Transcript: Human NM_001199954.2

Homo sapiens actin gamma 1 (ACTG1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-26
Taxon:
Homo sapiens (human)
Gene:
ACTG1 (71)
Length:
2056
CDS:
192..1319

Additional Resources:

NCBI RefSeq record:
NM_001199954.2
NBCI Gene record:
ACTG1 (71)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001199954.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000029474 GCTGGCAAGAACCAGTTGTTT pLKO.1 1724 3UTR 100% 5.625 7.875 N ACTG1 n/a
2 TRCN0000293291 GCTGGCAAGAACCAGTTGTTT pLKO_005 1724 3UTR 100% 5.625 7.875 N ACTG1 n/a
3 TRCN0000293359 TCTAAACGGACTCAGCAGATG pLKO_005 1315 CDS 100% 4.050 3.240 N ACTG1 n/a
4 TRCN0000029475 CCGAGCCGTGTTTCCTTCCAT pLKO.1 272 CDS 100% 1.000 0.700 N ACTG1 n/a
5 TRCN0000293290 CCGAGCCGTGTTTCCTTCCAT pLKO_005 272 CDS 100% 1.000 0.700 N ACTG1 n/a
6 TRCN0000029478 CGCATCCTCCTCTTCTCTGGA pLKO.1 881 CDS 100% 0.880 0.616 N ACTG1 n/a
7 TRCN0000029477 GCGTGGCATCCTGACCCTGAA pLKO.1 374 CDS 100% 0.000 0.000 N ACTG1 n/a
8 TRCN0000293292 GGCATTGTCATGGACTCTGGA pLKO_005 639 CDS 100% 2.640 1.584 N ACTG1 n/a
9 TRCN0000029476 CCAGCAGATGTGGATTAGCAA pLKO.1 1247 CDS 100% 3.000 1.500 Y ACTG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001199954.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15351 pDONR223 0% 99.9% 100% None 918C>T n/a
2 ccsbBroad304_15351 pLX_304 0% 99.9% 100% V5 918C>T n/a
3 ccsbBroadEn_13808 pDONR223 100% 99.9% 99.4% None 1121delG n/a
4 ccsbBroad304_13808 pLX_304 0% 99.9% 99.4% V5 (not translated due to frame shift) 1121delG n/a
5 TRCN0000471742 AATGGGAGTTCTCACGTCAGGTCC pLX_317 44.8% 99.9% 99.4% V5 (not translated due to frame shift) 1121delG n/a
6 ccsbBroadEn_05764 pDONR223 100% 99.8% 100% None 918C>T;930C>T n/a
7 ccsbBroad304_05764 pLX_304 0% 99.8% 100% V5 918C>T;930C>T n/a
8 TRCN0000466781 AATTGTGGTGCTTGTTGCCGCCTG pLX_317 31.7% 99.8% 100% V5 918C>T;930C>T n/a
9 ccsbBroadEn_05763 pDONR223 100% 91.2% 98.6% None (many diffs) n/a
10 ccsbBroad304_05763 pLX_304 53.1% 91.2% 98.6% V5 (many diffs) n/a
11 TRCN0000470279 TCAAGCCTGGAACGGGCTCACGTC pLX_317 31.7% 91.2% 98.6% V5 (many diffs) n/a
12 ccsbBroadEn_13807 pDONR223 100% 85.8% 1.8% None (many diffs) n/a
13 ccsbBroad304_13807 pLX_304 0% 85.8% 1.8% V5 (not translated due to prior stop codon) (many diffs) n/a
14 TRCN0000475388 CAGGCCGACAGAGACACGAAATCC pLX_317 29.9% 85.8% 1.8% V5 (not translated due to prior stop codon) (many diffs) n/a
15 ccsbBroadEn_00015 pDONR223 100% 83% 93.6% None (many diffs) n/a
16 ccsbBroad304_00015 pLX_304 0% 83% 93.6% V5 (many diffs) n/a
17 TRCN0000468012 TCTATATTCTTCCCTCACTCATAA pLX_317 37.9% 83% 93.6% V5 (many diffs) n/a
18 ccsbBroadEn_00014 pDONR223 100% 82.3% 93.6% None (many diffs) n/a
19 ccsbBroad304_00014 pLX_304 0% 82.3% 93.6% V5 (not translated due to prior stop codon) (many diffs) n/a
20 TRCN0000467679 GCTACTGCTTTGAGGGTTATAACT pLX_317 37.9% 82.3% 93.6% V5 (not translated due to prior stop codon) (many diffs) n/a
21 ccsbBroadEn_05497 pDONR223 100% 80.9% 90.9% None (many diffs) n/a
22 ccsbBroad304_05497 pLX_304 0% 80.9% 90.9% V5 (many diffs) n/a
23 TRCN0000480996 TCGCGGAGACTCACCCAATCCCGC pLX_317 35.4% 80.9% 90.9% V5 (many diffs) n/a
24 ccsbBroadEn_14514 pDONR223 100% 50% 45% None (many diffs) n/a
25 ccsbBroad304_14514 pLX_304 0% 50% 45% V5 (not translated due to frame shift) (many diffs) n/a
26 TRCN0000481436 GCTCGTTTACTAGTATTTTTAATC pLX_317 64.2% 50% 45% V5 (not translated due to frame shift) (many diffs) n/a
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