Construct: ORF ccsbBroadEn_09052

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011373.1_s300c1, BRDN0000457234
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDHD1 (80821)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80821 DDHD1 DDHD domain containing 1 NM_030637.3 99.9% 99.8% 2024A>G
2 human 80821 DDHD1 DDHD domain containing 1 NM_001160147.2 99.1% 99% 1011_1031del;2045A>G
3 human 80821 DDHD1 DDHD domain containing 1 NM_001160148.2 96.8% 96.6% 2024A>G;2438_2521del
4 human 80821 DDHD1 DDHD domain containing 1 XM_017021668.2 96.2% 96.1% 1012_1113del;2126A>G
5 human 80821 DDHD1 DDHD domain containing 1 XM_005268103.3 96.1% 95.9% 1011_1031del;2045A>G;2459_2542del
6 human 80821 DDHD1 DDHD domain containing 1 XM_011537189.3 95.4% 95.3% 1012_1134del;2147A>G
7 human 80821 DDHD1 DDHD domain containing 1 XM_017021669.2 95% 94.9% 1011_1012ins129;1895A>G
8 human 80821 DDHD1 DDHD domain containing 1 XM_005268102.3 93.3% 93.1% 1012_1113del;2126A>G;2540_2623del
9 human 80821 DDHD1 DDHD domain containing 1 XM_011537188.3 92.6% 92.4% 1012_1134del;2147A>G;2561_2644del
10 human 80821 DDHD1 DDHD domain containing 1 XM_005268105.3 92% 91.8% 1011_1012ins129;1895A>G;2309_2392del
11 mouse 114874 Ddhd1 DDHD domain containing 1 NM_001039106.3 85.6% 88.6% (many diffs)
12 mouse 114874 Ddhd1 DDHD domain containing 1 NM_176845.5 83% 85.7% (many diffs)
13 mouse 114874 Ddhd1 DDHD domain containing 1 NM_001042719.2 80% 82.6% (many diffs)
14 mouse 114874 Ddhd1 DDHD domain containing 1 NM_001284399.1 50.1% 53.4% (many diffs)
15 mouse 100628600 Mir5131 microRNA 5131 NR_039593.1 1.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2682
ORF length:
2616
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA TTACCCGGGC CGCGGGTCCC CACGGAGCCC CGAGCATAAC GGCCGAGGCG
121GCGGCGGCGG CGCCTGGGAG CTGGGCTCAG ACGCGAGGCC AGCGTTCGGC GGCGGCGTCT
181GCTGCTTCGA GCACCTGCCC GGCGGGGACC CGGACGACGG CGACGTGCCC CTGGCCCTGC
241TGCGCGGGGA ACCCGGGCTG CATTTGGCGC CGGGCACCGA CGACCACAAC CACCACCTCG
301CGCTGGACCC CTGCCTCAGT GACGAGAACT ATGACTTCAG CTCCGCCGAG TCGGGCTCCT
361CGCTGCGCTA CTACAGCGAG GGTGAGAGCG GCGGCGGCGG CAGCTCCTTG TCGCTGCACC
421CGCCGCAGCA GCCTCCGCTG GTCCCGACGA ACTCGGGGGG CGGCGGCGCG ACAGGAGGGT
481CCCCCGGGGA AAGGAAACGT ACCCGGCTTG GCGGCCCGGC GGCCCGGCAC CGCTATGAGG
541TAGTGACGGA GCTGGGCCCG GAGGAGGTAC GCTGGTTCTA CAAGGAGGAC AAGAAGACCT
601GGAAGCCCTT CATCGGCTAC GACTCGCTCC GCATCGAGCT CGCCTTCCGG ACCCTGCTGC
661AGACCACGGG TGCCCGGCCC CAGGGCGGGG ACCGGGACGG CGACCATGTG TGCTCCCCCA
721CGGGCCCAGC CTCCAGTTCC GGAGAAGATG ACGATGAGGA CCGCGCCTGC GGCTTCTGCC
781AGAGTACGAC GGGGCACGAG CCGGAGATGG TGGAGCTTGT GAACATCGAG CCTGTGTGCG
841TGCGGGGCGG CCTCTACGAG GTGGATGTGA CCCAAGGAGA GTGCTACCCG GTGTACTGGA
901ACCAGGCTGA TAAAATACCA GTAATGCGTG GACAGTGGTT TATTGACGGC ACTTGGCAGC
961CTCTAGAAGA GGAAGAAAGT AATTTAATTG AGCAAGAACA TCTCAATTGT TTTAGGGGCC
1021AGCAGATGCA GGAAAATTTC GATATTGAAG TGTCAAAATC CATAGATGGA AAAGATGCTG
1081TTCATAGTTT CAAGTTGAGT CGAAACCATG TGGACTGGCA CAGTGTGGAT GAAGTATATC
1141TTTATAGTGA TGCAACAACA TCTAAAATTG CAAGAACAGT TACCCAAAAA CTGGGATTTT
1201CTAAAGCATC AAGTAGTGGT ACCAGACTTC ATAGAGGTTA TGTAGAAGAA GCCACATTAG
1261AAGACAAGCC ATCACAGACT ACCCATATTG TATTTGTTGT GCATGGCATT GGGCAGAAAA
1321TGGACCAAGG AAGAATTATC AAAAATACAG CTATGATGAG AGAAGCTGCA AGAAAAATAG
1381AAGAAAGGCA TTTTTCCAAC CATGCAACAC ATGTTGAATT TCTGCCTGTT GAGTGGCGGT
1441CAAAACTTAC TCTTGATGGA GACACTGTTG ATTCCATTAC TCCTGACAAA GTACGAGGTT
1501TAAGGGATAT GCTGAACAGC AGTGCAATGG ACATAATGTA TTATACTAGT CCACTTTATA
1561GAGATGAACT AGTTAAAGGC CTTCAGCAAG AGCTGAATCG ATTGTATTCC CTTTTCTGTT
1621CTCGGAATCC AGACTTTGAA GAAAAAGGGG GTAAAGTCTC AATAGTATCA CATTCCTTGG
1681GATGTGTAAT TACTTATGAC ATAATGACTG GCTGGAATCC AGTTCGGCTG TATGAACAGT
1741TGCTGCAAAA GGAAGAAGAG TTGCCTGATG AACGATGGAT GAGCTATGAA GAACGACATC
1801TTCTTGATGA ACTCTATATA ACTAAACGAC GGCTGAAGGA AATAGAAGAA CGGCTTCACG
1861GATTGAAAGC ATCATCTATG ACACAAACAC CTGCCTTAAA ATTTAAGGTT GAGAATTTCT
1921TCTGTATGGG ATCCCCATTA GCAGTTTTCT TGGCGTTGCG TGGCATCCGC CCAGGAAATA
1981CTGGAAGTCA AGACCATATT TTGCCTAGAG AGATTTGTAA CCGGTTACTA AATATTTTTC
2041ATCCTACAGA TCCAGTGGCT TATAGATTAG AACCATTAAT ACTGAAACGC TACAGCAACA
2101TTTCACCTGT CCAGATCCAC TGGTACAATA CTTCAAATCC TTTACCTTAT GAACATATGA
2161AGCCAAGCTT TCTCAACCCA GCTAAAGAAC CTACCTCAGT TTCAGAGAAT GAAGGCATTT
2221CAACCATACC AAGCCCTGTG ACCTCACCAG TTTTGTCCCG CCGACACTAT GGAGAATCTA
2281TAACAAATAT AGGCAAAGCA AGCATATTAG GGGCTGCTAG CATTGGAAAG GGACTTGGAG
2341GAATGTTGTT CTCAAGATTT GGACGTTCAT CTACAACACA GTCATCTGAA ACATCAAAAG
2401ACTCAATGGA AGATGAGAAG AAGCCAGTTG CCTCACCTTC TGCTACCACC GTAGGGACAC
2461AGACCCTTCC ACATAGCAGT TCTGGCTTCC TCGATTCTGC ATTGGAGTTG GATCACAGGA
2521TTGATTTTGA ACTCAGAGAA GGCCTTGTGG AGAGCCGCTA TTGGTCAGCT GTCACGTCGC
2581ATACTGCCTA TTGGTCATCC TTGGATGTTG CCCTTTTTCT TTTAACCTTC ATGTATAAAC
2641ATGAGCACGA TGATGATGCA AAACCCAATT TAGATCCAAT CTGCCCAACT TTCTTGTACA
2701AAGTTGGcat tataagaaag cattgcttat caatttgttg caacgaac

Download FASTA (ORF) (Full)

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