Construct: ORF ccsbBroadEn_12195

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013675.1_s300c1, BRDN0000391900
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VPS53 (55275)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55275 VPS53 VPS53 subunit of GARP complex XM_017024818.1 19.5% 19.3% (many diffs)
2 human 55275 VPS53 VPS53 subunit of GARP complex XM_017024817.2 17.7% 17.5% (many diffs)
3 human 55275 VPS53 VPS53 subunit of GARP complex NM_001128159.3 16.6% 16.4% (many diffs)
4 human 55275 VPS53 VPS53 subunit of GARP complex XR_001752553.2 8.3% (many diffs)
5 human 55275 VPS53 VPS53 subunit of GARP complex XR_934061.3 3.9% (many diffs)
6 mouse 68299 Vps53 VPS53 GARP complex subunit XM_006534053.3 17.2% 18.3% (many diffs)
7 mouse 68299 Vps53 VPS53 GARP complex subunit XM_006534052.3 15.1% 16.1% (many diffs)
8 mouse 68299 Vps53 VPS53 GARP complex subunit NM_026664.3 14.6% 15.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
486
ORF length:
420
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG TTTGCTGCTG CTGGACACCC ACTCGCTGAA GATGGTCCTG CTCGATCTCC
121CCTCCATCAG CTCGCAGGTG GTGAGGAAGG CACCCGCCAG CTACACCAAG ATCGTTGTCA
181AAGGCATGAC CCGGGCTGAG ATGATCCTCA AGGTAGTGAT GGCCCCTCAT GAACCGTTGG
241TGGTGTTTGT TGACAACTAC ATCAAACTTC TCACAGACTG CAACACAGAA ACCTTTCAGA
301AGATACTGGA CATGAAGGGG CTGAAGAGGA GTGAGCAGAG CAGCATGCTG GAACTCCTGC
361GCCAGCGGCT CCCCGCACCG CCCTCGGGGG CAGAAAGCTC CGGCTCACTG TCCCTGACGG
421CGCCGACACC AGAGCAAGAG TCGTCACGCA TCCGCAAGCT CGAGAAACTC ATTAAAAAGA
481GACTGTACCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt
541gttgcaacga ac

Download FASTA (ORF) (Full)