Transcript: Human XM_005268105.3

PREDICTED: Homo sapiens DDHD domain containing 1 (DDHD1), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DDHD1 (80821)
Length:
12812
CDS:
226..2799

Additional Resources:

NCBI RefSeq record:
XM_005268105.3
NBCI Gene record:
DDHD1 (80821)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005268105.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000431564 TTGTAACCGGTTACTAAATAT pLKO_005 2046 CDS 100% 15.000 21.000 N DDHD1 n/a
2 TRCN0000427267 AGTCTCAATAGTATCACATTC pLKO_005 1686 CDS 100% 10.800 15.120 N DDHD1 n/a
3 TRCN0000051023 GCCGACACTATGGAGAATCTA pLKO.1 2291 CDS 100% 5.625 7.875 N DDHD1 n/a
4 TRCN0000414560 CACGTCGCATACTGCCTATTG pLKO_005 2688 CDS 100% 10.800 8.640 N DDHD1 n/a
5 TRCN0000051025 GCCATCACAGACTACCCATAT pLKO.1 1299 CDS 100% 10.800 8.640 N DDHD1 n/a
6 TRCN0000434626 AGCAGTGCAATGGACATAATG pLKO_005 1549 CDS 100% 13.200 9.240 N DDHD1 n/a
7 TRCN0000418646 AGTTCTGAAGAAGTCATTTAC pLKO_005 2965 3UTR 100% 13.200 9.240 N DDHD1 n/a
8 TRCN0000421575 TTGGGATGTGTAATTACTTAT pLKO_005 1708 CDS 100% 13.200 9.240 N DDHD1 n/a
9 TRCN0000422466 CCAGATCCACTGGTACAATAC pLKO_005 2142 CDS 100% 10.800 7.560 N DDHD1 n/a
10 TRCN0000051024 CCTGACAAAGTACGAGGTTTA pLKO.1 1513 CDS 100% 10.800 7.560 N DDHD1 n/a
11 TRCN0000051027 CCAGGAAATACTGGAAGTCAA pLKO.1 2002 CDS 100% 4.950 3.465 N DDHD1 n/a
12 TRCN0000051026 CGGCTGAAGGAAATAGAAGAA pLKO.1 1861 CDS 100% 4.950 3.465 N DDHD1 n/a
13 TRCN0000313463 AGCAAGAGCTGAATCGATTAT pLKO_005 1616 CDS 100% 13.200 9.240 N Ddhd1 n/a
14 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 5860 3UTR 100% 13.200 6.600 Y LIAS n/a
15 TRCN0000166364 CACACACACACACACACACAA pLKO.1 6146 3UTR 100% 4.950 2.475 Y KAAG1 n/a
16 TRCN0000074123 CGCCTGTAATCCCAACACTTT pLKO.1 4063 3UTR 100% 4.950 2.475 Y GJD4 n/a
17 TRCN0000166650 CGCCTGTAATCCCAACACTTT pLKO.1 4063 3UTR 100% 4.950 2.475 Y C9orf85 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005268105.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09052 pDONR223 100% 92% 91.8% None 1011_1012ins129;1895A>G;2309_2392del n/a
2 ccsbBroad304_09052 pLX_304 0% 92% 91.8% V5 1011_1012ins129;1895A>G;2309_2392del n/a
3 TRCN0000477591 ATTCCCAATCATCACCCGATGCTC pLX_317 18.8% 92% 91.8% V5 1011_1012ins129;1895A>G;2309_2392del n/a
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