Construct: ORF ccsbBroadEn_11986

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001432.1_s300c1, BRDN0000387087
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
POLK (51426)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51426 POLK DNA polymerase kappa NM_016218.3 53.8% 52.1% (many diffs)
2 human 51426 POLK DNA polymerase kappa XM_017009559.2 53.8% 52.1% (many diffs)
3 human 51426 POLK DNA polymerase kappa XM_017009560.2 53.8% 52.1% (many diffs)
4 human 51426 POLK DNA polymerase kappa XM_005248534.5 53% 51.2% (many diffs)
5 human 51426 POLK DNA polymerase kappa XM_011543463.3 53% 51.2% (many diffs)
6 human 51426 POLK DNA polymerase kappa XM_011543464.3 53% 51.2% (many diffs)
7 human 51426 POLK DNA polymerase kappa NM_001345921.1 46.2% 44.5% (many diffs)
8 human 51426 POLK DNA polymerase kappa XM_017009561.2 45.5% 43.8% (many diffs)
9 human 51426 POLK DNA polymerase kappa NM_001345922.1 43.5% 41.8% (many diffs)
10 human 51426 POLK DNA polymerase kappa XM_017009563.2 43.5% 41.8% (many diffs)
11 human 51426 POLK DNA polymerase kappa XM_011543467.3 42.8% 41.1% (many diffs)
12 human 51426 POLK DNA polymerase kappa XR_002956163.1 36.1% (many diffs)
13 human 51426 POLK DNA polymerase kappa XR_001742108.2 31.5% (many diffs)
14 human 51426 POLK DNA polymerase kappa XR_948273.3 30% (many diffs)
15 human 51426 POLK DNA polymerase kappa XR_001742105.2 28.5% (many diffs)
16 human 51426 POLK DNA polymerase kappa XR_241784.3 28.4% (many diffs)
17 human 51426 POLK DNA polymerase kappa XR_001742107.2 27.7% (many diffs)
18 human 51426 POLK DNA polymerase kappa NR_144315.1 13.4% (many diffs)
19 mouse 27015 Polk polymerase (DNA directed), ... NM_001347604.1 73% 74% (many diffs)
20 mouse 27015 Polk polymerase (DNA directed), ... NM_001347606.1 60.8% 62.4% (many diffs)
21 mouse 27015 Polk polymerase (DNA directed), ... NM_012048.3 47.1% 47.6% (many diffs)
22 mouse 27015 Polk polymerase (DNA directed), ... NM_001347602.1 41.1% 40.3% (many diffs)
23 mouse 27015 Polk polymerase (DNA directed), ... XM_006517680.3 40.4% 40.4% (many diffs)
24 mouse 27015 Polk polymerase (DNA directed), ... NM_001347603.1 39.2% 40.1% (many diffs)
25 mouse 27015 Polk polymerase (DNA directed), ... NR_144622.1 29.7% (many diffs)
26 mouse 27015 Polk polymerase (DNA directed), ... XR_001780788.1 27.8% (many diffs)
27 mouse 27015 Polk polymerase (DNA directed), ... NR_144623.1 24.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1482
ORF length:
1416
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTAGCATGGA TAGCACAAAG GAGAAGTGTG ACAGTTACAA AGATGATCTT CTGCTTAGGA
121TGGGACTTAA TGATAATAAA GCAGGAATGG AAGGATTAGA TAAAGAGAAA ATTAACAAAA
181TTATAATGGA AGCCACGAAG GGGTCCAGAT TTTATGGAAA TGAGCTCAAG AAAGAAAAGC
241AAGTCAACCA ACGAATTGAA AATATGATGC AACAAAAAGC TCAAATCACC AGCCAACAGC
301TAAGAAAAGC ACAATTACAG GTTGACAGAT TTGCAATGGA ATTAGAACAA AGCCGAAATT
361TGAGCAATAC CATAGTGCAC ATTGACATGG ATGCTTTCTA TGCAGCTGTA GAAATGAGGG
421ACAATCCAGA ATTGAAGGAT AAACCCATTG CTGTAGGATC AATGAGTATG CTGTCTACTT
481CAAATTACCA TGCAAGGAGA TTTGGTGTTC GTGCAGCCAT GCCAGGATTT ATTGCTAAGA
541GGCTGTGCCC ACAACTTATA ATAGTGCCCC CCAACTTTGA CAAATACCGA GCTGTGAGTA
601AAGAGGTTAA GGAAATACTT GCTGATTATG ATCCCAATTT TATGGCCATG AGTCTTGATG
661AAGCCTACTT GAATATAACA AAGCACTTAG AAGAAAGACA AAATTGGCCT GAGGATAAAA
721GAAGGTATTT CATCAAAATG GGAAGCTCTG TAGAAAATGA TAATCCAGGA AAGGAAGTTA
781ATAAACTGAG TGAGCATGAA CGATCCATCT CTCCACTACT TTTTGAAGAG AGTCCTTCTG
841ATGTGCAGCC CCCAGGAGAT CCTTTCCAAG TGAACTTTGA AGAACAAAAC AATCCTCAAA
901TACTCCAAAA CTCAGTTGTT TTTGGAACAT CAGCCCAGGA AGTGGTAAAG GAAATTCGTT
961TCAGAATTGA GCAGAAAACA ACACTGACAG CCAGTGCAGG CATTGCCCCA AATACAATGT
1021TAGCAAAAGT ATGCAGTGAT AAGAATAAAC CAAATGGACA ATACCAAATT CTTCCCAATA
1081GACAAGCTGT GATGGACTTC ATCAAGGATT TACCCATTAG AAAGGTTTCT GGAATAGGAA
1141AAGTTACAGA GAAAATGTTA AAGGCCCTTG GAATTATTAC ATGTACAGAA CTTTACCAAC
1201AGAGGGCATT GCTTTCTCTC CTTTTCTCTG AAACATCTTG GCATTATTTC CTTCATATCT
1261CCTTGGGTCT AGGTTCAACA CACCTGACGA GGGATGGAGA GAGGAAAAGT ATGAGCGTTG
1321AGAGGACATT CAGTGAGATA AATAAAGCGG AAGAGCAATA CAGCCTATGT CAAGAACTTT
1381GCAGTGAGCT TGCTCAGGAT CTACAGAAAG AAAGACTTAA GGTGCTTTAT TTTGATATGG
1441TGTCCTTAGT TTTTAAGTTT TTTAATAGTA AAATGCTACC TTACCCAACT TTCTTGTACA
1501AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac

Download FASTA (ORF) (Full)

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