Transcript: Human XR_002957339.1

PREDICTED: Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X5, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
POLE (5426)
Length:
8692
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002957339.1
NBCI Gene record:
POLE (5426)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002957339.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000052973 GCAGTGGATTACTACTTTATT pLKO.1 445 3UTR 100% 15.000 10.500 N POLE n/a
2 TRCN0000052974 CGCTCCAACATGGTCTACAAT pLKO.1 4372 3UTR 100% 5.625 3.938 N POLE n/a
3 TRCN0000052975 GCTGACCAGTTGGACAACATT pLKO.1 838 3UTR 100% 5.625 3.938 N POLE n/a
4 TRCN0000052977 GCCAAATCACTTGGTGGGTTT pLKO.1 627 3UTR 100% 4.050 2.835 N POLE n/a
5 TRCN0000052976 CCTGTGTAAAGACTCTTCCTT pLKO.1 7050 3UTR 100% 3.000 1.800 N POLE n/a
6 TRCN0000120311 CGCCATATCTACCTGTACCAT pLKO.1 4657 3UTR 100% 3.000 2.100 N Pole n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002957339.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11042 pDONR223 100% 12.7% None 1_6294del;7076_7421del;7751_8692del n/a
2 ccsbBroad304_11042 pLX_304 0% 12.7% V5 1_6294del;7076_7421del;7751_8692del n/a
3 TRCN0000466721 CGACTGACGATCACGCATCGCGTC pLX_317 25.6% 12.7% V5 1_6294del;7076_7421del;7751_8692del n/a
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