Transcript: Human NM_057170.5

Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
GIT2 (9815)
Length:
5214
CDS:
145..2040

Additional Resources:

NCBI RefSeq record:
NM_057170.5
NBCI Gene record:
GIT2 (9815)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_057170.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000364545 ATAACGGTGCTAACTCTATAT pLKO_005 350 CDS 100% 13.200 18.480 N GIT2 n/a
2 TRCN0000364618 ACAACGATCAGCCCGACTATG pLKO_005 1301 CDS 100% 10.800 15.120 N GIT2 n/a
3 TRCN0000364544 GAGTACTCATCAACACGAAAT pLKO_005 1108 CDS 100% 10.800 15.120 N GIT2 n/a
4 TRCN0000008406 CCTTGTATAATAACGGTGCTA pLKO.1 341 CDS 100% 2.640 3.696 N GIT2 n/a
5 TRCN0000364617 GCCTATGGCTGAAACTATAAA pLKO_005 2366 3UTR 100% 15.000 10.500 N GIT2 n/a
6 TRCN0000369246 AGGCTTTATAAAGTCCAATTT pLKO_005 2072 3UTR 100% 13.200 9.240 N GIT2 n/a
7 TRCN0000008408 GCTGAATTATTGGCAGTATAT pLKO.1 691 CDS 100% 13.200 9.240 N GIT2 n/a
8 TRCN0000364546 TTGCTGGACCCTGCGTCTATT pLKO_005 382 CDS 100% 13.200 9.240 N GIT2 n/a
9 TRCN0000369247 CCACGCTGGTCATTGACATTC pLKO_005 1172 CDS 100% 10.800 7.560 N GIT2 n/a
10 TRCN0000008405 CCAAGCATGTATGTAGAGAAA pLKO.1 2523 3UTR 100% 4.950 3.465 N GIT2 n/a
11 TRCN0000369173 AGAGCCTAGATTCAGATTTAT pLKO_005 1385 CDS 100% 15.000 9.000 N GIT2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_057170.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11418 pDONR223 100% 86.3% 86.3% None 1_258del n/a
2 ccsbBroad304_11418 pLX_304 0% 86.3% 86.3% V5 1_258del n/a
3 TRCN0000465676 TGCATATTGTGGGCGCGACGGAGA pLX_317 22% 86.3% 86.3% V5 1_258del n/a
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