Transcript: Mouse NM_029721.2

Mus musculus sorting nexin family member 27 (Snx27), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Snx27 (76742)
Length:
6273
CDS:
168..1748

Additional Resources:

NCBI RefSeq record:
NM_029721.2
NBCI Gene record:
Snx27 (76742)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_029721.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000245095 CAAATTAGCTGCACGTATATA pLKO_005 2974 3UTR 100% 15.000 21.000 N SNX27 n/a
2 TRCN0000253472 TGCACTGTTCAAGCATATATA pLKO_005 2922 3UTR 100% 15.000 21.000 N Snx27 n/a
3 TRCN0000253475 ATTTGAAGTGATCAATCATTC pLKO_005 1121 CDS 100% 10.800 15.120 N Snx27 n/a
4 TRCN0000253473 AGCTGGAGAACCAGGTAATAG pLKO_005 1543 CDS 100% 13.200 9.240 N Snx27 n/a
5 TRCN0000245094 ATTAGTTAGAGACTGATTATC pLKO_005 1744 CDS 100% 13.200 9.240 N SNX27 n/a
6 TRCN0000253474 CCTAATGAATTTCCTCATAAA pLKO_005 1161 CDS 100% 13.200 9.240 N Snx27 n/a
7 TRCN0000265386 CTGCGAGGGCTACAATGAAAT pLKO_005 1421 CDS 100% 13.200 9.240 N Snx27 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_029721.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12725 pDONR223 100% 38.4% 40.8% None (many diffs) n/a
2 ccsbBroad304_12725 pLX_304 0% 38.4% 40.8% V5 (many diffs) n/a
3 TRCN0000478297 CCAGACCGGAACGTTATTTTCAGG pLX_317 46.2% 38.4% 40.8% V5 (many diffs) n/a
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