Transcript: Human XM_005257953.2

PREDICTED: Homo sapiens flotillin 2 (FLOT2), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FLOT2 (2319)
Length:
2567
CDS:
290..1345

Additional Resources:

NCBI RefSeq record:
XM_005257953.2
NBCI Gene record:
FLOT2 (2319)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005257953.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000382061 TGTTGTGGTTCCGACTATAAA pLKO_005 204 5UTR 100% 15.000 21.000 N FLOT2 n/a
2 TRCN0000149396 GAGCAGTTTCTGGGTAAGAAT pLKO.1 323 CDS 100% 5.625 3.938 N FLOT2 n/a
3 TRCN0000280591 GAGCAGTTTCTGGGTAAGAAT pLKO_005 323 CDS 100% 5.625 3.938 N FLOT2 n/a
4 TRCN0000146367 GAGACAACAGTAAGGTCACAT pLKO.1 1215 CDS 100% 4.950 3.465 N FLOT2 n/a
5 TRCN0000280590 GAGACAACAGTAAGGTCACAT pLKO_005 1215 CDS 100% 4.950 3.465 N FLOT2 n/a
6 TRCN0000148873 CGTGTATGACAAAGTGGACTA pLKO.1 526 CDS 100% 4.050 2.835 N FLOT2 n/a
7 TRCN0000280655 CGTGTATGACAAAGTGGACTA pLKO_005 526 CDS 100% 4.050 2.835 N FLOT2 n/a
8 TRCN0000150223 GAAGAGATTGAGATTGAGGTT pLKO.1 821 CDS 100% 2.640 1.848 N FLOT2 n/a
9 TRCN0000280654 GAAGAGATTGAGATTGAGGTT pLKO_005 821 CDS 100% 2.640 1.848 N FLOT2 n/a
10 TRCN0000380909 CCCACTTTCACTGCCACTTTC pLKO_005 1543 3UTR 100% 10.800 6.480 N FLOT2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005257953.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10823 pDONR223 100% 92.6% 92.6% None 0_1ins84 n/a
2 ccsbBroad304_10823 pLX_304 0% 92.6% 92.6% V5 0_1ins84 n/a
3 TRCN0000472011 CTGCAACTGTTAATACCTCTGATA pLX_317 12.4% 92.6% 92.6% V5 0_1ins84 n/a
4 ccsbBroadEn_10824 pDONR223 100% 70.8% 70.2% None (many diffs) n/a
5 ccsbBroad304_10824 pLX_304 0% 70.8% 70.2% V5 (many diffs) n/a
6 TRCN0000468885 GTGTAATATTCACATTTTTAGTGC pLX_317 9.3% 70.8% 70.2% V5 (many diffs) n/a
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