Transcript: Mouse XM_006532194.1

PREDICTED: Mus musculus flotillin 2 (Flot2), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Flot2 (14252)
Length:
2501
CDS:
245..1300

Additional Resources:

NCBI RefSeq record:
XM_006532194.1
NBCI Gene record:
Flot2 (14252)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006532194.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380501 TGACTAAAGTCGATGAGATTG pLKO_005 1137 CDS 100% 10.800 15.120 N Flot2 n/a
2 TRCN0000109502 CTGACTAAAGTCGATGAGATT pLKO.1 1136 CDS 100% 4.950 6.930 N Flot2 n/a
3 TRCN0000304869 CTGACTGTAGAACAGATTTAT pLKO_005 365 CDS 100% 15.000 10.500 N Flot2 n/a
4 TRCN0000380359 ACAGGGACAGCGAGAACATTT pLKO_005 1360 3UTR 100% 13.200 9.240 N Flot2 n/a
5 TRCN0000304812 CAAGGTGACATCAGAAGTAAA pLKO_005 1180 CDS 100% 13.200 9.240 N Flot2 n/a
6 TRCN0000381690 ACCAAGATCGCTGACTCTAAG pLKO_005 641 CDS 100% 10.800 7.560 N Flot2 n/a
7 TRCN0000109500 GCCTTCAAGTTCTACATGTAT pLKO.1 1676 3UTR 100% 5.625 3.938 N Flot2 n/a
8 TRCN0000109503 GCTTCACCATCAAGGATGTTT pLKO.1 465 CDS 100% 5.625 3.938 N Flot2 n/a
9 TRCN0000316514 GCTTCACCATCAAGGATGTTT pLKO_005 465 CDS 100% 5.625 3.938 N Flot2 n/a
10 TRCN0000109504 GAAGCTGAGAAGATTCGCAAA pLKO.1 956 CDS 100% 4.050 2.835 N Flot2 n/a
11 TRCN0000316525 GAAGCTGAGAAGATTCGCAAA pLKO_005 956 CDS 100% 4.050 2.835 N Flot2 n/a
12 TRCN0000109501 GCTGACTCTAAGAGAGCCTTT pLKO.1 650 CDS 100% 4.050 2.835 N Flot2 n/a
13 TRCN0000304813 CTCCCATGCCCTCACATTAAT pLKO_005 1474 3UTR 100% 15.000 9.000 N Flot2 n/a
14 TRCN0000150223 GAAGAGATTGAGATTGAGGTT pLKO.1 776 CDS 100% 2.640 1.848 N FLOT2 n/a
15 TRCN0000280654 GAAGAGATTGAGATTGAGGTT pLKO_005 776 CDS 100% 2.640 1.848 N FLOT2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006532194.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10823 pDONR223 100% 83.1% 91.8% None (many diffs) n/a
2 ccsbBroad304_10823 pLX_304 0% 83.1% 91.8% V5 (many diffs) n/a
3 TRCN0000472011 CTGCAACTGTTAATACCTCTGATA pLX_317 12.4% 83.1% 91.8% V5 (many diffs) n/a
4 ccsbBroadEn_10824 pDONR223 100% 64.6% 70% None (many diffs) n/a
5 ccsbBroad304_10824 pLX_304 0% 64.6% 70% V5 (many diffs) n/a
6 TRCN0000468885 GTGTAATATTCACATTTTTAGTGC pLX_317 9.3% 64.6% 70% V5 (many diffs) n/a
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