Construct: ORF ccsbBroadEn_12196

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF008977.1_s300c1, BRDN0000395797
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: FGGY (55277)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_001278224.2	100%	100%
2	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_024448227.1	73.4%	73.4%	1_273del
3	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_024448229.1	73.4%	73.4%	1_273del
4	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001677.1	68.6%	68.6%	1_273del;597_668del
5	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001678.1	68.6%	68.6%	1_273del;597_668del
6	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_001350797.2	60.4%	60.4%	1_495del
7	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_001350796.2	57.4%	57.4%	1_561del
8	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001673.2	57.4%	57.4%	1_561del
9	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_024448220.1	57.4%	57.4%	1_561del
10	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001668.1	57.1%	57.1%	1_495del;819_890del
11	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001669.2	57.1%	57.1%	1_495del;819_890del
12	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001670.2	57.1%	57.1%	1_495del;819_890del
13	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001671.1	57.1%	57.1%	1_495del;819_890del
14	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_001244714.2	54.4%	54.4%	1_633del
15	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001656.1	54.4%	54.4%	1_561del;885_956del
16	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001657.2	54.4%	54.4%	1_561del;885_956del
17	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001659.2	54.4%	54.4%	1_561del;885_956del
18	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001660.2	54.4%	54.4%	1_561del;885_956del
19	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001661.2	54.4%	54.4%	1_561del;885_956del
20	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001662.2	54.4%	54.4%	1_561del;885_956del
21	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001663.2	54.4%	54.4%	1_561del;885_956del
22	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001664.2	54.4%	54.4%	1_561del;885_956del
23	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_001350795.2	51.7%	51.7%	1_633del;957_1028del
24	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_011541736.3	51.7%	51.7%	1_633del;957_1028del
25	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001655.1	51.7%	51.7%	1_633del;957_1028del
26	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_001350792.2	50.9%	50.9%	1_729del
27	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_001350793.2	50.9%	50.9%	1_729del
28	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_024448185.1	48.5%	48.5%	1_729del;1053_1124del
29	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_024448196.1	48.5%	48.5%	1_729del;1053_1124del
30	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_001350791.2	45.7%	45.7%	1_897del
31	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_018291.4	45.7%	45.7%	1_897del
32	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001645.1	45.7%	45.7%	1_897del
33	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001646.1	45.7%	45.7%	1_897del
34	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_001113411.2	43.8%	43.8%	1_897del;1221_1292del
35	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_001350790.2	43.8%	43.8%	1_897del;1221_1292del
36	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_011541730.1	43.8%	43.8%	1_897del;1221_1292del
37	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_011541731.1	43.8%	43.8%	1_897del;1221_1292del
38	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001643.2	43.8%	43.8%	1_897del;1221_1292del
39	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_024448176.1	43.8%	43.8%	1_897del;1221_1292del
40	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_001350799.2	43.7%	40.2%	1_561del;1080_1081ins95;1137_1138ins85
41	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_001350798.2	41.4%	38.1%	(many diffs)
42	human	55277	FGGY	FGGY carbohydrate kinase do...	NM_001350794.2	34.8%	32.1%	1_897del;1416_1417ins95;1473_1474ins85
43	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001652.1	34.8%	32.1%	1_897del;1416_1417ins95;1473_1474ins85
44	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_024448207.1	34.8%	32.1%	1_897del;1416_1417ins95;1473_1474ins85
45	human	55277	FGGY	FGGY carbohydrate kinase do...	XM_017001649.1	33.3%	30.7%	(many diffs)
46	human	55277	FGGY	FGGY carbohydrate kinase do...	XR_001737286.1	31.7%		1_1010del;1185_1186ins148;1619_1767del
47	human	55277	FGGY	FGGY carbohydrate kinase do...	XR_001737285.1	31.3%		1_1033del;1208_1209ins148;1642_1790del
48	human	55277	FGGY	FGGY carbohydrate kinase do...	XR_001737284.1	30.6%		1_1078del;1253_1254ins148;1687_1835del
49	human	55277	FGGY	FGGY carbohydrate kinase do...	NR_103473.2	28.9%		(many diffs)
50	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_006503476.1	89.3%	92.4%	(many diffs)
51	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_017320432.1	65.7%	67.9%	(many diffs)
52	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_006503474.1	51.4%	53%	(many diffs)
53	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_011240637.2	42.1%	38.4%	(many diffs)
54	mouse	75578	Fggy	FGGY carbohydrate kinase do...	NM_001113412.1	40.9%	42.2%	(many diffs)
55	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_006503461.3	40.9%	42.2%	(many diffs)
56	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_011240633.2	40.3%	41.6%	(many diffs)
57	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_011240636.1	35.5%	35.8%	(many diffs)
58	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_011240635.1	35.2%	34.8%	(many diffs)
59	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_011240639.2	34.4%	34.8%	(many diffs)
60	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_017320430.1	31.7%	25%	(many diffs)
61	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_017320429.1	31.2%	24.7%	(many diffs)
62	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_006503468.3	28.7%	23.5%	(many diffs)
63	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_006503472.3	28%	23.9%	(many diffs)
64	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_011240644.1	28%	23.3%	(many diffs)
65	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_006503470.3	27.7%	23.6%	(many diffs)
66	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XM_011240643.2	27.6%	23.6%	(many diffs)
67	mouse	75578	Fggy	FGGY carbohydrate kinase do...	XR_001784213.1	19.1%		(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

822

ORF length:

756

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61	TTGGCATGGG GATCAGCAAA GACCCGATTT TTGTACCAGG CGTCTGGGGG CCTTATTTCT
121	CAGCCATGGT ACCTGGGTTC TGGCTGAATG AAGGTGGTCA GAGCGTTACT GGAAAATTGA
181	TAGACCACAT GGTACAAGGC CATGCTGCTT TTCCAGAACT ACAAGTAAAG GCCACAGCCA
241	GATGCCAGAG TATATATGCA TATTTGAACA GTCACCTGGA TCTGATTAAG AAGGCTCAGC
301	CTGTGGGTTT CCTTACTGTT GATTTACATG TTTGGCCAGA TTTCCATGGC AACCGGTCTC
361	CCTTAGCAGA TCTGACACTA AAGGGCATGG TCACCGGATT GAAACTGTCT CAGGACCTTG
421	ATGATCTTGC CATTCTCTAC CTGGCCACAG TTCAAGCCAT TGCTTTGGGG ACTCGCTTCA
481	TTATAGAAGC CATGGAGGCA GCAGGGCACT CAATCAGTAC TCTTTTCCTA TGTGGAGGCC
541	TCAGCAAGAA TCCCCTTTTT GTGCAAATGC ATGCGGACAT TACTGGCATG CCTGTGGTCC
601	TGTCGCAAGA GGTGGAGTCC GTTCTTGTGG GTGCTGCTGT TCTGGGTGCC TGTGCCTCAG
661	GGGATTTCGC TTCTGTACAG GAAGCAATGG CAAAAATGAG CAAAGTTGGG AAAGTTGTGT
721	TCCCGAGACT ACAGGATAAA AAATACTATG ATAAGAAATA CCAAGTATTC CTGAAGCTGG
781	TTGAACACCA GAAGGAGTAT TTGGCGATCA TGAATGATGA CTGCCCAACT TTCTTGTACA
841	AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac

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