Construct: ORF ccsbBroadEn_08857

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001212.1_s300c1, BRDN0000396552
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CPEB1 (64506)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001079534.2 99.9% 100% 1290A>G
2 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001079535.2 99.9% 100% 1290A>G
3 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001288819.1 99.9% 100% 1290A>G
4 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001365244.1 99.9% 100% 1290A>G
5 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001365245.1 99.9% 100% 1290A>G
6 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001365246.1 99.9% 100% 1290A>G
7 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001365247.1 99.9% 100% 1290A>G
8 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001365248.1 99.9% 100% 1290A>G
9 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001365249.1 99.9% 100% 1290A>G
10 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001079533.2 98.9% 98.9% 835_849del;1305A>G
11 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001365250.1 98.9% 98.9% 835_849del;1305A>G
12 human 64506 CPEB1 cytoplasmic polyadenylation... NM_030594.5 86.5% 86.6% 1_225del;1515A>G
13 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001365243.1 85.8% 85.8% 1_225del;1060_1074del;1530A>G
14 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001365240.1 82.5% 82.6% 1_306del;1596A>G
15 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001365241.1 82.5% 82.6% 1_306del;1596A>G
16 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001365242.1 82.5% 82.6% 1_306del;1596A>G
17 human 64506 CPEB1 cytoplasmic polyadenylation... NM_001288820.2 68.8% 68.9% 0_1ins453;837A>G
18 mouse 12877 Cpeb1 cytoplasmic polyadenylation... XM_011250790.2 90.4% 94.7% (many diffs)
19 mouse 12877 Cpeb1 cytoplasmic polyadenylation... XM_017321964.1 90.4% 94.7% (many diffs)
20 mouse 12877 Cpeb1 cytoplasmic polyadenylation... XM_017321965.1 87.2% 89.1% (many diffs)
21 mouse 12877 Cpeb1 cytoplasmic polyadenylation... XM_011250792.1 80% 84.3% (many diffs)
22 mouse 12877 Cpeb1 cytoplasmic polyadenylation... NM_001252526.1 79.1% 82.8% (many diffs)
23 mouse 12877 Cpeb1 cytoplasmic polyadenylation... NM_007755.5 78.4% 82.1% (many diffs)
24 mouse 12877 Cpeb1 cytoplasmic polyadenylation... NM_001252525.1 78.3% 82% (many diffs)
25 mouse 12877 Cpeb1 cytoplasmic polyadenylation... XM_011250787.2 74.1% 77.6% (many diffs)
26 mouse 12877 Cpeb1 cytoplasmic polyadenylation... XM_011250786.2 74% 77.5% (many diffs)
27 mouse 12877 Cpeb1 cytoplasmic polyadenylation... XM_011250785.2 73.5% 76.9% (many diffs)
28 mouse 12877 Cpeb1 cytoplasmic polyadenylation... XM_011250784.2 73.3% 76.8% (many diffs)
29 mouse 12877 Cpeb1 cytoplasmic polyadenylation... XM_011250788.2 72% 75.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1524
ORF length:
1458
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCT TTTCCCAACC TCTGCGCAAG AATCTTCCCG TGGCCTCCCA GATGCAAATG
121ACTTGTGCCT TGGCCTGCAG TCCCTCAGTC TGACAGGCTG GGACCGACCC TGGAGCACCC
181AGGACTCAGA TTCCTCAGCC CAGAGCAGCA CACACTCGGT ACTGAGCATG CTCCATAACC
241CACTGGGAAA TGTCCTAGGA AAACCCCCCT TGAGCTTCCT GCCTCTGGAT CCCCTTGGGT
301CTGACTTGGT GGACAAGTTT CCAGCACCCT CAGTTAGAGG ATCACGCCTG GACACCCGGC
361CCATCCTGGA CTCTCGATCT AGCAGCCCCT CTGACTCAGA CACCAGTGGC TTCAGCTCTG
421GATCAGATCA TCTCTCAGAT TTGATTTCAA GCCTTCGCAT TTCTCCACCT CTGCCCTTCC
481TGTCTCTGTC AGGGGGTGGT CCCAGAGACC CTTTAAAGAT GGGGGTAGGG TCTCGGATGG
541ACCAAGAGCA AGCTGCTCTT GCTGCAGTCA CTCCCTCCCC AACCAGTGCT TCAAAGAGAT
601GGCCAGGAGC TTCTGTGTGG CCATCCTGGG ACCTCCTCGA AGCTCCCAAA GACCCCTTCA
661GCATAGAGAG AGAGGCCAGG CTGCACCGAC AAGCTGCAGC TGTGAATGAA GCCACCTGTA
721CCTGGAGTGG CCAGCTTCCT CCCCGGAACT ATAAGAACCC CATCTACTCT TGCAAGGTGT
781TTCTAGGAGG TGTTCCTTGG GATATTACAG AAGCTGGATT AGTTAACACC TTCCGTGTTT
841TTGGCTCTTT GAGTGTGGAG TGGCCTGGTA AGGATGGCAA GCATCCCCGG TGTCCTCCCA
901AAGGGTATGT GTATCTGGTC TTCGAACTAG AGAAGTCTGT CCGATCCTTG CTTCAGGCTT
961GCTCTCATGA CCCGCTGAGC CCAGATGGCC TGAGTGAATA TTATTTCAAG ATGTCCAGCC
1021GAAGGATGCG CTGCAAGGAG GTGCAGGTGA TCCCCTGGGT ATTAGCCGAC AGTAACTTTG
1081TCCGGAGCCC ATCTCAGAGG CTTGACCCCA GCAGGACGGT GTTTGTCGGT GCTCTGCATG
1141GAATGCTAAA TGCTGAGGCC CTGGCAGCCA TCTTGAACGA CCTATTTGGT GGAGTGGTGT
1201ATGCCGGGAT TGACACAGAT AAGCACAAGT ATCCCATTGG TTCTGGTCGT GTGACTTTCA
1261ATAACCAACG GAGTTACCTG AAAGCAGTCA GCGCTGCTTT TGTGGAGATC AAAACCACCA
1321AGTTCACAAA GAAGGTTCAG ATTGACCCCT ACCTGGAAGA TTCTCTGTGT CATATCTGCA
1381GTTCTCAGCC TGGTCCTTTC TTCTGTCGAG ATCAGGTCTG CTTCAAATAC TTCTGCCGGA
1441GCTGCTGGCA CTGGCGGCAC AGCATGGAGG GCCTGCGCCA CCACAGCCCC CTGATGCGGA
1501ACCAGAAGAA CCGAGATTCC AGCTACCCAA CTTTCTTGTA CAAAGTtggc attataagaa
1561agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)