Transcript: Human XM_006719709.4

PREDICTED: Homo sapiens GIT ArfGAP 2 (GIT2), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GIT2 (9815)
Length:
3007
CDS:
71..2200

Additional Resources:

NCBI RefSeq record:
XM_006719709.4
NBCI Gene record:
GIT2 (9815)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006719709.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000364545 ATAACGGTGCTAACTCTATAT pLKO_005 276 CDS 100% 13.200 18.480 N GIT2 n/a
2 TRCN0000364618 ACAACGATCAGCCCGACTATG pLKO_005 1227 CDS 100% 10.800 15.120 N GIT2 n/a
3 TRCN0000364544 GAGTACTCATCAACACGAAAT pLKO_005 1034 CDS 100% 10.800 15.120 N GIT2 n/a
4 TRCN0000008406 CCTTGTATAATAACGGTGCTA pLKO.1 267 CDS 100% 2.640 3.696 N GIT2 n/a
5 TRCN0000364617 GCCTATGGCTGAAACTATAAA pLKO_005 2526 3UTR 100% 15.000 10.500 N GIT2 n/a
6 TRCN0000369246 AGGCTTTATAAAGTCCAATTT pLKO_005 2232 3UTR 100% 13.200 9.240 N GIT2 n/a
7 TRCN0000008408 GCTGAATTATTGGCAGTATAT pLKO.1 617 CDS 100% 13.200 9.240 N GIT2 n/a
8 TRCN0000364546 TTGCTGGACCCTGCGTCTATT pLKO_005 308 CDS 100% 13.200 9.240 N GIT2 n/a
9 TRCN0000369247 CCACGCTGGTCATTGACATTC pLKO_005 1098 CDS 100% 10.800 7.560 N GIT2 n/a
10 TRCN0000008405 CCAAGCATGTATGTAGAGAAA pLKO.1 2683 3UTR 100% 4.950 3.465 N GIT2 n/a
11 TRCN0000008409 CCAATGTATATCAGGTGCAAA pLKO.1 1362 CDS 100% 4.950 3.465 N GIT2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006719709.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11418 pDONR223 100% 75.3% 73.6% None (many diffs) n/a
2 ccsbBroad304_11418 pLX_304 0% 75.3% 73.6% V5 (many diffs) n/a
3 TRCN0000465676 TGCATATTGTGGGCGCGACGGAGA pLX_317 22% 75.3% 73.6% V5 (many diffs) n/a
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