Construct: ORF ccsbBroadEn_14561

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015777.2_s300c1, BRDN0000458543
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CAMK4 (814)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 814 CAMK4 calcium/calmodulin dependen... NM_001323374.2 99.9% 100% 1011G>C
2 human 814 CAMK4 calcium/calmodulin dependen... NM_001323375.2 99.9% 100% 1011G>C
3 human 814 CAMK4 calcium/calmodulin dependen... NM_001744.6 99.9% 100% 1011G>C
4 human 814 CAMK4 calcium/calmodulin dependen... NM_001323376.2 58.2% 58.3% 0_1ins591;420G>C
5 human 814 CAMK4 calcium/calmodulin dependen... NM_001323377.2 58.2% 58.3% 0_1ins591;420G>C
6 human 814 CAMK4 calcium/calmodulin dependen... XR_001742282.2 25.4% 1_100del;1111G>C;1520_5562del
7 human 814 CAMK4 calcium/calmodulin dependen... XR_001742281.2 24.9% 1_100del;1111G>C;1520_5681del
8 human 814 CAMK4 calcium/calmodulin dependen... XR_948303.2 13.5% 1_100del;1111G>C;1520_10502del
9 mouse 12326 Camk4 calcium/calmodulin-dependen... NM_009793.3 82.9% 80.6% (many diffs)
10 mouse 12326 Camk4 calcium/calmodulin-dependen... XM_006525544.3 82.9% 80.6% (many diffs)
11 mouse 12326 Camk4 calcium/calmodulin-dependen... XM_006525546.3 82.9% 80.6% (many diffs)
12 mouse 12326 Camk4 calcium/calmodulin-dependen... XM_006525543.2 78.4% 76.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1485
ORF length:
1419
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgct caaagtcacg gtgccctcct gctccgcctc gtcctgctct tcggtcaccg
121ccagtgcggc cccggggacc gcgagcctcg tcccggatta ctggatcgac ggctccaaca
181gggatgcgct gagcgatttc ttcgaggtgg agtcggagct gggacggggt gctacatcca
241ttgtgtacag atgcaaacag aaggggaccc agaagcctta tgctctcaaa gtgttaaaga
301aaacagtgga caaaaaaatc gtaagaactg agataggagt tcttcttcgc ctctcacatc
361caaacattat aaaacttaaa gagatatttg aaacccctac agaaatcagt ctggtcctag
421aactcgtcac aggaggagaa ctgtttgata ggattgtgga aaagggatat tacagtgagc
481gagatgctgc agatgccgtt aaacaaatcc tggaggcagt tgcttatcta catgaaaatg
541ggattgtcca tcgtgatctc aaaccagaga atcttcttta tgcaactcca gccccagatg
601caccactcaa aatcgctgat tttggactct ctaaaattgt ggaacatcaa gtgctcatga
661agacagtatg tggaacccca gggtactgcg cacctgaaat tcttagaggt tgtgcctatg
721gacctgaggt ggacatgtgg tctgtaggaa taatcaccta catcttactt tgtggatttg
781aaccattcta tgatgaaaga ggcgatcagt tcatgttcag gagaattctg aattgtgaat
841attactttat ctccccctgg tgggatgaag tatctctaaa tgccaaggac ttggtcagaa
901aattaattgt tttggatcca aagaaacggc tgactacatt tcaagctctc cagcatccgt
961gggtcacagg taaagcagcc aattttgtac acatggatac cgctcaaaag aagctccaag
1021aattcaatgc ccggcgtaag cttaaggcag cggtgaaggc tgtggtggcc tcttcccgcc
1081tgggaagtgc cagcagcagc catggcagca tccaggagag ccacaaggct agccgagacc
1141cttctccaat ccaagatggc aacgaggaca tgaaagctat tccagaagga gagaaaattc
1201aaggcgatgg ggcccaagcc gcagttaagg gggcacaggc tgagctgatg aaggtgcaag
1261ccttagagaa agttaaaggt gcagatataa atgctgaaga ggcccccaaa atggtgccca
1321aggcagtgga ggatgggata aaggtggctg acctggaact agaggagggc ctagcagagg
1381agaagctgaa gactgtggag gaggcagcag ctcccagaga agggcaagga agctctgctg
1441tgggttttga agttccacag caagatgtga tcctgccaga gtactaccca actttcttgt
1501acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)