Construct: ORF ccsbBroadEn_11130

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010057.1_s300c1, BRDN0000386875
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SH3GL3 (6457)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6457 SH3GL3 SH3 domain containing GRB2 ... NM_003027.5 81.9% 80.8% (many diffs)
2 human 6457 SH3GL3 SH3 domain containing GRB2 ... XM_024450017.1 79.5% 73.6% 624_625ins104;687_688ins73
3 human 6457 SH3GL3 SH3 domain containing GRB2 ... NM_001324182.1 79.2% 77.5% (many diffs)
4 human 6457 SH3GL3 SH3 domain containing GRB2 ... NM_001301109.1 78% 75.3% (many diffs)
5 human 6457 SH3GL3 SH3 domain containing GRB2 ... NM_001324183.1 78% 75.3% (many diffs)
6 human 6457 SH3GL3 SH3 domain containing GRB2 ... XM_011521889.1 78% 75.3% (many diffs)
7 human 6457 SH3GL3 SH3 domain containing GRB2 ... XM_011521891.2 74.8% 67.2% (many diffs)
8 human 6457 SH3GL3 SH3 domain containing GRB2 ... NM_001324184.1 71.8% 70.7% (many diffs)
9 human 6457 SH3GL3 SH3 domain containing GRB2 ... NM_001324186.1 71.8% 70.7% (many diffs)
10 human 6457 SH3GL3 SH3 domain containing GRB2 ... XM_011521892.2 67.9% 65.8% (many diffs)
11 human 6457 SH3GL3 SH3 domain containing GRB2 ... NM_001301108.1 62% 61% (many diffs)
12 human 6457 SH3GL3 SH3 domain containing GRB2 ... NM_001324185.1 62% 61% (many diffs)
13 human 6457 SH3GL3 SH3 domain containing GRB2 ... NM_001324187.1 62% 61% (many diffs)
14 human 6457 SH3GL3 SH3 domain containing GRB2 ... XM_017022486.2 62% 61% (many diffs)
15 human 6457 SH3GL3 SH3 domain containing GRB2 ... XR_001751373.1 47.3% (many diffs)
16 human 6457 SH3GL3 SH3 domain containing GRB2 ... NR_136714.1 43.1% (many diffs)
17 human 6457 SH3GL3 SH3 domain containing GRB2 ... XR_931880.1 42.9% 1_411del;454_604del;1427_2010del
18 human 6457 SH3GL3 SH3 domain containing GRB2 ... NR_136713.1 42.7% 1_306del;490_491insCAGCATA;1164_1996del
19 human 6457 SH3GL3 SH3 domain containing GRB2 ... XR_931879.2 42.4% 1_411del;454_604del;1427_2037del
20 human 6457 SH3GL3 SH3 domain containing GRB2 ... NR_125370.1 42.3% 1_306del;1171_2040del
21 human 6457 SH3GL3 SH3 domain containing GRB2 ... XR_001751372.1 42.3% 1_411del;454_604del;1427_2041del
22 human 6457 SH3GL3 SH3 domain containing GRB2 ... XR_001751375.1 41.4% (many diffs)
23 human 6457 SH3GL3 SH3 domain containing GRB2 ... XR_001751374.2 40.5% (many diffs)
24 human 6457 SH3GL3 SH3 domain containing GRB2 ... NR_136711.1 39.7% (many diffs)
25 human 6457 SH3GL3 SH3 domain containing GRB2 ... XR_931878.2 38.2% 1_411del;454_604del;1427_2259del
26 human 6457 SH3GL3 SH3 domain containing GRB2 ... XR_002957669.1 37.3% (many diffs)
27 human 6457 SH3GL3 SH3 domain containing GRB2 ... NR_136712.1 37% 1_306del;350_680del;1502_2334del
28 human 6457 SH3GL3 SH3 domain containing GRB2 ... XR_931882.2 33.6% (many diffs)
29 mouse 20408 Sh3gl3 SH3-domain GRB2-like 3 NM_017400.6 71% 69.3% (many diffs)
30 mouse 20408 Sh3gl3 SH3-domain GRB2-like 3 NM_001277954.1 53.4% 51.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
930
ORF length:
864
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTC GGTGGCCGGG CTGAAGAAGC AGTTCCACAA AGCCAGCCAG CTATTTAGTG
121AAAAAATAAG TGGTGCTGAA GGAACTAAAC TAGACGATGA ATTTCTTGAC ATGGAAAGGA
181AAATAGATGT TACCAATAAA GTTGTTGCAG AAATTCTTTC AAAAACCACT GAATATCTTC
241AGCCAAATCC AGCATACAGA GCTAAGCTAG GAATGCTGAA CACTGTGTCG AAGATCCGAG
301GGCAGGTGAA GACCACAGGA TACCCGCAGA CGGAAGGCTT GCTGGGGGAC TGTATGCTGA
361AATACGGGAA GGAGCTCGGG GAAGACTCCA CCTTTGGCAA TGCATTGATA GAAGTTGGTG
421AATCCATGAA GCTAATGGCT GAGGTGAAAG ACTCTCTTGA TATTAATGTA AAGCAAACTT
481TTATTGATCC ACTTCAGTTA CTACAAGATA AAGATTTAAA AGAGATCGGG CATCACCTGA
541AAAAGCTGGA AGGCCGCCGC CTGGATTACG ATTATAAAAA GAAACGAGTA GGTAAGATAC
601CAGACGAAGA AGTCAGACAA GCGGTAGAAA AATTTGAAGA GTCAAAGGAG TTGGCTGAAA
661GAAGCATGTT TAACTTTTTA GAAAATGATG TAGAACAAGT CAGCCAGTTG GCTGTGTTCA
721TAGAGGCAGC ATTAGACTAT CACAGACAGT CCACAGAGAT TCTGCAGGAG CTGCAGAGCA
781AGCTACAGAT GCGAATATCA GCTGCATCCA GTGTCCCCAG ACGAGAATAC AAGCCAAGGC
841CTGTGAAAAG GAGTTCTAGT GAGCTCAATG GAGTTTCCAC CACCTCTGTA GTGAAGACGA
901CAGCCTACAG CAGACTGGAA CCAGCAGATT ACCCAACTTT CTTGTACAAA GTtggcatta
961taagaaagca ttgcttatca atttgttgca acgaac

Download FASTA (ORF) (Full)