Construct: ORF ccsbBroadEn_11867
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000780.1_s300c1, BRDN0000384906
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCLO (27445)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27445 | PCLO | piccolo presynaptic cytomat... | XM_017012007.1 | 1.6% | 1.3% | 1_6561del;6660_6667delTGTAAGAC;6693_7683del |
| 2 | human | 27445 | PCLO | piccolo presynaptic cytomat... | XM_017012006.2 | 1.4% | 1.1% | 1_6561del;6660_6667delTGTAAGAC;6693_8514del |
| 3 | human | 27445 | PCLO | piccolo presynaptic cytomat... | NM_014510.3 | .8% | .6% | 1_13656del;13755_13762delTGTAAGAC;13788_14805del |
| 4 | human | 27445 | PCLO | piccolo presynaptic cytomat... | NM_033026.6 | .7% | .6% | 1_13656del;13755_13762delTGTAAGAC;13788_15426del |
| 5 | human | 27445 | PCLO | piccolo presynaptic cytomat... | XR_001744643.2 | .5% | 1_15225del;15324_15331delTGTAAGAC;15357_22942del | |
| 6 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | XM_006503586.3 | .8% | .8% | (many diffs) |
| 7 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | NM_001110796.2 | .7% | .6% | (many diffs) |
| 8 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | XM_006503584.3 | .7% | .6% | (many diffs) |
| 9 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | NM_011995.4 | .7% | .6% | (many diffs) |
| 10 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | XM_006503583.3 | .7% | .6% | (many diffs) |
| 11 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | XM_006503582.3 | .7% | .6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 189
- ORF length:
- 123
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCA AGTATTGGAA TGGAATGGAA TTCCCTTGAC TTCTAAAACA TATGAAGAAG 121 TTCAGAGTAT CATTAGTCAG CAAAGTGGGG AAGCAGAAAT ATGTGGACCT CAATATGCTA 181 TCAGATTCTG CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa 241 tttgttgcaa cgaac