Construct: ORF ccsbBroadEn_13807

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002420.1_s300c1, BRDN0000397003
DNA Barcode:
None
Epitope Tag:
None
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
ACTA1 (58)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 58 ACTA1 actin alpha 1, skeletal muscle NM_001100.4 99.9% 1.8% 4delT
2 human 60 ACTB actin beta NM_001101.5 85.8% 1.8% (many diffs)
3 human 71 ACTG1 actin gamma 1 NM_001199954.2 85.8% 1.8% (many diffs)
4 human 71 ACTG1 actin gamma 1 NM_001614.5 85.8% 1.8% (many diffs)
5 human 70 ACTC1 actin alpha cardiac muscle 1 NM_005159.5 85.5% 1.8% (many diffs)
6 human 71 ACTG1 actin gamma 1 NR_037688.3 54.6% (many diffs)
7 mouse 11459 Acta1 actin, alpha 1, skeletal mu... NM_001272041.1 90.8% 1.8% (many diffs)
8 mouse 11459 Acta1 actin, alpha 1, skeletal mu... NM_009606.3 90.8% 1.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
120
ORF length:
54
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TCTGCATGGC GACGAAGACG AGACCACCGC CCTCGTGTGC GACAATGGCT CCGGCCTGGT
121GAAAGCCGGC TTCGCCGGGG ATGACGCCCC TAGGGCCGTG TTCCCGTCCA TCGTGGGCCG
181CCCCCGACAC CAGGGCGTCA TGGTCGGTAT GGGTCAGAAA GATTCCTACG TGGGCGACGA
241GGCTCAGAGC AAGAGAGGTA TCCTGACCCT GAAGTACCCT ATCGAGCACG GCATCATCAC
301CAACTGGGAT GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TTCGCGTGGC
361TCCCGAGGAG CACCCCACCC TGCTCACCGA GGCCCCCCTC AATCCCAAGG CCAACCGCGA
421GAAGATGACC CAGATCATGT TTGAGACCTT CAACGTGCCC GCCATGTACG TGGCCATCCA
481GGCCGTGCTG TCCCTCTACG CCTCCGGCAG GACCACCGGC ATCGTGCTGG ACTCCGGCGA
541CGGCGTCACC CACAACGTGC CCATTTATGA GGGCTACGCG CTGCCGCACG CCATCATGCG
601CCTGGACCTG GCGGGCCGCG ATCTCACCGA CTACCTGATG AAGATCCTCA CTGAGCGTGG
661CTACTCCTTC GTGACCACAG CTGAGCGCGA GATCGTGCGC GACATCAAGG AGAAGCTGTG
721CTACGTGGCC CTGGACTTCG AGAACGAGAT GGCGACGGCC GCCTCCTCCT CCTCCCTGGA
781AAAGAGCTAC GAGCTGCCAG ACGGGCAGGT CATCACCATC GGCAACGAGC GCTTCCGCTG
841CCCGGAGACG CTCTTCCAGC CCTCCTTCAT CGGTATGGAG TCGGCGGGCA TTCACGAGAC
901CACCTACAAC AGCATCATGA AGTGTGACAT CGACATCAGG AAGGACCTGT ATGCCAACAA
961CGTCATGTCG GGGGGCACCA CGATGTACCC TGGGATCGCT GACCGCATGC AGAAAGAGAT
1021CACCGCGCTG GCACCCAGCA CCATGAAGAT CAAGATCATC GCCCCGCCGG AGCGCAAATA
1081CTCGGTGTGG ATCGGCGGCT CCATCCTGGC CTCGCTGTCC ACCTTCCAGC AGATGTGGAT
1141CACCAAGCAG GAGTACGACG AGGCCGGCCC TTCCATCGTC CACCGCAAAT GCTTCTACCC
1201AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt gttgcaacga
1261ac

Download FASTA (ORF) (Full)