Transcript: Human NM_001164269.2

Homo sapiens RAD51 recombinase (RAD51), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RAD51 (5888)
Length:
2335
CDS:
151..1173

Additional Resources:

NCBI RefSeq record:
NM_001164269.2
NBCI Gene record:
RAD51 (5888)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001164269.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000329688 TTAGAGCAGTGTGGCATAAAT pLKO_005 232 CDS 100% 15.000 21.000 N RAD51 n/a
2 TRCN0000018878 CCACAACCCATTTCACGGTTA pLKO.1 214 CDS 100% 4.050 5.670 N RAD51 n/a
3 TRCN0000018875 GCTAAGACTAACTCAAGATAA pLKO.1 1749 3UTR 100% 13.200 9.240 N RAD51 n/a
4 TRCN0000018876 CGCCCTTTACAGAACAGACTA pLKO.1 828 CDS 100% 4.950 3.465 N RAD51 n/a
5 TRCN0000329687 CGCCCTTTACAGAACAGACTA pLKO_005 828 CDS 100% 4.950 3.465 N RAD51 n/a
6 TRCN0000018877 GCTGAAGCTATGTTCGCCATT pLKO.1 1120 CDS 100% 4.050 2.835 N RAD51 n/a
7 TRCN0000353568 GCTGAAGCTATGTTCGCCATT pLKO_005 1120 CDS 100% 4.050 2.835 N RAD51 n/a
8 TRCN0000130146 CAGGTTCAAGTGATTCTCCTA pLKO.1 446 CDS 100% 2.640 1.320 Y DICER1-AS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001164269.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11086 pDONR223 100% 71.1% 71.1% None 223_516del n/a
2 ccsbBroad304_11086 pLX_304 0% 71.1% 71.1% V5 223_516del n/a
3 TRCN0000475891 GTATCTATCATTCAAGGGACCTAG pLX_317 49.3% 71.1% 71.1% V5 223_516del n/a
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