.6% (many diffs)
11 mouse 68299 Vps53 VPS53 GARP complex subunit XM_006534052.3 69.1% 74.1% (many diffs)
12 mouse 68299 Vps53 VPS53 GARP complex subunit XM_006534053.3 64.2% 68.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2076
ORF length:
2010
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAT GGAGGAGGAG GAACTGGAGT TCGTGGAGGA GCTGGAAGCC GTGCTGCAGC
121TCACGCCCGA GGTGCAGCTG GCCATCGAGC AGGTGTTTCC AAGCCAGGAC CCTCTAGATC
181GAGCAGATTT CAATGCTGTT GAGTATATCA ATACCCTGTT CCCAACCGAG CAATCTCTGG
241CGAACATAGA CGAAGTCGTG AACAAAATTA GGCTGAAAAT AAGGAGACTG GATGACAATA
301TTCGAACTGT TGTAAGAGGT CAGACGAACG TGGGGCAGGA TGGACGGCAA GTGAAAGAAA
361TCACCCGTGA TATTAAGCAA TTAGATCACG CCAAACGCCA CCTGACCACC TCAATCACCA
421CACTGAACCA CCTGCACATG CTGGCAGGAG GTGTCGACTC CCTCGAAGCC ATGACCAGGC
481GAAGACAATA CGGAGAAGTT GCTAATCTCC TTCAGGGTGT GATGAATGTC CTGGAGCACT
541TCCACAAGTA TATGGGGATT CCGCAGATCC GGCAGCTTTC CGAAAGAGTG AAGGCTGCAC
601AGACTGAGTT AGGACAGCAA ATCCTGGCAG ATTTTGAAGA AGCGTTTCCT TCCCAGGGCA
661CCAAGAGACC AGGAGGACCC AGCAATGTTC TACGAGATGC ATGTCTGGTT GCTAATATTC
721TAGATCCCAG GATCAAACAG GAAATCATCA AAAAGTTTAT TAAACAGCAT CTGTCAGAGT
781ATCTGGTACT TTTTCAAGAA AACCAAGATG TTGCCTGGCT GGACAAAATC GACAGACGCT
841ATGCCTGGAT AAAACGCCAG CTTGTGGACT ATGAGGAGAA ATACGGCCGC ATGTTTCCAC
901GTGAGTGGTG CATGGCTGAG AGGATTGCGG TGGAATTTTG CCATGTGACA AGGGCAGAAC
961TTGCCAAGAT TATGCGTACC AGAGCGAAGG AAATTGAAGT GAAATTGCTT CTTTTTGCTA
1021TTCAAAGAAC AACTAACTTT GAGGGGTTTC TTGCAAAACG CTTCTCCGGC TGCACCCTGA
1081CCGATGGGAC CCTGAAAAAG CTTGAGTCTC CACCCCCATC TACCAATCCC TTCCTGGAAG
1141ATGAGCCAAC ACCAGAGATG GAGGAACTGG CAACGGAGAA AGGAGATTTA GATCAACCAA
1201AGAAGCCTAA AGCCCCAGAC AATCCATTTC ATGGCATTGT TTCCAAGTGT TTTGAGCCTC
1261ATCTCTACGT GTATATCGAA TCCCAAGACA AGAACCTCGG AGAGCTGATA GATCGGTTTG
1321TGGCTGATTT CAAAGCCCAG GGGCCACCTA AGCCCAACAC TGATGAAGGG GGTGCCGTGC
1381TCCCCAGCTG CGCCGACCTC TTTGTCTACT ACAAGAAGTG CATGGTGCAA TGCTCTCAGC
1441TCAGTACTGG GGAGCCCATG ATCGCCCTGA CCACCATTTT CCAGAAGTAC CTCCGAGAAT
1501ATGCCTGGAA AATCCTCTCT GGCAACCTGC CCAAAACCAC AACCAGCAGT GGAGGACTGA
1561CTATCAGCAG CCTCCTCAAG GAAAAGGAGG GCTCAGAAGT AGCCAAGTTC ACTCTGGAGG
1621AGCTCTGCCT CATCTGTAAC ATCCTGAGCA CGGCAGAGTA CTGTCTGGCC ACCACCCAGC
1681AGCTAGAAGA AAAACTCAAA GAAAAAGTGG ATGTAAGTCT GATTGAACGA ATCAATCTGA
1741CTGGAGAGAT GGACACGTTC AGCACCGTCA TCTCCAGCAG TATTCAGCTG CTGGTTCAGG
1801ATCTGGATGC TGCCTGTGAT CCTGCCCTGA CTGCCATGAG CAAGATGCAG TGGCAGAACG
1861TGGAGCACGT TGGTGACCAG AGCCCCTACG TCACCTCTGT CATTCTGCAC ATCAAGCAGA
1921ACGTCCCCAT CATCCGTGAC AACCTGGCTT CCACACGCAA GTACTTCACT CAGTTCTGCG
1981TTAAATTTGC AAACTCCTTC ATTCCCAAAT TCATCACCCA CCTCTTCAAG TGCAAGCCAA
2041TTAGCATGGT GGGAGCAGAA CAGGTGAGAT GGACGTACCC AACTTTCTTG TACAAAGTtg
2101gcattataag aaagcattgc ttatcaattt gttgcaacga ac

Download FASTA (ORF) (Full)