Construct: ORF ccsbBroadEn_11674

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005142.1_s300c1, BRDN0000391746
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KIF1B (23095)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23095 KIF1B kinesin family member 1B NM_015074.3 32.6% 32.7% 1_3573del;5163C>A
2 human 23095 KIF1B kinesin family member 1B NM_001365951.2 31.8% 31.8% 1_3711del;5301C>A
3 human 23095 KIF1B kinesin family member 1B NM_001365952.1 31.8% 31.8% 1_3711del;5301C>A
4 mouse 16561 Kif1b kinesin family member 1B XM_006538595.3 30.4% 32.7% (many diffs)
5 mouse 16561 Kif1b kinesin family member 1B XM_006538594.3 29.7% 31.9% (many diffs)
6 mouse 16561 Kif1b kinesin family member 1B NM_207682.2 29.1% 31.2% (many diffs)
7 mouse 16561 Kif1b kinesin family member 1B XM_006538593.3 29% 31.1% (many diffs)
8 mouse 16561 Kif1b kinesin family member 1B XM_006538592.3 28.7% 30.9% (many diffs)
9 mouse 16561 Kif1b kinesin family member 1B XM_006538591.3 28.5% 30.7% (many diffs)
10 mouse 16561 Kif1b kinesin family member 1B XM_006538590.3 28.4% 30.6% (many diffs)
11 mouse 16561 Kif1b kinesin family member 1B NM_001290995.1 28.3% 30.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1803
ORF length:
1737
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG CAAAACCAGC CTTGGCCAGA GCATGAGCAA GTATGACCTC CTGGTTTGGT
121TTGAGATCAG TGAACTGGAG CCTACAGGAG AGTATATCCC AGCTGTGGTT GACCACACAG
181CAGGCTTGCC TTGCCAGGGG ACATTTTTGC TTCATCAGGG CATCCAGCGA AGGATCACAG
241TGACCATTAT CCATGAGAAG GGGAGCGAGC TCCATTGGAA AGATGTTCGT GAACTGGTGG
301TAGGTCGTAT TCGGAATAAG CCTGAGGTGG ATGAAGCTGC AGTTGATGCC ATCCTCTCCC
361TAAATATTAT TTCTGCCAAG TACCTGAAGT CTTCCCACAA CTCTAGCAGG ACCTTCTACC
421GCTTTGAGGC TGTGTGGGAT AGCTCTCTGC ATAACTCCCT TCTTCTGAAC CGAGTGACAC
481CCTATGGAGA AAAGATCTAC ATGACCTTGT CGGCCTACCT AGAGCTGGAT CATTGCATCC
541AGCCGGCTGT CATCACCAAG GATGTGTGCA TGGTCTTCTA CTCCCGAGAT GCCAAGATCT
601CACCACCACG CTCTCTGCGT AGCCTCTTTG GCAGCGGCTA CTCAAAGTCA CCAGATTCGA
661ATCGAGTCAC TGGCATTTAC GAACTCAGCT TATGCAAAAT GTCAGACACA GGTAGTCCAG
721GTATGCAGAG AAGGAGAAGA AAAATCTTAG ATACGTCAGT GGCATATGTG CGGGGAGAAG
781AGAACTTAGC AGGCTGGCGG CCCCGTGGAG ACAGCCTCAT CCTTGAGCAC CAGTGGGAGC
841TGGAGAAGCT GGAGCTCCTA CATGAGGTGG AAAAAACCCG CCACTTTTTG CTGCTGCGTG
901AGAGACTTGG TGACAGCATC CCCAAATCCC TGAGCGACTC GTTATCCCCC AGCCTCAGCA
961GTGGGACCCT CAGCACCTCC ACCAGTATCT CCTCTCAGAT CTCAACCACT ACCTTTGAAA
1021GCGCCATCAC ACCTAGCGAG AGCAGTGGCT ATGATTCAGG AGACATCGAA AGCCTGGTGG
1081ACCGAGAGAA AGAGCTGGCT ACCAAGTGCC TGCAACTTCT CACCCACACT TTCAACAGAG
1141AATTCAGCCA GGTGCACGGC AGCGTCAGTG ACTGTAAGTT GTCTGATATC TCTCCAATTG
1201GACGGGATCC CTCTGAGTCC AGTTTCAGCA GTGCCACCCT CACTCCCTCC TCCACCTGTC
1261CCTCTCTGGT AGACTCTAGG AGCAACTCTC TGGATCAGAA GACCCCAGAA GCCAATTCCC
1321GGGCCTCTAG TCCCTGCCCA GAATTTGAAC AGTTTCAGAT TGTCCCAGCT GTGGAAACAC
1381CATATTTGGC CCGAGCAGGA AAAAACGAAT TTCTCAATCT TGTTCCAGAT ATTGAAGAAA
1441TTAGACCAAG CTCAGTGGTC TCTAAGAAAG GATACCTTCA TTTCAAGGAG CCTCTTTACA
1501GTAACTGGGC TAAACATTTT GTTGTCGTCC GTCGGCCTTA TGTCTTCATC TATAACAGTG
1561ACAAAGACCC TGTGGAGCGT GGAATCATTA ACCTGTCCAC AGCACAGGTG GAGTACAGTG
1621AGGACCAGCA GGCCATGGTG AAGACACCAA ACACATTTGC TGTCTGCACA AAGCACCGTG
1681GGGTCCTTTT GCAGGCCCTC AATGACAAAG ACATGAACGA CTGGTTGTAT GCCTTCAACC
1741CACTTCTAGC TGGCACAATA CGGTCAAAGC TTTCCCGCAG ATGCCCGAGC CAGTCGAAAT
1801ACTACCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt
1861gcaacgaac

Download FASTA (ORF) (Full)