Transcript: Human NM_001300736.2

Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
RASGEF1B (153020)
Length:
2815
CDS:
145..1440

Additional Resources:

NCBI RefSeq record:
NM_001300736.2
NBCI Gene record:
RASGEF1B (153020)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001300736.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000077695 GCCAAACAAGTGAGTGAATTT pLKO.1 1234 CDS 100% 13.200 9.240 N Rasgef1b n/a
2 TRCN0000072966 GCTCTCTACTTGGCTTCTTAT pLKO.1 1342 CDS 100% 13.200 9.240 N RASGEF1B n/a
3 TRCN0000072964 CGGAAACATTTCCCTATGATT pLKO.1 374 CDS 100% 5.625 3.938 N RASGEF1B n/a
4 TRCN0000072967 GAAGCACTCATCCAGCACTTA pLKO.1 274 CDS 100% 4.950 3.465 N RASGEF1B n/a
5 TRCN0000072963 GCTGACAGATAGACTCAGATT pLKO.1 1620 3UTR 100% 4.950 2.970 N RASGEF1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001300736.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13290 pDONR223 100% 91.3% 91.3% None 177_178ins123 n/a
2 ccsbBroad304_13290 pLX_304 0% 91.3% 91.3% V5 177_178ins123 n/a
3 TRCN0000467494 GTTCTTGGTGCAGCTCGATGCTAT pLX_317 30.5% 91.3% 91.3% V5 177_178ins123 n/a
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