Construct: ORF ccsbBroadEn_09808

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002080.1_s300c1, BRDN0000397859
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALG1L (200810)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 200810 ALG1L ALG1 chitobiosyldiphosphodo... NM_001015050.2 99.8% 99.4% 403A>G
2 human 200810 ALG1L ALG1 chitobiosyldiphosphodo... XM_017005846.2 99.2% 98.9% 158_160delCAG;406A>G
3 human 200810 ALG1L ALG1 chitobiosyldiphosphodo... XM_017005847.1 99.2% 98.9% 158_160delCAG;406A>G
4 human 200810 ALG1L ALG1 chitobiosyldiphosphodo... NM_001195223.1 90.1% 89.8% 1_60del;463A>G
5 human 200810 ALG1L ALG1 chitobiosyldiphosphodo... XM_017005844.2 89.7% 89.4% 1_60del;218_220delCAG;466A>G
6 human 200810 ALG1L ALG1 chitobiosyldiphosphodo... XM_017005845.1 89.7% 89.4% 1_60del;218_220delCAG;466A>G
7 human 200810 ALG1L ALG1 chitobiosyldiphosphodo... XM_024453384.1 83.6% 82.3% (many diffs)
8 human 200810 ALG1L ALG1 chitobiosyldiphosphodo... XM_024453383.1 83.1% 81.9% (many diffs)
9 human 644974 ALG1L2 ALG1 chitobiosyldiphosphodo... NM_001136152.1 41.1% 37% (many diffs)
10 human 56052 ALG1 ALG1 chitobiosyldiphosphodo... NM_001330504.1 40% 38.3% (many diffs)
11 human 56052 ALG1 ALG1 chitobiosyldiphosphodo... XM_017023458.1 40% 38.3% (many diffs)
12 human 650368 TSSC2 tumor suppressing subtransf... NR_024248.1 36.4% (many diffs)
13 human 56052 ALG1 ALG1 chitobiosyldiphosphodo... XM_017023457.2 31.8% 30.4% (many diffs)
14 human 56052 ALG1 ALG1 chitobiosyldiphosphodo... NM_019109.5 31% 29.6% (many diffs)
15 human 56052 ALG1 ALG1 chitobiosyldiphosphodo... XR_932882.3 27.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
627
ORF length:
561
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA GCGGTCGGCC TTCATGGAGC TGGATGCTGG GAGCAGGCTG GTGATGCATC
121TCCGCGAGTG GCCAGCCCTG CTGGTCAGCA GCACGGGCTG GACAGAGTTT GAACAACTTA
181CTCTTGATGG ACACAACCTT CCTTCTCTTG TCTGTGTGAT AACAGGGTCG GTGGACCTGG
241GTGTCTGTCT GCACATGTCC TCCAGTGGCC TGGACCTGCC CATGAAGGTG GTGGACATGT
301TCGGGTGCTG TTTGCCTGTG TGTGCCGTGA ACTTCAAGTG TTTACATGAG CTGGTGAAAC
361ATGAAGAAAA TGGCCTGGTC TTTGAGGACT CAGAGGAACT GGCAGCTCTG CAGATGCTTT
421TCTCAAACTT TCCTGATCCT GCAGGCAAGC TAAACCAGTT TTGGAAGGAC CTGCGGGAGT
481CGCAGCAGCT CCGATGGGAT GAGAGCTGGG TGCAGACTGT GCTCCCTTTG GTTATGGACA
541TACAACTCCT GGGCCAGAGG CTAAAACCCC GGGACCCCTG CTGTCCTTCC CGCAGCTTCT
601TCTCGGAGTC TCAGGGCAAA CCCTTTTGCC CAACTTTCTT GTACAAAGTt ggcattataa
661gaaagcattg cttatcaatt tgttgcaacg aac

Download FASTA (ORF) (Full)