Construct: ORF ccsbBroadEn_12156

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000095.1_s300c1, BRDN0000395436
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATG2B (55102)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55102 ATG2B autophagy related 2B NM_018036.7 34.7% 34.6% 1_4068del;4700T>C
2 mouse 76559 Atg2b autophagy related 2B XM_006516361.2 60.6% 63.3% (many diffs)
3 mouse 76559 Atg2b autophagy related 2B XM_006516360.1 29.7% 31% (many diffs)
4 mouse 76559 Atg2b autophagy related 2B NM_029654.4 29.6% 30.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2232
ORF length:
2166
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA TCTCATTCAG TACATTGCAA GCTATGGTGA CTTGCAGACA CCTAACAAGG
121CAGATATGAA GCCTGGAGCC TTTCAAAGAA GGTCTAAGGT AGATTCCAGT GGTCGATCAT
181CCTCACGTGG TCCAGTACTT CCTGAAGCAG ATCAACAAAT GTTACGAGAT CTGATGAGTG
241ATGCTATGGA GGAGATCGAC ATGCAACAAG GCACCTCGTC AGTAAAACCA CAGGCTAATG
301GTGTTTTGGA TGAAAAATCT CAAATTCAGG AGCCATGTTG TTCAGACCTC TTCCTGTTTC
361CTGACGAGAG TGGGAATGTA TCCCAGGAGT CCGGCCCCAC CTATGCCTCA TTCTCTCACC
421ATTTCATCAG TGATGCAATG ACAGGTGTGC CCACTGAGAA TGATGACTTT TGCATTCTTT
481TTGCACCAAA AGCAGCCATG CAGGAGAAGG AAGAAGAACC AGTTATAAAA ATCATGGTTG
541ATGATGCAAT TGTGATAAGA GACAATTATT TCAGTCTGCC CGTTAATAAG ACCGATACGA
601GCAAAGCCCC CTTACACTTT CCCATTCCTG TGATTCGCTA TGTGGTGAAG GAGGTCTCTC
661TTGTCTGGCA TCTTTATGGA GGAAAGGATT TTGGAACAGT CCCTCCCACT TCTCCGGCTA
721AAAGTTATAT TAGTCCCCAC AGTTCGCCTT CTCACACACC CACGAGACAT GGACGTAATA
781CAGTATGTGG GGGAAAAGGA AGGAACCATG ACTTTTTAAT GGAAATACAG CTAAGCAAGG
841TGAAGTTTCA GCATGAAGTC TACCCGCCAT GCAAACCTGA TTGTGATTCC AGCCTCTCAG
901AACACCCAGT CTCCCGGCAG GTGTTCATTG TTCAGGATCT TGAGATTCGA GATCGTTTGG
961CAACATCACA AATGAATAAA TTTTTATACC TGTATTGCAG TAAAGAAATG CCTCGAAAAG
1021CTCACTCCAA CATGTTGACA GTGAAAGCCT TACACGTGTG TCCAGAATCT GGCAGGTCCC
1081CACAGGAGTG CTGCTTGAGA GTGTCGCTGA TGCCGCTCCG CCTCAATATT GACCAGGATG
1141CTTTGTTCTT CCTGAAGGAT TTCTTCACAA GTCTTTCTGC AGAAGTAGAG CTTCAAATGA
1201CTCCAGATCC AGAAGTTAAA AAGTCTCCTG GAGCTGATGT CACCTGCAGT TTGCCAAGGC
1261ATTTGAGTAC CTCAAAGGAG CCAAATCTGG TTATTTCTTT CTCTGGGCCA AAACAGCCTT
1321CCCAAAATGA TAGTGCCAAT TCAGTGGAAG TGGTTAATGG CATGGAAGAG AAGAACTTCT
1381CTGCTGAAGA AGCATCTTTT AGGGATCAGC CTGTGTTTTT TAGAGAATTT AGATTCACGT
1441CAGAAGTTCC CATTCGACTT GATTATCATG GCAAACATGT ATCAATGGAT CAGGGTACGC
1501TAGCTGGGAT TTTGATTGGT CTGGCTCAGT TAAACTGCTC TGAACTAAAG CTCAAGAGGC
1561TTTCCTATCG ACATGGTTTA CTAGGCGTTG ACAAATTATT CTCATATGCA ATCACTGAGT
1621GGCTTAATGA CATTAAGAAG AACCAGCTAC CAGGAATCCT GGGAGGTGTT GGACCTATGC
1681ATTCACTAGT ACAATTAGTA CAAGGCCTAA AGGACTTGGT CTGGCTCCCA ATAGAGCAGT
1741ACCGGAAGGA TGGCCGCATT GTCAGAGGGT TTCAGAGAGG CGCTGCTTCC TTTGGTACCT
1801CGACAGCGAT GGCTGCTCTA GAACTCACAA ACAGAATGGT TCAAACCATA CAGGCAGCTG
1861CAGAGACTGC TTATGATATG GTGTCTCCTG GTACCCTTTC TATCGAGCCC AAGAAGACCA
1921AAAGGTTTCC TCATCACCGG TTAGCCCACC AGCCAGTAGA CCTGAGGGAA GGTGTGGCCA
1981AGGCCTACAG TGTTGTGAAA GAGGGAATCA CAGACACGGC TCAGACCATT TATGAAACTG
2041CGGCTCGAGA ACACGAGAGC AGAGGGGTGA CTGGTGCCGT GGGCGAGGTT CTGCGCCAGA
2101TTCCTCCGGC AGTGGTGAAA CCTCTGATTG TTGCCACAGA AGCAACGTCA AACGTGCTGG
2161GTGGCATGAG AAACCAAATT AGGCCAGATG TCCGGCAAGA CGAGTCACAG AAATGGCGCC
2221ACGGGGATGA CTGCCCAACT TTCTTGTACA AAGTtggcat tataagaaag cattgcttat
2281caatttgttg caacgaac

Download FASTA (ORF) (Full)