Construct: ORF ccsbBroadEn_12725

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007532.1_s300c1, BRDN0000388888
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SNX27 (81609)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81609 SNX27 sorting nexin 27 XM_005245511.4 64% 64% 1_369del
2 human 81609 SNX27 sorting nexin 27 XM_024450039.1 64% 64% 1_369del
3 human 81609 SNX27 sorting nexin 27 XM_024450038.1 61.4% 61.1% (many diffs)
4 human 81609 SNX27 sorting nexin 27 XM_017002417.1 53.8% 53.8% 1_564del
5 human 81609 SNX27 sorting nexin 27 XM_011510025.2 51.9% 51.6% (many diffs)
6 human 81609 SNX27 sorting nexin 27 XM_005245510.3 49.8% 49.5% (many diffs)
7 human 81609 SNX27 sorting nexin 27 XM_011510024.2 49.5% 49.3% (many diffs)
8 human 81609 SNX27 sorting nexin 27 NM_030918.6 41.4% 41.4% 1_927del
9 human 81609 SNX27 sorting nexin 27 NM_001330723.2 40.3% 40.1% (many diffs)
10 human 81609 SNX27 sorting nexin 27 XM_011510026.2 17.8% 15.7% (many diffs)
11 mouse 76742 Snx27 sorting nexin family member 27 XM_006502258.3 56.8% 60% (many diffs)
12 mouse 76742 Snx27 sorting nexin family member 27 NM_029721.2 38.4% 40.8% (many diffs)
13 mouse 76742 Snx27 sorting nexin family member 27 NM_001082484.2 37.4% 39.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
723
ORF length:
657
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA CAGTACGACA GTGAATTACT TTGCCTTATT TGAAGTGATC AGTCACTCCT
121TTGTACGTAA ATTGGCACCT AATGAGTTTC CTCACAAACT CTACATTCAG AATTATACAT
181CAGCTGTGCC AGGCACCTGC TTGACCATTC GAAAGTGGCT TTTTACAACA GAAGAAGAAA
241TTCTCTTAAA TGACAATGAC CTTGCTGTTA CCTACTTCTT TCATCAGGCA GTCGATGATG
301TGAAGAAAGG TTACATCAAA GCAGAAGAAA AGTCCTATCA ATTACAGAAG CTATACGAAC
361AAAGAAAAAT GGTCATGTAC CTCAACATGC TAAGGACTTG TGAGGGCTAC AATGAAATCA
421TCTTTCCCCA CTGTGCCTGT GACTCCAGGA GGAAGGGGCA CGTTATCACA GCCATCAGCA
481TCACGCACTT TAAACTGCAT GCCTGCACTG AAGAAGGACA GCTGGAGAAC CAGGTAATTG
541CATTTGAATG GGATGAGATG CAGCGATGGG ACACAGATGA AGAAGGGATG GCCTTCTGTT
601TCGAATATGC ACGAGGAGAG AAGAAGCCCC GATGGGTTAA AATCTTCACG CCATATTTCA
661ATTACATGCA TGAGTGCTTC GAGAGGGTGT TCTGCGAGCT CAAGTGGAGA AAAGAGGAAT
721ATTACCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt
781gcaacgaac

Download FASTA (ORF) (Full)