Construct: ORF ccsbBroadEn_13290

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017383.1_s300c1, BRDN0000394741
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RASGEF1B (153020)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 153020 RASGEF1B RasGEF domain family member 1B NM_001300735.2 100% 100%
2 human 153020 RASGEF1B RasGEF domain family member 1B NM_152545.3 99.7% 99.7% 437_439delAGC
3 human 153020 RASGEF1B RasGEF domain family member 1B XM_017007813.1 99.7% 99.7% 437_439delAGC
4 human 153020 RASGEF1B RasGEF domain family member 1B NM_001300736.2 91.3% 91.3% 177_178ins123
5 human 153020 RASGEF1B RasGEF domain family member 1B XM_017007812.1 91.1% 91.1% 177_178ins123;314_316delAGC
6 mouse 320292 Rasgef1b RasGEF domain family, membe... NM_181318.4 87.2% 97.4% (many diffs)
7 mouse 320292 Rasgef1b RasGEF domain family, membe... NM_145839.2 87.1% 97.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1485
ORF length:
1416
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GCCTCAGACT CCTCCCTTTT CAGCAATGTT TGACAGCAGT GGTTACAATC
121GAAACCTCTA TCAGTCTGCA GAGGACAGCT GTGGAGGGTT GTATTACCAT GACAACAACC
181TCCTCTCTGG ATCCCTGGAA GCACTCATCC AGCACTTAGT ACCTAATGTG GATTACTATC
241CAGATAGAAC ATACATATTT ACCTTCCTAC TCAGTTCTCG GTTATTTATG CATCCGTATG
301AGCTAATGGC CAAAGTTTGC CACTTATGTG TTGAGCACCA GAGACTAAGT GATCCTGATA
361GTGATAAGAA CCAGATGAGA AAAATTGCAC CCAAAATCCT TCAACTCCTC ACGGAATGGA
421CGGAAACATT TCCCTATGAT TTTCGGGATG AAAGAATGAT GAGAAACTTA AAAGATCTGG
481CTCACCGAAT AGCCAGTGGC GAAGAGACAT ACAGAAAGAA TGTCCAGCAA ATGATGCAGT
541GTCTGATCCG CAAGCTTGCT GCGCTCAGCC AGTACGAAGA AGTCCTGGCA AAAATCAGCT
601CCACATCCAC AGATCGGCTC ACAGTTCTCA AGACCAAGCC ACAGTCTATA CAAAGGGATA
661TCATTACTGT CTGCAACGAC CCTTACACGT TGGCCCAGCA GCTGACTCAT ATAGAGCTGG
721AGAGGCTCAA TTATATTGGG CCAGAAGAAT TTGTTCAGGC GTTCGTGCAG AAGGACCCTT
781TGGATAATGA CAAGAGTTGC TACAGTGAAC GGAAGAAAAC ACGAAACTTA GAAGCTTACG
841TGGAATGGTT TAATCGCCTC AGCTACTTGG TTGCTACAGA AATCTGTATG CCTGTTAAGA
901AAAAACACCG AGCAAGAATG ATTGAGTATT TCATTGACGT AGCTCGGGAG TGTTTTAACA
961TTGGCAACTT CAACTCCTTG ATGGCGATAA TCTCTGGTAT GAATATGAGC CCAGTCTCTC
1021GACTAAAAAA AACTTGGGCC AAAGTGAAGA CTGCAAAATT TGACATTCTT GAGCATCAGA
1081TGGACCCTTC AAGCAATTTC TATAATTATC GAACAGCTCT TCGTGGGGCA GCACAAAGGT
1141CTTTAACTGC TCATAGTAGT AGAGAAAAGA TTGTGATACC ATTCTTCAGT CTCTTAATCA
1201AAGATATTTA TTTCCTCAAT GAGGGTTGTG CCAACCGCCT TCCCAATGGC CATGTCAATT
1261TTGAGAAATT TTGGGAACTG GCCAAACAAG TGAGTGAATT TATGACATGG AAACAAGTGG
1321AGTGTCCATT TGAGAGGGAC CGGAAGATCT TGCAGTATCT GCTCACAGTA CCAGTCTTCA
1381GTGAAGATGC TCTCTACTTG GCTTCTTATG AGAGTGAAGG ACCTGAAAAT CATATAGAGA
1441AAGACAGATG GAAGTCTTTA AGGTCGAGCC TCTTAGGCAG AGTTTTGCCA ACTTTCTTGT
1501ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)