Transcript: Mouse NM_153065.3

Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Ddx27 (228889)
Length:
2592
CDS:
22..2304

Additional Resources:

NCBI RefSeq record:
NM_153065.3
NBCI Gene record:
Ddx27 (228889)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_153065.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000071091 GCCAATCCAGAAGGCGTGTAT pLKO.1 645 CDS 100% 4.950 3.960 N Ddx27 n/a
2 TRCN0000349187 GCCAATCCAGAAGGCGTGTAT pLKO_005 645 CDS 100% 4.950 3.960 N Ddx27 n/a
3 TRCN0000071090 ACTACACTAGATGAGAAGATT pLKO.1 271 CDS 100% 5.625 3.938 N Ddx27 n/a
4 TRCN0000316450 ACTACACTAGATGAGAAGATT pLKO_005 271 CDS 100% 5.625 3.938 N Ddx27 n/a
5 TRCN0000071092 ACTGAGAAGGAGGGCATGTAT pLKO.1 193 CDS 100% 5.625 3.938 N Ddx27 n/a
6 TRCN0000316451 ACTGAGAAGGAGGGCATGTAT pLKO_005 193 CDS 100% 5.625 3.938 N Ddx27 n/a
7 TRCN0000071088 GCTGACTTCAACCCTGACTTT pLKO.1 166 CDS 100% 4.950 3.465 N Ddx27 n/a
8 TRCN0000316449 GCTGACTTCAACCCTGACTTT pLKO_005 166 CDS 100% 4.950 3.465 N Ddx27 n/a
9 TRCN0000071089 GATGAGGAAATCCTCACCAAA pLKO.1 457 CDS 100% 0.495 0.347 N Ddx27 n/a
10 TRCN0000316379 GATGAGGAAATCCTCACCAAA pLKO_005 457 CDS 100% 0.495 0.347 N Ddx27 n/a
11 TRCN0000201113 CCTGAGTTCAATTCTCAGCAA pLKO.1 2447 3UTR 100% 0.264 0.132 Y Ripply3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_153065.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15907 pDONR223 0% 87.5% 92.4% None (many diffs) n/a
2 ccsbBroad304_15907 pLX_304 0% 87.5% 92.4% V5 (many diffs) n/a
3 TRCN0000474851 AGATCTTTTATGCAACTTCCCCCC pLX_317 16.3% 87.5% 92.4% V5 (not translated due to frame shift) (many diffs) n/a
4 ccsbBroadEn_08562 pDONR223 100% 84.2% 88.8% None (many diffs) n/a
5 ccsbBroad304_08562 pLX_304 0% 84.2% 88.8% V5 (many diffs) n/a
6 TRCN0000475485 CTGGCCTTTGCCATGGGTGGATAA pLX_317 15.3% 84.2% 88.8% V5 (many diffs) n/a
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