Construct: ORF ccsbBroadEn_11042

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003368.1_s300c1, BRDN0000397576
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
POLE (5426)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5426 POLE DNA polymerase epsilon, cat... XM_011534802.3 26% 24.2% (many diffs)
2 human 5426 POLE DNA polymerase epsilon, cat... XM_011534797.3 17% 15.8% (many diffs)
3 human 5426 POLE DNA polymerase epsilon, cat... NM_006231.4 16.1% 16.1% 1_5748del
4 human 5426 POLE DNA polymerase epsilon, cat... XM_011534795.3 14.7% 13.7% (many diffs)
5 human 5426 POLE DNA polymerase epsilon, cat... XR_002957339.1 12.7% 1_6294del;7076_7421del;7751_8692del
6 mouse 18973 Pole polymerase (DNA directed), ... XM_017320715.1 14.1% 14% (many diffs)
7 mouse 18973 Pole polymerase (DNA directed), ... NM_011132.2 13.5% 13.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1176
ORF length:
1110
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA TCCATCTAAC TATGGCGGAA TCAAAGGAAA AGTTTCATCT CGTATTCACT
121GTGGACTGCA AGACTCCCAG AAAGCAGGGG GAGCAGAGGA TGAGCAGGAA AATGAGGACG
181ATGAGGAGGA AAGAGATGGG GAGGAGGAGG AAGAGGCGGA GGAATCCAAC GTGGAGGATT
241TACTGGAAAA CAACTGGAAC ATTTTGCAGT TTTTGCCACA GGCAGCCTCC TGCCAGAACT
301ACTTCCTCAT GATTGTTTCA GCGTACATCG TGGCCGTGTA CCACTGCATG AAGGACGGGC
361TGAGGCGCAG TGCTCCAGGG AGCACCCCCG TGAGGAGGAG GGGGGCCAGC CAGCTCTCCC
421AGGAGGCCGA GGGGGCGGTC GGAGCCCTTC CCGGAATGAT CACCTTCTCT CAGGATTATG
481TCGCAAATGA GCTCACTCAG AGCTTCTTCA CCATCACTCA GAAGATTCAG AAGAAAGTCA
541CAGGCTCTCG GAACTCCACT GAGCTCTCAG AGATGTTTCC TGTCCTCCCC GGTTCCCACT
601TGCTGCTCAA TAACCCTGCC CTGGAGTTCA TCAAATACGT GTGCAAGGTG CTGTCCCTGG
661ACACCAACAT CACAAACCAG GTGAATAAGC TGAACCGAGA CCTGCTTCGC CTGGTGGATG
721TCGGCGAGTT CTCCGAGGAG GCCCAGTTCC GAGACCCCTG CCGCTCCTAC GTGCTTCCTG
781AGGTCATCTG CCGCAGCTGT AACTTCTGCC GCGACCTGGA CCTGTGTAAA GACTCTTCCT
841TCTCAGAGGA TGGGGCGGTC CTGCCTCAGT GGCTCTGCTC CAACTGTCAG GCGCCCTACG
901ACTCCTCTGC CATCGAGATG ACGCTGGTGG AAGTTCTACA GAAGAAGCTG ATGGCCTTCA
961CCCTGCAGGA CCTGGTCTGC CTGAAGTGCC GCGGGGTGAA GGAGACCAGC ATGCCTGTGT
1021ACTGCAGCTG CGCGGGAGAC TTCGCCCTCA CCATCCACAC CCAGGTCTTC ATGGAACAGA
1081TCGGAATATT CCGGAACATT GCCCAGCACT ACGGCATGTC GTACCTCCTG GAGACCCTGG
1141AGTGGCTGCT GCAGAAGAAC CCACAGCTGG GCCATTACCC AACTTTCTTG TACAAAGTtg
1201gcattataag aaagcattgc ttatcaattt gttgcaacga ac

Download FASTA (ORF) (Full)