Construct: ORF ccsbBroadEn_10823

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010650.1_s300c1, BRDN0000388996
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FLOT2 (2319)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2319 FLOT2 flotillin 2 XM_017024394.1 100% 100%
2 human 2319 FLOT2 flotillin 2 XM_017024395.1 100% 100%
3 human 2319 FLOT2 flotillin 2 XM_017024396.1 97.2% 97.8% (many diffs)
4 human 2319 FLOT2 flotillin 2 XM_005257953.2 92.6% 92.6% 0_1ins84
5 human 2319 FLOT2 flotillin 2 XM_024450667.1 92.6% 92.6% 0_1ins84
6 human 2319 FLOT2 flotillin 2 NM_004475.3 88.5% 88.5% 1_147del
7 human 2319 FLOT2 flotillin 2 NM_001330170.2 86.1% 86.6% (many diffs)
8 human 2319 FLOT2 flotillin 2 XM_024450666.1 82.2% 82.2% 1_147del;579_677del
9 mouse 14252 Flot2 flotillin 2 NM_001284228.1 90.4% 99.2% (many diffs)
10 mouse 14252 Flot2 flotillin 2 NM_008028.2 90.4% 99.2% (many diffs)
11 mouse 14252 Flot2 flotillin 2 XM_006532192.2 90.4% 99.2% (many diffs)
12 mouse 14252 Flot2 flotillin 2 XM_006532193.3 90.4% 99.2% (many diffs)
13 mouse 14252 Flot2 flotillin 2 XM_006532194.1 83.1% 91.8% (many diffs)
14 mouse 14252 Flot2 flotillin 2 NM_001040403.1 80% 87.8% (many diffs)
15 mouse 14252 Flot2 flotillin 2 NM_001284227.1 75.1% 82.4% (many diffs)
16 mouse 14252 Flot2 flotillin 2 XM_006532191.3 67.5% 74.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1203
ORF length:
1137
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAC GTTGCAGCCC CGCTGCGAGG ACGTAGAGAC GGCCGAGGGG GTAGCTTTAA
121CTGTGACGGG TGTCGCCCAG GTGAAGATCA TGACGGAGAA GGAACTCCTG GCCGTGGCTT
181GTGAGCAGTT TCTGGGTAAG AATGTGCAGG ACATCAAAAA CGTCGTCCTG CAGACCCTGG
241AGGGACATCT GCGCTCCATC CTCGGGACCC TGACAGTGGA GCAGATTTAT CAGGACCGGG
301ACCAGTTTGC CAAGCTGGTG CGGGAGGTGG CAGCCCCTGA TGTTGGCCGC ATGGGCATTG
361AGATCCTCAG CTTCACCATC AAGGACGTGT ATGACAAAGT GGACTATCTG AGCTCCCTGG
421GCAAGACGCA GACTGCCGTG GTGCAGAGAG ATGCTGACAT TGGCGTGGCC GAGGCTGAAC
481GGGACGCAGG CATCCGGGAA GCTGAGTGCA AGAAGGAGAT GCTGGATGTG AAGTTCATGG
541CAGACACCAA GATTGCTGAC TCTAAGCGAG CCTTCGAGCT GCAAAAGTCA GCCTTCAGTG
601AGGAGGTTAA CATCAAGACA GCTGAGGCCC AGTTGGCCTA TGAGCTGCAG GGGGCCCGTG
661AACAGCAGAA GATCCGGCAG GAAGAGATTG AGATTGAGGT TGTGCAGCGC AAGAAACAGA
721TTGCCGTGGA GGCACAGGAG ATCCTGCGTA CGGACAAGGA GCTCATCGCT ACAGTGCGCC
781GGCCTGCCGA GGCCGAGGCC CACCGCATCC AGCAGATTGC CGAGGGTGAA AAGGTGAAGC
841AGGTCCTCTT GGCACAGGCA GAGGCTGAGA AGATCCGCAA AATCGGGGAG GCGGAAGCGG
901CAGTCATCGA GGCGATGGGC AAGGCAGAGG CTGAGCGGAT GAAGCTCAAG GCAGAAGCCT
961ACCAGAAATA CGGGGATGCA GCCAAGATGG CCTTGGTGCT AGAGGCCCTG CCCCAGATTG
1021CTGCCAAAAT CGCTGCCCCA CTTACCAAGG TCGATGAGAT TGTGGTCCTC AGTGGAGACA
1081ACAGTAAGGT CACATCAGAA GTGAACCGAC TGCTGGCCGA GCTGCCTGCC TCTGTGCATG
1141CCCTCACAGG CGTGGACCTG TCTAAGATAC CCCTGATCAA GAAGGCCACT GGTGTGCAGG
1201TGTGCCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt
1261gcaacgaac

Download FASTA (ORF) (Full)