Construct: ORF ccsbBroadEn_11418

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF000285.1_s300c1, BRDN0000386556
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: GIT2 (9815)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	9815	GIT2	GIT ArfGAP 2	NM_057170.5	86.3%	86.3%	1_258del
2	human	9815	GIT2	GIT ArfGAP 2	XM_006719712.4	80.8%	80.8%	1_258del;1238_1239ins105
3	human	9815	GIT2	GIT ArfGAP 2	NM_014776.5	78.3%	76.1%	(many diffs)
4	human	9815	GIT2	GIT ArfGAP 2	NM_001135213.3	78%	75.7%	(many diffs)
5	human	9815	GIT2	GIT ArfGAP 2	XM_006719708.4	76.3%	76.3%	1_258del;1348_1596del
6	human	9815	GIT2	GIT ArfGAP 2	XM_017020258.2	76.1%	76.1%	1_258del;761_762insCAG;1345_1593del
7	human	9815	GIT2	GIT ArfGAP 2	XM_006719709.4	75.3%	73.6%	(many diffs)
8	human	9815	GIT2	GIT ArfGAP 2	NM_001330153.2	75.2%	73.4%	(many diffs)
9	human	9815	GIT2	GIT ArfGAP 2	NM_001135214.3	74.7%	74.7%	1_258del;1348_1641del
10	human	9815	GIT2	GIT ArfGAP 2	XM_006719707.4	73.2%	73.2%	1_258del;1347_1685del
11	human	9815	GIT2	GIT ArfGAP 2	NM_057169.5	71.8%	71.8%	1_258del;1347_1730del
12	human	9815	GIT2	GIT ArfGAP 2	XM_005253997.4	71.6%	71.6%	1_258del;761_762insCAG;1344_1727del
13	human	9815	GIT2	GIT ArfGAP 2	XM_017020260.2	65.5%	65.4%	(many diffs)
14	human	9815	GIT2	GIT ArfGAP 2	XM_017020259.2	62.9%	62.8%	(many diffs)
15	human	9815	GIT2	GIT ArfGAP 2	NM_139201.3	60%	56.9%	(many diffs)
16	human	9815	GIT2	GIT ArfGAP 2	XM_017020261.2	59.8%	56.8%	(many diffs)
17	human	9815	GIT2	GIT ArfGAP 2	XM_006719713.4	58%	57.8%	(many diffs)
18	human	9815	GIT2	GIT ArfGAP 2	NM_001330154.2	52.9%	52.2%	(many diffs)
19	mouse	26431	Git2	G protein-coupled receptor ...	NM_001077360.1	69.1%	70.9%	(many diffs)
20	mouse	26431	Git2	G protein-coupled receptor ...	NM_001077359.1	68.9%	70.5%	(many diffs)
21	mouse	26431	Git2	G protein-coupled receptor ...	XM_006530338.3	68.9%	70.6%	(many diffs)
22	mouse	26431	Git2	G protein-coupled receptor ...	XM_017320893.1	66.3%	68.1%	(many diffs)
23	mouse	26431	Git2	G protein-coupled receptor ...	NM_019834.3	66.1%	67.9%	(many diffs)
24	mouse	26431	Git2	G protein-coupled receptor ...	XM_006530337.3	66.1%	67.7%	(many diffs)
25	mouse	26431	Git2	G protein-coupled receptor ...	XM_017320892.1	66.1%	67.7%	(many diffs)
26	mouse	26431	Git2	G protein-coupled receptor ...	XM_006530336.3	65.5%	69.5%	(many diffs)
27	mouse	26431	Git2	G protein-coupled receptor ...	XM_006530335.3	65.3%	69.2%	(many diffs)
28	mouse	26431	Git2	G protein-coupled receptor ...	XM_006530334.3	64%	67.8%	(many diffs)
29	mouse	26431	Git2	G protein-coupled receptor ...	NM_001347400.1	62.9%	66.7%	(many diffs)
30	mouse	26431	Git2	G protein-coupled receptor ...	XM_006530333.3	62.8%	66.6%	(many diffs)
31	mouse	26431	Git2	G protein-coupled receptor ...	XM_006530339.3	62.8%	66.4%	(many diffs)
32	mouse	26431	Git2	G protein-coupled receptor ...	XM_006530331.3	62.8%	66.4%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

1701

ORF length:

1635

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61	TTGGCATGAG TGGAAGACGT AAAGCTAATC CACAGGATAA AGTACATCCC AATAAAGCGG
121	AATTCATCAG AGCCAAGTAT CAGATGTTAG CGTTCGTCCA TCGCTTGCCC TGCCGGGATG
181	ACGATAGTGT GACTGCCAAA GATCTTAGCA AGCAACTCCA TTCGAGCGTG AGAACAGGGA
241	ATCTTGAAAC CTGTTTGAGA CTGTTATCTT TAGGAGCACA AGCCAACTTC TTTCATCCTG
301	AAAAAGGAAA CACCCCACTC CATGTTGCCT CCAAAGCAGG GCAGATTTTA CAGGCTGAAT
361	TATTGGCAGT ATATGGAGCA GACCCAGGCA CACAGGATTC TAGTGGGAAA ACTCCCGTTG
421	ATTATGCAAG GCAAGGAGGG CACCATGAGC TGGCAGAGCG CCTCGTGGAA ATACAGTATG
481	AGCTAACGGA CAGACTAGCC TTCTATCTCT GTGGCAGGAA ACCAGATCAC AAAAATGGAC
541	AGCACTTTAT AATACCTCAA ATGGCAGACA GCAGCCTGGA TTTGTCTGAA TTGGCAAAAG
601	CTGCTAAGAA GAAACTTCAA TCTCTAAGTA ATCATTTGTT TGAAGAACTT GCCATGGATG
661	TGTACGATGA AGTTGACAGG CGAGAGACGG ATGCAGTCTG GCTTGCCACG CAAAACCACA
721	GCGCCCTGGT AACCGAGACA ACGGTCGTCC CCTTTCTTCC GGTCAATCCT GAGTACTCAT
781	CAACACGAAA TCAGGGCAGA CAGAAGTTAG CTCGGTTCAA CGCCCATGAG TTTGCCACGC
841	TGGTCATTGA CATTCTCAGT GACGCCAAGA GGAGACAGCA GGGCAGTTCT CTCTCGGGTT
901	CAAAAGACAA TGTGGAGCTC ATACTGAAAA CCATCAATAA CCAGCACAGC GTTGAGAGTC
961	AAGACAACGA TCAGCCCGAC TATGACAGCG TGGCATCAGA CGAAGACACA GATTTGGAAA
1021	CCACTGCAAG CAAAACAAAC CGGCAGAAGA GCCTAGATTC AGATTTATCA GATGGACCAG
1081	TCACTGTACA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG GCCAAGATAC
1141	AGCAGCTAAT GAAGGCATCC AGGCTGGAGA AGCAGAACAG CACACCTGAG AGTGACTACG
1201	ACAACACTCC CAACGACATG GAGCCAGATG GCATGGGGTC AAGCCGAAAG GGACGGCAAA
1261	GAAGTATGGT GTGGCCAGGG GATGGCTTGG TACCAGACAC AGCAGAACCC CATGTGGCCC
1321	CAAGCCCCAC TCTCCCTAGC ACCGAAGATG TCATCAGGAA GACTGAACAG ATCACCAAAA
1381	ACATACAGGA GCTCTTAAGA GCAGCCCAAG AAAATAAACA TGACAGTTAT ATTCCCTGCT
1441	CAGAGAGGAT ACACGTAGCT GTTACAGAAA TGGCAGCATT ATTCCCCAAA AAACCCAAGT
1501	CTGATATGGT GAGGACTTCC CTTCGTTTAC TGACGTCCAG TGCCTACCGA CTGCAGTCAG
1561	AGTGCAAGAA GACCCTCCCA GGGGACCCCG GCTCACCCAC AGACGTTCAG CTGGTCACGC
1621	AGCAGGTCAT CCAGTGTGCG TACGACATCG CCAAGGCTGC CAAGCAGCTG GTTACCATCA
1681	CCACCAAAGA GAACAACAAC TGCCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc
1741	attgcttatc aatttgttgc aacgaac

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