Construct: ORF ccsbBroadEn_10813

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF007077.1_s300c1, BRDN0000456951
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: EYA1 (2138)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	2138	EYA1	EYA transcriptional coactiv...	XM_017013213.1	91.4%	91.5%	0_1ins132;1404T>C
2	human	2138	EYA1	EYA transcriptional coactiv...	XM_017013212.2	91.1%	88.1%	(many diffs)
3	human	2138	EYA1	EYA transcriptional coactiv...	NM_001288575.2	90.4%	90.5%	0_1ins132;66_67insGGTGCATTGTGGGCA;1389T>C
4	human	2138	EYA1	EYA transcriptional coactiv...	XM_017013211.2	89.2%	86.3%	(many diffs)
5	human	2138	EYA1	EYA transcriptional coactiv...	NM_000503.6	86.1%	83.3%	(many diffs)
6	human	2138	EYA1	EYA transcriptional coactiv...	NM_001370334.1	86.1%	83.3%	(many diffs)
7	human	2138	EYA1	EYA transcriptional coactiv...	NM_001370335.1	86.1%	83.3%	(many diffs)
8	human	2138	EYA1	EYA transcriptional coactiv...	NM_172058.4	86.1%	83.3%	(many diffs)
9	human	2138	EYA1	EYA transcriptional coactiv...	XM_011517483.2	85.9%	82.9%	(many diffs)
10	human	2138	EYA1	EYA transcriptional coactiv...	XM_017013208.2	85.9%	82.9%	(many diffs)
11	human	2138	EYA1	EYA transcriptional coactiv...	NM_001288574.2	85.1%	82.1%	(many diffs)
12	human	2138	EYA1	EYA transcriptional coactiv...	NM_001370333.1	82.1%	79.4%	(many diffs)
13	human	2138	EYA1	EYA transcriptional coactiv...	XM_017013202.1	82.1%	79.4%	(many diffs)
14	human	2138	EYA1	EYA transcriptional coactiv...	XM_017013203.2	81.9%	79.1%	(many diffs)
15	human	2138	EYA1	EYA transcriptional coactiv...	XM_011517484.3	81.3%	78.7%	(many diffs)
16	human	2138	EYA1	EYA transcriptional coactiv...	XM_017013204.2	81.1%	78.3%	(many diffs)
17	human	2138	EYA1	EYA transcriptional coactiv...	XM_017013205.2	78.7%	75.7%	(many diffs)
18	human	2138	EYA1	EYA transcriptional coactiv...	XM_017013207.2	77.3%	74.7%	(many diffs)
19	human	2138	EYA1	EYA transcriptional coactiv...	NM_172059.5	76.5%	73.9%	(many diffs)
20	human	2138	EYA1	EYA transcriptional coactiv...	NM_001370336.1	76.3%	73.6%	(many diffs)
21	mouse	14048	Eya1	EYA transcriptional coactiv...	NM_001252192.1	82.8%	85.8%	(many diffs)
22	mouse	14048	Eya1	EYA transcriptional coactiv...	XM_017315414.1	82.4%	89.5%	(many diffs)
23	mouse	14048	Eya1	EYA transcriptional coactiv...	XM_017315422.1	82.4%	89.5%	(many diffs)
24	mouse	14048	Eya1	EYA transcriptional coactiv...	XM_006495452.3	80.5%	78.3%	(many diffs)
25	mouse	14048	Eya1	EYA transcriptional coactiv...	XM_011238346.2	80.5%	78.3%	(many diffs)
26	mouse	14048	Eya1	EYA transcriptional coactiv...	XM_017315381.1	80.5%	78.3%	(many diffs)
27	mouse	14048	Eya1	EYA transcriptional coactiv...	XM_011238344.2	78.3%	81.4%	(many diffs)
28	mouse	14048	Eya1	EYA transcriptional coactiv...	NM_001310459.1	78.2%	81.1%	(many diffs)
29	mouse	14048	Eya1	EYA transcriptional coactiv...	XM_017315348.1	78.2%	81.1%	(many diffs)
30	mouse	14048	Eya1	EYA transcriptional coactiv...	NM_010164.2	77.5%	80.6%	(many diffs)
31	mouse	14048	Eya1	EYA transcriptional coactiv...	XM_006495451.3	77.4%	80.3%	(many diffs)
32	mouse	14048	Eya1	EYA transcriptional coactiv...	XM_006495446.3	76.1%	74.2%	(many diffs)
33	mouse	14048	Eya1	EYA transcriptional coactiv...	XM_011238347.2	63.4%	65.1%	(many diffs)
34	mouse	14048	Eya1	EYA transcriptional coactiv...	XR_001780756.1	49%		(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

69

ORF end:

1626

ORF length:

1557

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61	TTGGCACCAT GTTTCCATCT AATGCATGGG CTTTCTATTT TCTGTCATTT CTGACAAATA
121	GCAGACCATA CCCACATATT CTCCCTACCC CTTCCTCACA AACTATGGCT GCATATGGGC
181	AAACACAGTT TACCACAGGA ATGCAACAAG CTACAGCCTA TGCCACGTAC CCACAGCCAG
241	GACAGCCGTA CGGCATTTCC TCATATGGTG CATTGTGGGC AGGCATCAAG ACTGAAGGTG
301	GATTGTCACA GTCTCAGTCA CCTGGACAGA CAGGATTTCT CAGCTATGGC ACAAGCTTCA
361	GTACCCCTCA ACCTGGACAG GCACCATACA GCTACCAGAT GCAAGGTAGC AGTTTTACAA
421	CATCATCAGG AATATATACA GGAAATAATT CACTCACAAA TTCCTCTGGA TTTAATAGTT
481	CACAGCAGGA CTATCCGTCT TATCCCAGTT TTGGCCAGGG TCAGTACGCA CAGTATTATA
541	ACAGCTCACC GTATCCAGCA CATTATATGA CCAGCAGCAA CACCAGCCCA ACGACACCAT
601	CCACCAATGC CACTTACCAG CTTCAAGAAC CGCCATCTGG CATCACCAGC CAAGCAGTTA
661	CAGATCCCAC AGCAGAGTAC AGCACAATCC ACAGCCCATC AACACCCATT AAAGATTCAG
721	ATTCTGATCG ATTGCGTCGA GGTTCAGATG GGAAATCACG TGGACGGGGC CGAAGAAACA
781	ATAATCCTTC ACCTCCCCCA GATTCTGATC TTGAGAGAGT GTTCATCTGG GACTTGGATG
841	AGACAATCAT TGTTTTCCAC TCCTTGCTTA CTGGGTCCTA CGCCAACAGA TATGGGAGGG
901	ATCCACCCAC TTCAGTTTCC CTTGGACTGC GAATGGAAGA AATGATTTTC AACTTGGCAG
961	ACACACATTT ATTTTTTAAT GACTTAGAAG AATGTGACCA AGTCCATATA GATGATGTTT
1021	CTTCAGATGA TAACGGACAG GACCTAAGCA CATATAACTT TGGAACAGAT GGCTTTCCTG
1081	CTGCAGCAAC CAGTGCTAAC TTATGTTTGG CAACTGGTGT ACGGGGCGGT GTGGACTGGA
1141	TGAGAAAGTT GGCCTTCCGC TACAGACGGG TAAAAGAGAT CTACAACACC TACAAAAATA
1201	ATGTTGGAGG TCTGCTTGGT CCAGCTAAGA GGGAAGCCTG GCTGCAGTTG AGGGCCGAAA
1261	TTGAAGCCCT GACCGACTCC TGGTTGACAC TGGCCCTGAA AGCACTCTCG CTCATTCACT
1321	CCCGGACAAA CTGTGTGAAT ATTTTAGTAA CAACTACTCA GCTCATCCCA GCATTGGCGA
1381	AAGTCCTGCT GTATGGGTTA GGAATTGTAT TTCCAATAGA AAATATTTAC AGTGCAACTA
1441	AAATAGGAAA AGAAAGCTGT TTTGAGAGAA TAATTCAAAG GTTTGGAAGA AAAGTGGTGT
1501	ATGTTGTTAT AGGAGATGGT GTAGAAGAAG AACAAGGAGC AAAAAAGCAC GCGATGCCCT
1561	TCTGGAGGAT CTCCAGCCAC TCGGACCTCA TGGCCCTGCA CCACGCCTTG GAACTGGAGT
1621	ACCTGTTGCC AACTTTCTTG TACAAAGTTG Gcattataag aaagcattgc ttatcaattt
1681	gttgcaacga ac

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