Construct: ORF ccsbBroadEn_09564

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015582.1_s300c1, BRDN0000456532
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRIM50 (135892)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 135892 TRIM50 tripartite motif containing 50 NM_001281451.1 100% 100%
2 human 135892 TRIM50 tripartite motif containing 50 NM_178125.3 100% 100%
3 human 135892 TRIM50 tripartite motif containing 50 NM_001281450.1 99.7% 99.7% 725_726insGAA
4 human 135892 TRIM50 tripartite motif containing 50 XM_011515789.2 99.7% 99.7% 725_726insGAA
5 human 135892 TRIM50 tripartite motif containing 50 XM_011515787.1 61.6% 60.3% (many diffs)
6 human 135892 TRIM50 tripartite motif containing 50 XM_011515788.2 57% 57% 0_1ins627
7 human 375593 TRIM73 tripartite motif containing 73 NM_198924.3 49.4% 47.8% (many diffs)
8 human 378108 TRIM74 tripartite motif containing 74 NM_001317815.1 49.2% 47.6% (many diffs)
9 human 378108 TRIM74 tripartite motif containing 74 NM_198853.3 49.2% 47.6% (many diffs)
10 human 378108 TRIM74 tripartite motif containing 74 XM_011516187.3 49.2% 47.6% (many diffs)
11 human 378108 TRIM74 tripartite motif containing 74 XM_011516189.2 49% 47.4% (many diffs)
12 human 378108 TRIM74 tripartite motif containing 74 XM_011516190.2 44.2% 39.2% (many diffs)
13 mouse 215061 Trim50 tripartite motif-containing 50 NM_001346745.1 82.8% 87.6% (many diffs)
14 mouse 215061 Trim50 tripartite motif-containing 50 XM_006504411.3 82.8% 87.6% (many diffs)
15 mouse 215061 Trim50 tripartite motif-containing 50 NM_178240.2 82.6% 87.4% (many diffs)
16 mouse 215061 Trim50 tripartite motif-containing 50 XM_006504412.3 82.6% 87.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1530
ORF length:
1461
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCTTGGCAG GTGAGCCTGC CAGAGCTGGA GGACCGGCTT CAGTGTCCCA
121TCTGCCTGGA GGTCTTCAAG GAGCCCCTGA TGCTGCAGTG TGGCCACTCT TACTGCAAGG
181GCTGCCTGGT TTCCCTGTCC TGCCACCTGG ATGCCGAGCT GCGCTGCCCC GTGTGCCGGC
241AGGCGGTGGA CGGCAGCAGC TCCCTGCCCA ACGTCTCCCT GGCCAGGGTG ATCGAAGCCC
301TGAGGCTCCC TGGGGACCCG GAGCCCAAGG TCTGCGTGCA CCACCGGAAC CCGCTCAGCC
361TTTTCTGCGA GAAGGACCAG GAGCTCATCT GTGGCCTCTG CGGTCTGCTG GGCTCCCACC
421AACACCACCC GGTCACGCCC GTCTCCACCG TCTACAGCCG CATGAAGGAG GAGCTCGCAG
481CCCTCATCTC TGAGCTGAAG CAGGAGCAGA AAAAGGTGGA TGAGCTCATC GCCAAACTGG
541TGAACAACCG GACCCGAATC GTCAATGAGT CGGATGTCTT CAGCTGGGTG ATCCGCCGCG
601AGTTCCAGGA GCTGCACCAC CTGGTGGATG AGGAGAAGGC CCGCTGCCTG GAGGGGATAG
661GGGGTCACAC CCGTGGCCTG GTGGCCTCCC TGGACATGCA GCTGGAGCAG GCCCAGGGAA
721CCCGGGAGCG GCTGGCCCAA GCCGAGTGTG TGCTGGAACA GTTCGGCAAT GAGGACCACC
781ACAAGTTCAT CCGGAAGTTC CACTCCATGG CCTCCAGAGC AGAGATGCCG CAGGCCCGGC
841CCTTAGAAGG CGCATTCAGC CCCATCTCCT TCAAGCCAGG CCTCCACCAG GCTGACATCA
901AGCTGACCGT GTGGAAAAGG CTCTTCCGGA AAGTTTTGCC AGCCCCGGAG CCTCTCAAGT
961TGGACCCTGC CACTGCCCAC CCACTCCTGG AGCTCTCCAA GGGCAACACG GTGGTGCAGT
1021GCGGGCTTCT GGCCCAGCGG CGAGCCAGCC AGCCTGAGCG CTTCGACTAC AGCACCTGCG
1081TCCTGGCCAG CCGCGGCTTC TCCTGCGGCC GCCACTACTG GGAGGTGGTG GTGGGCAGCA
1141AGAGCGACTG GCGCCTGGGG GTCATCAAGG GCACAGCCAG CCGTAAGGGC AAGCTGAACA
1201GGTCCCCCGA GCACGGCGTG TGGCTGATCG GCCTGAAGGA GGGCCGGGTG TACGAAGCCT
1261TTGCCTGCCC CCGGGTACCC CTGCCCGTGG CCGGCCACCC CCACCGCATC GGGCTCTACC
1321TGCACTATGA GCAGGGCGAA CTCACCTTCT TCGATGCCGA CCGCCCCGAT GACCTGCGGC
1381CGCTCTACAC CTTCCAGGCC GACTTCCAGG GCAAGCTCTA CCCCATCCTG GACACCTGCT
1441GGCACGAGAG GGGCAGCAAC TCGCTGCCCA TGGTGCTGCC CCCGCCCAGC GGGCCTGGCC
1501CCCTCAGCCC CGAGCAGCCC ACCAAGCTGT TGCCAACTTT CTTGTACAAA GTTGGcatta
1561taagaaagca ttgcttatca atttgttgca acgaac

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.