Construct: ORF ccsbBroadEn_12483
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016908.1_s300c1, BRDN0000395032
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAREM1 (64762)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64762 | GAREM1 | GRB2 associated regulator o... | NM_001242409.2 | 100% | 100% | |
| 2 | human | 64762 | GAREM1 | GRB2 associated regulator o... | NM_022751.3 | 99.8% | 99.8% | 1733_1734insCAG |
| 3 | human | 64762 | GAREM1 | GRB2 associated regulator o... | XM_024451234.1 | 86% | 86% | 0_1ins366 |
| 4 | human | 64762 | GAREM1 | GRB2 associated regulator o... | XM_024451235.1 | 86% | 86% | 0_1ins366 |
| 5 | human | 64762 | GAREM1 | GRB2 associated regulator o... | XM_024451236.1 | 85.9% | 85.9% | 0_1ins366;1367_1368insCAG |
| 6 | human | 64762 | GAREM1 | GRB2 associated regulator o... | XM_017025919.1 | 71.4% | 67.7% | (many diffs) |
| 7 | mouse | 381126 | Garem1 | GRB2 associated regulator o... | NM_001033445.2 | 87.9% | 91.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2697
- ORF length:
- 2628
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGACCCGGCG CCCTCGCTGG GCTGCAGCCT CAAGGATGTG AAGTGGAGCT 121 CGGTGGCCGT GCCGCTCGAC CTCCTGGTCA GCACTTACCG GCTGCCCCAG ATCGCGCGCC 181 TGGACAACGG AGAGTGCGTA GAAGGGCTGC GGGAAAATGA CTATCTGCTG ATTCATTCCT 241 GCCGCCAGTG GACCACCATC ACTGCCCACA GCTTGGAGGA GGGTCACTAT GTCATTGGGC 301 CAAAGATAGA GATTCCGGTA CATTATGCAG GGCAATTCAA GCTGCTGGAA CAAGACCGAG 361 ATATAAAGGA GCCAGTGCAA TATTTCAACA GTGTGGAGGA GGTGGCTAAG GCATTTCCTG 421 AACGCGTGTA CGTCATGGAG GATATCACAT TCAACGTGAA GGTTGCTTCA GGTGAATGCA 481 ATGAAGACAC TGAAGTTTAC AACATCACCC TGTGTACTGG GGATGAACTC ACTCTAATGG 541 GGCAGGCAGA AATCCTTTAT GCAAAGACAT TCAAGGAAAA GTCACGACTC AACACAATCT 601 TCAAAAAGAT TGGGAAGCTC AATTCCATCA GCAAGCTGGG AAAAGGCAAA ATGCCGTGCC 661 TCATTTGTAT GAATCACCGG ACCAACGAAA GCATTAGCCT TCCATTCCAG TGCAAGGGCA 721 GATTTAGCAC CCGAAGTCCC CTGGAACTTC AGATGCAAGA GGGCGAACAC ACCATCCGCA 781 ACATTGTGGA GAAAACCAGG CTTCCTGTGA ATGTGACTGT GCCAAGCCCT CCACCGAGAA 841 ACCCATACGA CCTCCACTTC ATCCGTGAGG GGCACCGCTA TAAGTTTGTG AACATCCAGA 901 CCAAGACGGT GGTGGTTTGC TGTGTGCTGC GGAACAACAA GATCCTCCCC ATGCACTTTC 961 CTTTGCACTT GACTGTCCCC AAGTTCAGCC TCCCAGAACA CCTGGTGAAG GGAGAGAGCT 1021 GGCCCGAAAC CCTGGTCCAT CACTGGCTAG GTATCTGCCA AGAACAGTTC GACATCGATG 1081 AGTATTCACG GGCTGTCCGT GATGTGAAAA CCGACTGGAA TGAAGAATGC AAGAGCCCCA 1141 AGAAGGGTCG GTGCTCTGGC CACAACCACG TGCCCAATTC GCTCAGCTAC GCCCGCGATG 1201 AGCTCACCCA GTCCTTCCAC CGACTCTCGG TCTGTGTGTA TGGCAACAAT CTCCATGGCA 1261 ACAGTGAGGT GAACCTTCAT GGTTGCAGGG ACCTGGGGGG AGATTGGGCT CCCTTTCCTC 1321 ATGACATCCT GCCCTATCAG GACTCTGGAG ATAGTGGGAG CGACTACCTT TTCCCAGAAG 1381 CTAGTGAAGA ATCAGCAGGC ATCCCGGGAA AGTCAGAACT TCCCTACGAA GAGCTGTGGC 1441 TGGAGGAAGG CAAGCCCAGC CATCAGCCTC TCACTCGCTC TCTGAGCGAG AAGAACAGAT 1501 GTGATCAGTT TAGAGGTTCT GTCCGATCCA AATGTGCGAC TTCTCCTCTT CCCATCCCTG 1561 GGACTCTGGG AGCAGCAGTG AAGTCTTCAG ATACTGCCCT ACCTCCACCT CCAGTGCCTC 1621 CCAAATCTGA AGCCGTCAGA GAAGAATGCC GGCTCCTGAA CGCCCCACCT GTTCCACCCC 1681 GAAGCGCAAA GCCTTTGTCC ACCAGTCCCT CCATCCCTCC TCGCACAGTC AAGCCAGCGC 1741 GGCAACAGAC TCGCTCTCCC AGCCCCACCT TGTCCTACTA TTCTTCAGGG CTACACAACA 1801 TCAGCGTCAC TAAAACTGAC ACAAATCCTT CTGAAAGCAC TCCTGTTTCC TGCTATCCAT 1861 GTAACCGAGT GAAAACTGAT TCTGTGGACC TGAAATCCCC GTTTGGAAGT CCTTCTGCTG 1921 AAGCTGTGTC CTCTCGGCTC TCATGGCCTA ACCATTATTC AGGAGCATCA GAAAGCCAGA 1981 CCAGGAGTGA CTTCCTGCTG GATCCAAGCA GGAGTTATAG TTACCCTAGA CAAAAGACGC 2041 CAGGCACACC AAAGAGAAAC TGCCCAGCAC CTTTTGATTT TGATGGCTGT GAGCTCCTGG 2101 CCAGCCCCAC TAGCCCAGTC ACTGCAGAAT TCAGTAGCAG CGTCTCTGGT TGTCCCAAGT 2161 CAGCCAGCTA CTCTCTGGAG AGCACAGATG TGAAATCTCT TGCAGCTGGT GTGACAAAGC 2221 AGAGTACGTC ATGCCCTGCC TTACCCCCCA GGGCTCCAAA ACTAGTGGAA GAGAAGGTCG 2281 CCTCCGAAAC ATCTCCTTTG CCTCTGAAAA TTGATGGTGC TGAGGAAGAC CCCAAGTCTG 2341 GGTCACCAGA TCTCTCGGAG GACCAGTATT TTGTTAAAAA GGGCATGCAG GACATCTTCT 2401 CTGCCTCCTA CCCTTTCTCA TCTCCGCTCC ATCTCCAGCT GGCCCCCAGA TCCTGTGGCG 2461 ACGGTTCCCC ATGGCAGCCA CCTGCTGACC TATCAGGACT CTCTATAGAG GAAGTGTCCA 2521 AGTCACTACG GTTCATTGGT TTGTCCGAAG ATGTCATATC ATTCTTTGTT ACTGAAAAGA 2581 TTGATGGGAA CCTGCTTGTT CAGCTAACGG AAGAAATCCT CTCAGAGGAT TTCAAATTGA 2641 GCAAATTGCA GGTGAAGAAG ATAATGCAAT TCATTAATGG CTGGAGGCCC AAAATATTGC 2701 CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg 2761 aac