Construct: ORF ccsbBroadEn_10432

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003771.1_s300c1, BRDN0000398000
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HADHB (3032)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3032 HADHB hydroxyacyl-CoA dehydrogena... NM_000183.3 99.7% 99.5% 3_4insACT
2 human 3032 HADHB hydroxyacyl-CoA dehydrogena... XM_011532803.1 99.7% 99.5% 3_4insACT
3 human 3032 HADHB hydroxyacyl-CoA dehydrogena... NM_001281512.1 96.6% 96.4% 3_4insACT;208_209ins45
4 human 3032 HADHB hydroxyacyl-CoA dehydrogena... XM_024452830.1 95.3% 94.7% 0_1ins50;18_34del
5 human 3032 HADHB hydroxyacyl-CoA dehydrogena... NM_001281513.2 94% 93% (many diffs)
6 human 3032 HADHB hydroxyacyl-CoA dehydrogena... XM_024452831.1 94% 93% (many diffs)
7 mouse 231086 Hadhb hydroxyacyl-Coenzyme A dehy... NM_001289798.1 89% 91.1% (many diffs)
8 mouse 231086 Hadhb hydroxyacyl-Coenzyme A dehy... NM_001289799.1 89% 91.1% (many diffs)
9 mouse 231086 Hadhb hydroxyacyl-Coenzyme A dehy... NM_145558.2 89% 91.1% (many diffs)
10 mouse 231086 Hadhb hydroxyacyl-Coenzyme A dehy... XM_017320828.1 89% 91.1% (many diffs)
11 mouse 231086 Hadhb hydroxyacyl-Coenzyme A dehy... XM_017320829.1 89% 91.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1491
ORF length:
1425
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAC TACTATCTTG ACTTACCCCT TTAAAAATCT TCCCACTGCA TCAAAATGGG
121CCCTCAGATT TTCCATAAGA CCTCTGAGCT GTTCCTCCCA GCTACGAGCT GCCCCAGCTG
181TCCAGACCAA AACGAAGAAG ACGTTAGCCA AACCCAATAT AAGGAATGTT GTGGTGGTGG
241ATGGTGTTCG CACTCCATTT TTGCTGTCTG GCACTTCATA TAAAGACCTG ATGCCACATG
301ATTTGGCTAG AGCAGCGCTT ACGGGTTTGT TGCATCGGAC CAGTGTCCCT AAGGAAGTAG
361TTGATTATAT CATCTTTGGT ACAGTTATTC AGGAAGTGAA AACAAGCAAT GTGGCTAGAG
421AGGCTGCCCT TGGAGCTGGC TTCTCTGACA AGACTCCTGC TCACACTGTC ACCATGGCTT
481GTATCTCTGC CAACCAAGCC ATGACCACAG GTGTTGGCTT GATTGCTTCT GGCCAGTGTG
541ATGTGATCGT GGCAGGTGGT GTTGAGTTGA TGTCCGATGT CCCTATTCGT CACTCAAGGA
601AAATGAGAAA ACTGATGCTT GATCTCAATA AGGCCAAATC TATGGGCCAG CGACTGTCTT
661TAATCTCTAA ATTCCGATTT AATTTCCTAG CACCTGAGCT CCCTGCGGTT TCTGAGTTCT
721CCACCAGTGA GACCATGGGC CACTCTGCAG ACCGACTGGC CGCTGCCTTT GCTGTTTCTC
781GGCTGGAACA GGATGAATAT GCACTGCGCT CTCACAGTCT AGCCAAGAAG GCACAGGATG
841AAGGACTCCT TTCTGATGTG GTACCCTTCA AAGTACCAGG AAAAGATACA GTTACCAAAG
901ATAATGGCAT CCGTCCTTCC TCACTGGAGC AGATGGCCAA ACTAAAACCT GCATTCATCA
961AGCCCTACGG CACAGTGACA GCTGCAAATT CTTCTTTCTT GACTGATGGT GCATCTGCAA
1021TGTTAATCAT GGCGGAGGAA AAGGCTCTGG CCATGGGTTA TAAGCCGAAG GCATATTTGA
1081GGGATTTTAT GTATGTGTCT CAGGATCCAA AAGATCAACT ATTACTTGGA CCAACATATG
1141CTACTCCAAA AGTTCTAGAA AAGGCAGGAT TGACCATGAA TGATATTGAT GCTTTTGAAT
1201TTCATGAAGC TTTCTCGGGT CAGATTTTGG CAAATTTTAA AGCCATGGAT TCTGATTGGT
1261TTGCAGAAAA CTACATGGGT AGAAAAACCA AGGTTGGATT GCCTCCTTTG GAGAAGTTTA
1321ATAACTGGGG TGGATCTCTG TCCCTGGGAC ACCCATTTGG AGCCACTGGC TGCAGGTTGG
1381TCATGGCTGC TGCCAACAGA TTACGGAAAG AAGGAGGCCA GTATGGCTTA GTGGCTGCGT
1441GTGCAGCTGG AGGGCAGGGC CATGCTATGA TAGTGGAAGC TTATCCAAAA TACCCAACTT
1501TCTTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)