Transcript: Human XM_024451999.1

PREDICTED: Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor 5 (NDUFAF5), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NDUFAF5 (79133)
Length:
1238
CDS:
235..801

Additional Resources:

NCBI RefSeq record:
XM_024451999.1
NBCI Gene record:
NDUFAF5 (79133)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024451999.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000160192 CATCAGAAATGGATAGCTTTA pLKO.1 836 3UTR 100% 10.800 15.120 N NDUFAF5 n/a
2 TRCN0000159852 GAAGAGGTTACATTGCACAAT pLKO.1 143 5UTR 100% 4.950 6.930 N NDUFAF5 n/a
3 TRCN0000161362 GCAGACCGTGTATATGACATA pLKO.1 85 5UTR 100% 4.950 6.930 N NDUFAF5 n/a
4 TRCN0000162496 CACTCTATGAACTTCGGTGTT pLKO.1 341 CDS 100% 4.050 5.670 N NDUFAF5 n/a
5 TRCN0000160102 CTTTATTAAATCCCTCCTCAA pLKO.1 1218 3UTR 100% 4.050 5.670 N NDUFAF5 n/a
6 TRCN0000162305 CGTGTATATGACATACCCAGA pLKO.1 91 5UTR 100% 2.160 3.024 N NDUFAF5 n/a
7 TRCN0000158970 GAGGTTACATTGCACAATATT pLKO.1 146 5UTR 100% 15.000 10.500 N NDUFAF5 n/a
8 TRCN0000162482 CCTGCTACATACCAGATCTAT pLKO.1 655 CDS 100% 5.625 3.938 N NDUFAF5 n/a
9 TRCN0000159719 GTTTCTGTTCTCATAAGGATA pLKO.1 975 3UTR 100% 4.950 3.465 N NDUFAF5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024451999.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12547 pDONR223 100% 84% 84% None 1_90del n/a
2 ccsbBroad304_12547 pLX_304 0% 84% 84% V5 1_90del n/a
3 TRCN0000473995 CGCGATTGGAGGAAGGCCAGTAGC pLX_317 100% 84% 84% V5 1_90del n/a
4 ccsbBroadEn_04052 pDONR223 100% 59.3% 59.3% None 0_1ins387 n/a
5 ccsbBroad304_04052 pLX_304 0% 59.3% 59.3% V5 0_1ins387 n/a
6 TRCN0000479874 TAGACTCACTTTTCAGATGCCCAT pLX_317 31.2% 59.3% 59.3% V5 0_1ins387 n/a
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