Construct: ORF ccsbBroadEn_12547

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013829.1_s300c1, BRDN0000385059
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NDUFAF5 (79133)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... NM_001352406.2 100% 100%
2 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... NM_001352407.2 100% 100%
3 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... NM_001352403.2 84% 84% 1_90del
4 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... XM_006723624.2 84% 84% 1_90del
5 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... XM_024451999.1 84% 84% 1_90del
6 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... NM_001039375.3 49.8% 49.8% 1_477del
7 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... NM_024120.5 45.7% 45.7% 1_561del
8 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... XR_937140.2 32.1% (many diffs)
9 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... NM_001352408.1 16.6% 15.2% (many diffs)
10 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... XR_430269.3 9.8% (many diffs)
11 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... NR_147980.2 8.7% 1_498del;713_810del;1071_5443del
12 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... XR_001754396.1 8.6% 1_520del;735_832del;1093_5465del
13 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... NR_147978.2 8.6% 1_602del;758_813del;1133_5505del
14 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... NR_029377.2 8.5% 1_602del;817_914del;1175_5547del
15 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... NR_147979.2 8.5% 1_622del;837_934del;1195_5567del
16 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... NR_147983.2 8.4% 1_652del;867_964del;1225_5597del
17 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... NR_147981.2 8.3% 1_736del;951_1048del;1309_5681del
18 human 79133 NDUFAF5 NADH:ubiquinone oxidoreduct... NR_147982.2 8.2% (many diffs)
19 mouse 69487 Ndufaf5 NADH:ubiquinone oxidoreduct... XM_030252053.1 87.7% 90.5% (many diffs)
20 mouse 69487 Ndufaf5 NADH:ubiquinone oxidoreduct... XM_030252052.1 73.7% 76% (many diffs)
21 mouse 69487 Ndufaf5 NADH:ubiquinone oxidoreduct... XM_011239768.2 43.7% 45.1% (many diffs)
22 mouse 69487 Ndufaf5 NADH:ubiquinone oxidoreduct... NM_027093.4 40.1% 41.4% (many diffs)
23 mouse 69487 Ndufaf5 NADH:ubiquinone oxidoreduct... XR_003953716.1 2.8% (many diffs)
24 mouse 69487 Ndufaf5 NADH:ubiquinone oxidoreduct... XR_003953715.1 2.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
540
ORF length:
474
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTT TGGAGGCGAC ACACTCTATG AACTTCGGTG TTCCTTACAG TTAGCGGAAA
121CGGAAAGGGA AGGAGGATTT TCTCCACACA TTTCTCCTTT CACTGCTGTC AATGACCTGG
181GACATCTGCT TGGGAGAGCT GGCTTTAATA CTCTGACTGT GGACACTGAT GAAATTCAAG
241TTAACTATCC TGGAATGTTT GAATTGATGG AAGATTTACA AGGTATGGGT GAGAGTAACT
301GTGCTTGGAA TAGAAAAGCC CTGCTGCATC GAGACACAAT GCTGGCAGCT GCGGCAGTGT
361ACAGAGAAAT GTACAGAAAT GAAGATGGTT CAGTACCTGC TACATACCAG ATCTATTACA
421TGATAGGATG GAAATATCAT GAGTCACAGG CAAGACCAGC TGAAAGAGGT TCCGCAACTG
481TGTCATTTGG AGAGCTAGGA AAAATAAACA ACCTTATGCC ACCGGGGAAA AAATCACAAT
541ACCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca
601acgaac

Download FASTA (ORF) (Full)