Construct: ORF ccsbBroadEn_11086

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008311.1_s300c1, BRDN0000455603
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RAD51 (5888)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5888 RAD51 RAD51 recombinase XM_011521862.3 80.6% 70.3% (many diffs)
2 human 5888 RAD51 RAD51 recombinase NM_002875.5 71.3% 71.3% 223_513del
3 human 5888 RAD51 RAD51 recombinase XM_006720626.3 71.3% 71.3% 223_513del
4 human 5888 RAD51 RAD51 recombinase XM_011521857.2 71.3% 71.3% 223_513del
5 human 5888 RAD51 RAD51 recombinase XM_011521858.2 71.3% 71.3% 223_513del
6 human 5888 RAD51 RAD51 recombinase XM_011521859.2 71.3% 71.3% 223_513del
7 human 5888 RAD51 RAD51 recombinase XM_011521860.2 71.3% 71.3% 223_513del
8 human 5888 RAD51 RAD51 recombinase NM_001164269.2 71.1% 71.1% 223_516del
9 human 5888 RAD51 RAD51 recombinase NM_133487.4 71.1% 71.1% 223_516del
10 human 5888 RAD51 RAD51 recombinase NM_001164270.2 53.9% 45.7% 223_513del;772_773ins122;840_841ins55
11 human 5888 RAD51 RAD51 recombinase XM_011521861.2 53.9% 45.7% 223_513del;772_773ins122;840_841ins55
12 mouse 19361 Rad51 RAD51 recombinase NM_011234.4 65% 70.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
792
ORF length:
726
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC AATGCAGATG CAGCTTGAAG CAAATGCAGA TACTTCAGTG GAAGAAGAAA
121GCTTTGGCCC ACAACCCATT TCACGGTTAG AGCAGTGTGG CATAAATGCC AACGATGTGA
181AGAAATTGGA AGAAGCTGGA TTCCATACTG TGGAGGCTGT TGCCTATGCG CCAAAGAAGG
241AGCTAATAAA TATTAAGGGA ATTAGTGAAG CCAAAGCTGA TAAAATTCTG GCAGTGGCTG
301AGAGGTATGG TCTCTCTGGC AGTGATGTCC TGGATAATGT AGCATATGCT CGAGCGTTCA
361ACACAGACCA CCAGACCCAG CTCCTTTATC AAGCATCAGC CATGATGGTA GAATCTAGGT
421ATGCACTGCT TATTGTAGAC AGTGCCACCG CCCTTTACAG AACAGACTAC TCGGGTCGAG
481GTGAGCTTTC AGCCAGGCAG ATGCACTTGG CCAGGTTTCT GCGGATGCTT CTGCGACTCG
541CTGATGAGTT TGGTGTAGCA GTGGTAATCA CTAATCAGGT GGTAGCTCAA GTGGATGGAG
601CAGCGATGTT TGCTGCTGAT CCCAAAAAAC CTATTGGAGG AAATATCATC GCCCATGCAT
661CAACAACCAG ATTGTATCTG AGGAAAGGAA GAGGGGAAAC CAGAATCTGC AAAATCTACG
721ACTCTCCCTG TCTTCCTGAA GCTGAAGCTA TGTTCGCCAT TAATGCAGAT GGAGTGGGAG
781ATGCCAAAGA CTGCCCAACT TTCTTGTACA AAGTTGGcat tataagaaag cattgcttat
841caatttgttg caacgaac

Download FASTA (ORF) (Full)