Transcript: Mouse XM_006517680.3

PREDICTED: Mus musculus polymerase (DNA directed), kappa (Polk), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Polk (27015)
Length:
4225
CDS:
381..2744

Additional Resources:

NCBI RefSeq record:
XM_006517680.3
NBCI Gene record:
Polk (27015)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006517680.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000329098 GAATTTCATTCCCAGTATATA pLKO_005 2933 3UTR 100% 15.000 21.000 N Polk n/a
2 TRCN0000120329 GCTCAATTACAGGTTGACAAA pLKO.1 426 CDS 100% 4.950 6.930 N Polk n/a
3 TRCN0000329036 GCTCAATTACAGGTTGACAAA pLKO_005 426 CDS 100% 4.950 6.930 N Polk n/a
4 TRCN0000120331 CCGGAATTTGAACAATACCAT pLKO.1 470 CDS 100% 3.000 4.200 N Polk n/a
5 TRCN0000329037 CCGGAATTTGAACAATACCAT pLKO_005 470 CDS 100% 3.000 4.200 N Polk n/a
6 TRCN0000120328 GCCCTTAGAAATGTCTCATAA pLKO.1 1853 CDS 100% 13.200 10.560 N Polk n/a
7 TRCN0000329097 GCCCTTAGAAATGTCTCATAA pLKO_005 1853 CDS 100% 13.200 10.560 N Polk n/a
8 TRCN0000329099 ATGTTGGCTACTTCGAATTAC pLKO_005 585 CDS 100% 13.200 9.240 N Polk n/a
9 TRCN0000120330 GCAAAGAGGAATGTCCTGATA pLKO.1 2341 CDS 100% 4.950 3.465 N Polk n/a
10 TRCN0000120327 GCACAGTCTTAACCTGACTTT pLKO.1 3939 3UTR 100% 4.950 3.465 N Polk n/a
11 TRCN0000054408 ACGCCTTTAATCCCAGCACTT pLKO.1 3693 3UTR 100% 4.050 2.025 Y Mtif2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006517680.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11986 pDONR223 100% 40.4% 40.4% None (many diffs) n/a
2 ccsbBroad304_11986 pLX_304 0% 40.4% 40.4% V5 (many diffs) n/a
3 TRCN0000471624 TTAGCCATCACTAACGCGATGTAG pLX_317 35.7% 40.4% 40.4% V5 (many diffs) n/a
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