Construct: ORF ccsbBroadEn_12249
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016476.1_s300c1, BRDN0000384912
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IWS1 (55677)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XM_017004468.2 | 8.4% | 1.9% | 1_736del;893_1836del |
| 2 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XM_011511450.1 | 6.9% | 1.6% | 1_1372del;1529_2229del |
| 3 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738828.1 | 6.6% | 1_1574del;1731_2329del | |
| 4 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XM_017004467.2 | 6.3% | 1.4% | 1_1357del;1514_2454del |
| 5 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | NM_017969.3 | 6.3% | 1.4% | 1_1357del;1514_2457del |
| 6 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XM_006712626.3 | 6.3% | 1.4% | 1_1372del;1529_2469del |
| 7 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XM_005263707.3 | 6.3% | 1.4% | 1_1372del;1529_2472del |
| 8 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738827.1 | 5.5% | 1_1574del;1731_2795del | |
| 9 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738829.2 | 5.4% | 1_1638del;1795_2859del | |
| 10 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_922960.2 | 5.3% | 1_1574del;1731_2927del | |
| 11 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738826.2 | 5.2% | 1_1638del;1795_2991del | |
| 12 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738832.1 | 5.1% | (many diffs) | |
| 13 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738830.1 | 4.9% | (many diffs) | |
| 14 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738831.1 | 4.9% | (many diffs) | |
| 15 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738833.2 | 4.8% | (many diffs) | |
| 16 | human | 55677 | IWS1 | interacts with SUPT6H, CTD ... | XR_001738834.2 | 4.8% | (many diffs) | |
| 17 | mouse | 73473 | Iws1 | IWS1, SUPT6 interacting pro... | XM_006526291.3 | 6.1% | 1.6% | (many diffs) |
| 18 | mouse | 73473 | Iws1 | IWS1, SUPT6 interacting pro... | XM_006526292.3 | 6.1% | 1.6% | (many diffs) |
| 19 | mouse | 73473 | Iws1 | IWS1, SUPT6 interacting pro... | NM_173441.3 | 6.1% | 1.6% | (many diffs) |
| 20 | mouse | 73473 | Iws1 | IWS1, SUPT6 interacting pro... | XR_386017.2 | 1.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 222
- ORF length:
- 156
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAA GAGAAGGATC TGTTTGGGAG TGACAGTGAG TCAGGCAATG AAGAAGAAAA 121 TCTTATTGCA GACATATTTG GAGAATCTGG TGATGAAGAG GAAGAAGAAT TTACAGGTTT 181 TAACCAAGAA GATCTGGAAG AAGAAAAAGG TGAAACACAG GTACCCAACT TTCTTGTACA 241 AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac