Construct: ORF ccsbBroadEn_10457

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017632.1_s300c1, BRDN0000457385
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ITSN2 (50618)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 50618 ITSN2 intersectin 2 NM_019595.4 99.9% 99.8% 1_3delATG;4576A>C
2 human 50618 ITSN2 intersectin 2 XM_024452934.1 99.9% 99.8% 1_3delATG;4576A>C
3 human 50618 ITSN2 intersectin 2 XM_024452935.1 99.2% 99.1% 0_1ins39;4534A>C
4 human 50618 ITSN2 intersectin 2 NM_001348182.2 99.1% 99.1% 1_3delATG;2134_2135ins39;4537A>C
5 human 50618 ITSN2 intersectin 2 NM_006277.3 98.3% 98.2% 1_3delATG;1861_1941del;4657A>C
6 human 50618 ITSN2 intersectin 2 XM_024452930.1 98.3% 98.2% 1_3delATG;1861_1941del;4657A>C
7 human 50618 ITSN2 intersectin 2 XM_024452931.1 98.3% 98.2% 1_3delATG;1861_1941del;4657A>C
8 human 50618 ITSN2 intersectin 2 NM_001348181.2 97.6% 97.5% 0_1ins39;1819_1899del;4615A>C
9 human 50618 ITSN2 intersectin 2 XM_024452932.1 97.6% 97.5% 0_1ins39;1819_1899del;4615A>C
10 human 50618 ITSN2 intersectin 2 XM_024452933.1 97.5% 97.5% (many diffs)
11 human 50618 ITSN2 intersectin 2 XR_002959302.1 84.7% (many diffs)
12 human 50618 ITSN2 intersectin 2 NM_147152.3 71.6% 71.3% (many diffs)
13 human 50618 ITSN2 intersectin 2 NM_001348183.2 69.7% 69.7% 1_3delATG;3495_3496ins1515
14 human 50618 ITSN2 intersectin 2 NM_001348184.2 68.9% 68.9% 0_1ins39;3453_3454ins1515
15 human 50618 ITSN2 intersectin 2 NM_001348186.2 68.9% 68.9% 1_3delATG;2134_2135ins39;3456_3457ins1515
16 human 50618 ITSN2 intersectin 2 XM_024452936.1 67.8% 67.8% 0_1ins39;1819_1899del;3534_3535ins1515
17 human 50618 ITSN2 intersectin 2 NM_001348185.2 67.8% 67.8% (many diffs)
18 human 50618 ITSN2 intersectin 2 XM_024452937.1 55.4% 54.5% (many diffs)
19 mouse 20403 Itsn2 intersectin 2 NM_011365.4 87.1% 91.7% (many diffs)
20 mouse 20403 Itsn2 intersectin 2 NM_001198968.2 85.8% 90.3% (many diffs)
21 mouse 20403 Itsn2 intersectin 2 XM_006515023.3 85.8% 90.3% (many diffs)
22 mouse 20403 Itsn2 intersectin 2 XM_006515024.3 85.8% 90.3% (many diffs)
23 mouse 20403 Itsn2 intersectin 2 XM_006515025.3 85.1% 89.6% (many diffs)
24 mouse 20403 Itsn2 intersectin 2 XM_006515027.3 60.8% 63.2% (many diffs)
25 mouse 20403 Itsn2 intersectin 2 XM_006515026.3 59.8% 62.2% (many diffs)
26 mouse 20403 Itsn2 intersectin 2 NM_001198969.2 34.1% 35% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
5076
ORF length:
5007
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCTCAGTTT CCCACAGCTA TGAATGGAGG GCCAAACATG TGGGCTATTA
121CCTCTGAAGA ACGTACTAAG CATGACAGGC AGTTTGATAA CCTCAAACCT TCAGGAGGTT
181ACATAACAGG TGATCAAGCA CGTAATTTTT TCCTACAATC AGGTCTGCCG GCCCCTGTTT
241TAGCTGAAAT ATGGGCTTTA TCAGACCTAA ACAAGGATGG GAAGATGGAT CAGCAAGAGT
301TCTCCATAGC TATGAAACTC ATCAAACTGA AGCTTCAAGG CCAACAGTTG CCTGTGGTTC
361TCCCTCCTAT TATGAAGCAA CCCCCTATGT TTTCTCCATT AATTTCTGCT CGTTTTGGAA
421TGGGAAGCAT GCCCAATCTG TCCATTCCTC AGCCATTGCC TCCAGCTGCA CCTATAACAT
481CATTGTCTTC TGCGACTTCA GGGACCAACC TTCCTCCCTT AATGATGCCC ACTCCCCTAG
541TGCCTTCTGT TAGCACATCA TCATTACCAA ATGGAACCGC CAGTCTCATT CAGCCTTTAC
601CCATTCCTTA TTCTTCTTCA ACATTGCCTC ATGGGTCATC TTATAGTCTG ATGATGGGAG
661GATTTGGAGG TGCTAGTATA CAGAAAGCGC AGTCTCTGAT TGATTTAGGA TCTAGTAGCT
721CAACTTCCTC GACTGCTTCA CTCTCAGGGA ACTCACCCAA GACTGGGACC TCAGAGTGGG
781CAGTTCCTCA GCCTACAAGA TTAAAATATC GGCAAAAATT TAATACTCTT GACAAAAGTA
841TGAGTGGATA TCTCTCAGGT TTTCAAGCTA GAAATGCCCT TCTTCAGTCA AATCTTTCTC
901AAACTCAGCT GGCTACTATT TGGACTCTGG CTGACGTTGA TGGTGATGGA CAGCTAAAAG
961CAGAAGAGTT TATTCTTGCA ATGCACCTTA CTGACATGGC CAAAGCTGGA CAGCCATTAC
1021CACTGACTTT ACCTCCTGAG CTTGTTCCTC CATCTTTCAG AGGAGGAAAG CAAATTGATT
1081CCATTAATGG AACTCTGCCT TCATATCAGA AAATGCAAGA AGAGGAGCCT CAGAAGAAAT
1141TACCAGTTAC TTTTGAGGAC AAACGGAAAG CCAACTATGA GCGAGGGAAC ATGGAGCTGG
1201AAAAGCGACG CCAAGCCTTG ATGGAGCAGC AACAAAGGGA GGCAGAACGT AAAGCCCAGA
1261AAGAAAAGGA AGAGTGGGAA CGAAAACAGA GAGAATTACA AGAACAAGAA TGGAAGAAAC
1321AACTTGAATT AGAAAAACGC TTAGAGAAGC AACGGGAATT GGAGAGACAA CGAGAGGAAG
1381AAAGGAGAAA AGACATAGAA AGACGAGAGG CAGCAAAACA GGAACTTGAA CGACAACGTC
1441GCTTAGAATG GGAGAGAATT CGGCGACAGG AGCTTCTCAA TCAAAAGAAT AGAGAACAAG
1501AAGAAATTGT CAGGTTAAAC TCTAAAAAGA AGAATCTTCA TCTTGAGTTG GAAGCACTGA
1561ATGGCAAACA TCAGCAGATC TCAGGCAGAC TTCAGGATGT CCGACTCAAA AAGCAAACTC
1621AAAAGACTGA GCTGGAAGTT CTGGATAAGC AGTGTGACTT GGAAATTATG GAAATCAAGC
1681AACTTCAACA GGAACTTCAG GAATATCAGA ATAAGCTTAT CTATCTGGTA CCTGAGAAGC
1741AATTATTAAA TGAAAGAATT AAAAACATGC AGTTCAGTAA CACACCTGAT TCAGGGGTCA
1801GTTTACTTCA TAAAAAATCA TTAGAAAAGG AAGAATTATG CCAAAGACTT AAAGAACAGT
1861TAGATGCTCT TGAAAAAGAA ACTGCATCTA AGCTGTCAGA AATGGATTCT TTTAACAATC
1921AACTAAAGGA ACTGAGAGAA ACCTACAACA CACAGCAGTT AGCCCTTGAA CAGCTTTATA
1981AGATCAAACG TGACAAGTTG AAGGAAATTG AAAGGAAAAG ATTAGAACTA ATGCAGAAAA
2041AGAAACTAGA AGATGAGGCT GCAAGGAAAG CAAAGCAAGG AAAAGAAAAC TTATGGAAAG
2101AAAATCTTAG AAAGGAGGAA GAAGAAAAAC AAAAGCGACT CCAGGAAGAA AAAACACAAG
2161AAAAAATTCA AGAAGAGGAA CGGAAAGCTG AGGAGAAACA ACGTAAGGAT AAGGATACTT
2221TGAAAGCTGA GGAGAAAAAA CGTGAGACAG CTAGTGTTTT GGTGAATTAT AGAGCATTAT
2281ACCCCTTTGA AGCAAGGAAC CATGATGAGA TGAGTTTTAA TTCTGGAGAT ATAATTCAGG
2341TTGATGAAAA AACCGTAGGA GAACCTGGTT GGCTTTATGG TAGTTTTCAA GGAAATTTTG
2401GCTGGTTTCC ATGCAATTAT GTAGAAAAAA TGCCATCAAG TGAAAATGAA AAAGCTGTAT
2461CTCCAAAGAA GGCCTTACTT CCTCCTACAG TTTCTTTATC TGCTACCTCA ACTTCCTCTG
2521AACCACTTTC TTCAAATCAA CCAGCATCAG TGACTGATTA TCAAAATGTA TCTTTTTCAA
2581ACCTAACTGT AAATACATCA TGGCAGAAAA AATCAGCCTT CACTCGAACT GTGTCCCCTG
2641GATCTGTATC ACCTATTCAT GGACAGGGAC AAGTGGTAGA AAACTTAAAA GCACAGGCCC
2701TTTGTTCCTG GACTGCAAAG AAAGATAACC ACTTGAACTT CTCAAAACAT GACATTATTA
2761CTGTCTTGGA GCAGCAAGAA AATTGGTGGT TTGGGGAGGT GCATGGAGGA AGAGGATGGT
2821TTCCCAAATC TTATGTCAAG ATCATTCCTG GGAGTGAAGT AAAACGGGAA GAACCAGAAG
2881CTTTGTATGC AGCTGTAAAT AAGAAACCTA CCTCGGCAGC CTATTCAGTT GGAGAAGAAT
2941ATATTGCACT TTATCCATAT TCAAGTGTGG AACCTGGAGA TTTGACTTTC ACAGAAGGTG
3001AAGAAATATT GGTGACCCAG AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGATAGAA
3061GTGGAATTTT TCCATCAAAC TATGTCAAAC CAAAGGATCA AGAGAGTTTT GGGAGTGCTA
3121GCAAGTCTGG AGCATCAAAT AAAAAACCTG AGATTGCTCA GGTAACTTCA GCATATGTTG
3181CTTCTGGTTC TGAACAACTT AGCCTTGCAC CAGGACAGTT AATATTAATT CTAAAGAAAA
3241ATACAAGTGG GTGGTGGCAA GGAGAGTTAC AGGCCAGAGG AAAAAAGCGA CAGAAAGGAT
3301GGTTTCCTGC CAGTCATGTT AAACTTTTGG GTCCAAGTAG TGAAAGAGCC ACACCTGCCT
3361TTCATCCTGT ATGTCAGGTG ATTGCTATGT ATGACTATGC AGCAAATAAT GAAGATGAGC
3421TCAGTTTCTC CAAGGGACAA CTCATTAATG TTATGAACAA AGATGATCCT GATTGGTGGC
3481AAGGAGAGAT CAACGGGGTG ACTGGTCTCT TTCCTTCAAA CTACGTTAAG ATGACGACAG
3541ACTCAGATCC AAGTCAACAG TGGTGTGCTG ATCTGCAAAC CCTGGACACA ATGCAGCCAA
3601TTGAGAGGAA AAGACAGGGC TATATTCATG AGCTGATTCA GACCGAAGAG CGGTACATGG
3661CTGACCTTCA GCTCGTCGTC GAGGTTTTTC AGAAACGCAT GGCAGAGTCA GGCTTTCTCA
3721CTGAAGGGGA GATGGCCCTG ATTTTTGTTA ACTGGAAGGA GCTCATCATG TCCAACACAA
3781AGCTGCTGAA GGCTTTGCGG GTGCGGAAGA AGACCGGGGG CGAGAAGATG CCGGTGCAGA
3841TGATTGGGGA CATCCTGGCC GCTGAGCTGT CCCACATGCA GGCTTACATC AGGTTCTGCA
3901GCTGCCAGCT TAATGGAGCA GCTCTGTTAC AGCAGAAGAC AGATGAAGAC ACAGATTTCA
3961AAGAATTTTT AAAGAAGCTG GCATCTGACC CGCGGTGTAA AGGAATGCCC CTCTCCAGCT
4021TCCTGCTGAA ACCCATGCAG AGGATCACCC GCTACCCACT GCTCATCAGA AGTATTCTGG
4081AGAACACCCC GGAGAGCCAT GCAGACCATT CCTCCCTAAA GCTGGCCCTC GAGCGGGCAG
4141AGGAGCTGTG CTCTCAAGTG AATGAGGGAG TTCGGGAGAA GGAAAACTCG GACCGACTGG
4201AGTGGATCCA GGCGCACGTG CAGTGTGAAG GCCTCGCGGA GCAACTTATT TTCAACTCTC
4261TCACCAACTG CCTGGGGCCC CGGAAGCTCT TACACAGTGG GAAATTATAC AAGACCAAGA
4321GCAACAAGGA ACTGCACGGA TTCCTCTTCA ATGACTTCCT GCTTCTTACC TACATGGTCA
4381AGCAGTTTGC TGTTTCCTCT GGCTCTGAGA AACTTTTCAG CTCGAAGTCC AATGCTCAAT
4441TCAAAATGTA TAAAACGCCC ATTTTCCTGA ATGAAGTCTT GGTGAAACTG CCCACAGACC
4501CTTCCAGCGA TGAGCCTGTC TTCCACATTT CCCACATTGA TCGGGTCTAC ACCCTCCGAA
4561CAGACAACAT TAATGAGAGG ACCGCCTGGG TGCAGAAGAT CAAGGCGGCG TCTGAGCAGT
4621ACATCGACAC CGAGAAGAAG CAGCGTGAGA AAGCTTACCA AGCCCGCTCC CAAAAGACTT
4681CAGGCATTGG GCGCCTGATG GTGCATGTCA TTGAAGCTAC AGAATTAAAA GCCTGCAAAC
4741CAAATGGAAA GAGCAACCCA TACTGTGAAA TCAGCATGGG CTCCCAGAGC TACACCACCA
4801GGACCATCCA GGACACACTC AATCCCAAGT GGAATTTTAA CTGCCAGTTC TTTATTAAGG
4861ATCTCTACCA AGACGTGCTG TGTCTCACCC TGTTTGACAG AGACCAGTTT TCACCAGATG
4921ATTTCCTGGG TCGTACTGAA ATTCCAGTGG CAAAAATTCG AACAGAACAG GAAAGCAAAG
4981GCCCTATGAC CCGCCGACTG CTGCTGCATG AGGTCCCCAC CGGGGAGGTC TGGGTCCGTT
5041TTGACCTGCA GCTTTTTGAG CAAAAAACTC TCCTGTTGCC AACTTTCTTG TACAAAGTTG
5101Gcattataag aaagcattgc ttatcaattt gttgcaacga ac

Download FASTA (ORF) (Full)