Construct: ORF ccsbBroadEn_08562

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007995.1_s300c1, BRDN0000395185
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDX27 (55661)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55661 DDX27 DEAD-box helicase 27 NM_017895.7 99.9% 99.8% 2111T>C
2 human 55661 DDX27 DEAD-box helicase 27 NM_001348187.1 96.2% 96.1% 1122_1214del;2204T>C
3 mouse 228889 Ddx27 DEAD (Asp-Glu-Ala-Asp) box ... NM_153065.3 84.2% 88.8% (many diffs)
4 mouse 228889 Ddx27 DEAD (Asp-Glu-Ala-Asp) box ... XM_006499422.1 81.5% 85.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2457
ORF length:
2388
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGTACTTGCG CAAAGACGAC GAGGAGGCTG CGAAAAGTTA AGGGCCGGAC
121CGCAGGCTGT GCTCGCTTCC GGAAGTGGCT TCTGCGACAA CATGCTTGCG GACCTCGGCT
181TAATCGGAAC CATAGGCGAG GATGACGAGG TGCCGGTGGA GCCCGAGTCT GACTCCGGGG
241ACGAGGAAGA GGAGGGGCCC ATTGTGCTGG GCAGACGACA AAAAGCTTTG GGGAAGAACC
301GCAGTGCTGA TTTCAACCCT GATTTCGTTT TCACTGAGAA GGAGGGGACG TACGATGGCA
361GCTGGGCCCT GGCTGATGTC ATGAGCCAAC TCAAGAAGAA GAGGGCAGCC ACTACATTAG
421ATGAGAAGAT TGAGAAAGTT CGAAAGAAAA GGAAAACAGA GGATAAAGAA GCCAAGTCTG
481GGAAGTTGGA AAAGGAGAAA GAAGCAAAGG AAGGCTCTGA ACCAAAGGAG CAGGAAGACC
541TTCAAGAGAA TGATGAGGAA GGCTCAGAAG ATGAAGCCTC GGAGACTGAC TACTCATCAG
601CTGATGAGAA CATCCTCACC AAAGCAGATA CACTCAAAGT AAAGGATCGG AAGAAGAAGA
661AGAAGAAAGG ACAGGAAGCA GGAGGATTTT TTGAAGATGC ATCTCAGTAC GATGAAAACC
721TCTCGTTCCA GGACATGAAC CTTTCCCGCC CTCTTCTGAA GGCCATTACA GCCATGGGCT
781TCAAGCAGCC CACCCCGATC CAGAAGGCGT GCATACCTGT GGGTCTATTG GGGAAGGACA
841TCTGTGCCTG TGCAGCCACT GGGACAGGTA AAACTGCCGC CTTTGCCCTG CCTGTTTTGG
901AGCGTCTGAT TTATAAACCC CGCCAGGCTC CAGTCACCCG CGTGCTGGTG CTAGTGCCCA
961CCCGAGAGCT GGGCATCCAG GTGCACTCTG TCACCAGACA GCTGGCCCAG TTCTGCAACA
1021TCACCACCTG CCTGGCTGTG GGCGGCTTGG ATGTGAAGTC TCAGGAAGCA GCTCTTCGGG
1081CAGCGCCTGA CATCCTCATC GCCACCCCAG GCCGGCTCAT CGATCACCTC CACAACTGCC
1141CTTCCTTCCA CCTGAGCAGC ATCGAGGTGC TCATCCTGGA CGAGGCTGAC AGGATGCTGG
1201ATGAGTACTT TGAGGAGCAG ATGAAGGAGA TCATCCGAAT GTGTTCCCAC CACCGCCAGA
1261CCATGCTCTT CTCGGCCACC ATGACAGACG AGGTGAAAGA TCTGGCTTCT GTCTCCTTGA
1321AGAATCCTGT CCGGATATTT GTGAACAGCA ACACAGATGT GGCTCCCTTC CTGCGGCAGG
1381AGTTCATCCG GATCCGGCCT AATCGTGAAG GAGACCGGGA AGCCATCGTG GCAGCTTTGT
1441TGACGAGGAC CTTCACTGAC CATGTGATGC TGTTCACGCA AACCAAGAAG CAGGCCCACC
1501GCATGCACAT CCTCCTGGGG CTCATGGGGC TGCAGGTGGG TGAGCTCCAT GGCAACTTGT
1561CACAGACGCA GCGGCTGGAG GCCCTCCGGC GTTTTAAGGA TGAACAGATT GACATCCTCG
1621TGGCCACTGA TGTGGCAGCC CGTGGACTTG ACATTGAGGG GGTCAAAACG GTAATCAACT
1681TCACAATGCC TAATACCATC AAACATTATG TCCACCGGGT GGGGCGAACA GCACGTGCTG
1741GCAGGGCTGG GCGCTCAGTC TCTCTGGTGG GAGAAGATGA GCGGAAGATG CTGAAGGAGA
1801TTGTAAAAGC TGCCAAGGCC CCTGTGAAGG CCAGGATACT TCCCCAAGAT GTCATCCTCA
1861AATTCCGGGA CAAGATTGAG AAAATGGAGA AAGATGTGTA TGCAGTTCTG CAGCTAGAGG
1921CGGAGGAAAA AGAGATGCAG CAGTCAGAAG CCCAGATCAA TACAGCAAAG CGGCTCCTGG
1981AGAAGGGGAA GGAGGCAGTG GTCCAAGAGC CCGAGAGGAG CTGGTTCCAG ACCAAAGAAG
2041AGAGGAAGAA GGAGAAAATT GCCAAAGCTC TGCAGGAATT TGACTTGGCC TTAAGAGGAA
2101AGAAGAAAAG GAAGAAGTTT ATGAAGGATG CCAAAAAAAA GGGGGAGATG ACAGCAGAGG
2161AAAGGTCTCA GTTTGAAACC CTCAAGGCGC AGATGTTTGC TGAACGGCTA GCGAAGAGGA
2221ATCGCAGAGC CAAGCGGGCC CGAGCAATGC CCGAGGAGGA GCCAGTGAGA GGTCCTGCCA
2281AGAAGCAAAA GCAGGGGAAG AAATCTGTAT TTGATGAAGA ACTCACCAAC ACAAGCAAGA
2341AGGCCCTGAA ACAGTATCGA GCTGGCCCTT CCTTTGAAGA AAGGAAACAG TTGGGCTTGC
2401CCCACCAGAG ACGAGGAGGA AACTTTAAAT CTAAATCCAG ATACAAGAGG AGGAAGTTGC
2461CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
2521aac

Download FASTA (ORF) (Full)