Transcript: Human XR_001754396.1

PREDICTED: Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 5 (NDUFAF5), transcript variant X4, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NDUFAF5 (79133)
Length:
5465
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001754396.1
NBCI Gene record:
NDUFAF5 (79133)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001754396.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000160192 CATCAGAAATGGATAGCTTTA pLKO.1 1130 3UTR 100% 10.800 15.120 N NDUFAF5 n/a
2 TRCN0000159852 GAAGAGGTTACATTGCACAAT pLKO.1 339 3UTR 100% 4.950 6.930 N NDUFAF5 n/a
3 TRCN0000161362 GCAGACCGTGTATATGACATA pLKO.1 281 3UTR 100% 4.950 6.930 N NDUFAF5 n/a
4 TRCN0000162496 CACTCTATGAACTTCGGTGTT pLKO.1 537 3UTR 100% 4.050 5.670 N NDUFAF5 n/a
5 TRCN0000160102 CTTTATTAAATCCCTCCTCAA pLKO.1 1512 3UTR 100% 4.050 5.670 N NDUFAF5 n/a
6 TRCN0000163287 GCCGACCAAATTTGACTACCT pLKO.1 235 3UTR 100% 2.640 3.696 N NDUFAF5 n/a
7 TRCN0000162305 CGTGTATATGACATACCCAGA pLKO.1 287 3UTR 100% 2.160 3.024 N NDUFAF5 n/a
8 TRCN0000158970 GAGGTTACATTGCACAATATT pLKO.1 342 3UTR 100% 15.000 10.500 N NDUFAF5 n/a
9 TRCN0000162482 CCTGCTACATACCAGATCTAT pLKO.1 949 3UTR 100% 5.625 3.938 N NDUFAF5 n/a
10 TRCN0000159719 GTTTCTGTTCTCATAAGGATA pLKO.1 1269 3UTR 100% 4.950 3.465 N NDUFAF5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001754396.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04052 pDONR223 100% 17.4% None 1_43del;735_832del;1093_5465del n/a
2 ccsbBroad304_04052 pLX_304 0% 17.4% V5 1_43del;735_832del;1093_5465del n/a
3 TRCN0000479874 TAGACTCACTTTTCAGATGCCCAT pLX_317 31.2% 17.4% V5 1_43del;735_832del;1093_5465del n/a
4 ccsbBroadEn_12547 pDONR223 100% 8.6% None 1_520del;735_832del;1093_5465del n/a
5 ccsbBroad304_12547 pLX_304 0% 8.6% V5 1_520del;735_832del;1093_5465del n/a
6 TRCN0000473995 CGCGATTGGAGGAAGGCCAGTAGC pLX_317 100% 8.6% V5 1_520del;735_832del;1093_5465del n/a
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