Transcript: Human XR_001742282.2

PREDICTED: Homo sapiens calcium/calmodulin dependent protein kinase IV (CAMK4), transcript variant X3, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CAMK4 (814)
Length:
5562
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001742282.2
NBCI Gene record:
CAMK4 (814)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001742282.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000226390 TGATCCTGCCAGAGTACTAAA pLKO_005 1503 3UTR 100% 13.200 18.480 N CAMK4 n/a
2 TRCN0000009964 CAAAGAAACGGCTGACTACAT pLKO.1 954 3UTR 100% 4.950 6.930 N CAMK4 n/a
3 TRCN0000009960 TCGTAAGAACTGAGATAGGAG pLKO.1 354 3UTR 100% 2.640 3.696 N CAMK4 n/a
4 TRCN0000009963 GAGGCGATCAGTTCATGTTCA pLKO.1 834 3UTR 100% 4.950 3.960 N CAMK4 n/a
5 TRCN0000217998 CATGTGGTCTGTAGGAATAAT pLKO_005 769 3UTR 100% 15.000 10.500 N CAMK4 n/a
6 TRCN0000257407 ATGCTGCAGATGCCGTTAAAC pLKO_005 519 3UTR 100% 13.200 9.240 N CAMK4 n/a
7 TRCN0000199982 GCAGTGGAGGATGGGATAAAG pLKO.1 1358 3UTR 100% 13.200 9.240 N CAMK4 n/a
8 TRCN0000000578 GCCTCTCACATCCAAACATTA pLKO.1 384 3UTR 100% 13.200 9.240 N CAMK4 n/a
9 TRCN0000226389 ATCCGTGGGTCACAGGTAAAG pLKO_005 990 3UTR 100% 10.800 7.560 N CAMK4 n/a
10 TRCN0000196662 GAGAACTGTTTGATAGGATTG pLKO.1 471 3UTR 100% 6.000 4.200 N CAMK4 n/a
11 TRCN0000000579 AGAAAGTTAAAGGTGCAGATA pLKO.1 1302 3UTR 100% 4.950 3.465 N CAMK4 n/a
12 TRCN0000000581 CAACGAGGACATGAAAGCTAT pLKO.1 1195 3UTR 100% 4.950 3.465 N CAMK4 n/a
13 TRCN0000009962 GATATTACAGTGAGCGAGATG pLKO.1 501 3UTR 100% 4.050 2.835 N CAMK4 n/a
14 TRCN0000000580 GTCACAGGTAAAGCAGCCAAT pLKO.1 998 3UTR 100% 4.050 2.835 N CAMK4 n/a
15 TRCN0000009961 TGGTCCTAGAACTCGTCACAG pLKO.1 447 3UTR 100% 4.050 2.835 N CAMK4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001742282.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000480036 CACTTAGCGCAATCTTGCATAACG pLX_317 24.6% 25.4% V5 1_100del;1111G>C;1520_5562del n/a
2 ccsbBroadEn_05929 pDONR223 100% 25.4% None (many diffs) n/a
3 ccsbBroad304_05929 pLX_304 0% 25.4% V5 (many diffs) n/a
4 ccsbBroadEn_14561 pDONR223 0% 25.4% None 1_100del;1111G>C;1520_5562del n/a
5 ccsbBroad304_14561 pLX_304 0% 25.4% V5 1_100del;1111G>C;1520_5562del n/a
6 TRCN0000480966 CAAAGATTTTAGCGCCCGCTTTCG pLX_317 29.4% 25.4% V5 (not translated due to frame shift) (many diffs) n/a
7 TRCN0000488719 GAACGTGCGGTGTATCGCACCCCG pLX_317 19.9% 25.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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