Construct: ORF ccsbBroadEn_13168
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017528.1_s300c1, BRDN0000397303
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL32P3 (132241)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6161 | RPL32 | ribosomal protein L32 | NM_000994.4 | 23.4% | 23.7% | (many diffs) |
| 2 | human | 6161 | RPL32 | ribosomal protein L32 | NM_001007073.1 | 23.4% | 23.7% | (many diffs) |
| 3 | human | 6161 | RPL32 | ribosomal protein L32 | NM_001007074.1 | 23.4% | 23.7% | (many diffs) |
| 4 | human | 338699 | ANKRD42 | ankyrin repeat domain 42 | NM_001300976.2 | 9.5% | 8.4% | (many diffs) |
| 5 | human | 132241 | RPL32P3 | ribosomal protein L32 pseud... | NR_003111.2 | 4.6% | 1_437del;534_534delAinsCG;536_2079del | |
| 6 | human | 338699 | ANKRD42 | ankyrin repeat domain 42 | NR_125355.2 | 2.5% | (many diffs) | |
| 7 | mouse | 621697 | Rpl32l | ribosomal protein L32-like | NM_001101561.2 | 21.8% | 24% | (many diffs) |
| 8 | mouse | 19951 | Rpl32 | ribosomal protein L32 | NM_172086.2 | 21.7% | 23.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 165
- ORF length:
- 99
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC TGCCCTCAGA TCCCTTGTGA AGCCCAAGAT CGTCAAAAAG AGAACCAAGA 121 AATTCATCCG GCACCAGTCA GACCGATATG TCAAAATCAA GCGTTACCCA ACTTTCTTGT 181 ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c