Construct: ORF ccsbBroadEn_14609
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018916.1_s300c1, BRDN0000458212
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DAPK1 (1612)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1612 | DAPK1 | death associated protein ki... | NM_001288729.1 | 99.3% | 15.8% | (many diffs) |
| 2 | human | 1612 | DAPK1 | death associated protein ki... | NM_001288730.2 | 99.3% | 15.8% | (many diffs) |
| 3 | human | 1612 | DAPK1 | death associated protein ki... | NM_001288731.2 | 99.3% | 15.8% | (many diffs) |
| 4 | human | 1612 | DAPK1 | death associated protein ki... | NM_004938.4 | 99.3% | 15.8% | (many diffs) |
| 5 | human | 1612 | DAPK1 | death associated protein ki... | XM_017014360.1 | 69.9% | 5.2% | (many diffs) |
| 6 | human | 1612 | DAPK1 | death associated protein ki... | XM_005251757.4 | 57.5% | 26.8% | (many diffs) |
| 7 | mouse | 69635 | Dapk1 | death associated protein ki... | NM_029653.3 | 87.1% | 15.8% | (many diffs) |
| 8 | mouse | 69635 | Dapk1 | death associated protein ki... | NM_134062.2 | 87.1% | 15.8% | (many diffs) |
| 9 | mouse | 69635 | Dapk1 | death associated protein ki... | NM_001285917.1 | 86.4% | 15.6% | (many diffs) |
| 10 | mouse | 69635 | Dapk1 | death associated protein ki... | XM_006517370.3 | 86.4% | 15.6% | (many diffs) |
| 11 | mouse | 69635 | Dapk1 | death associated protein ki... | XM_006517371.3 | 85.9% | 14.4% | (many diffs) |
| 12 | mouse | 69635 | Dapk1 | death associated protein ki... | XM_017315598.1 | 85.1% | 14.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 81
- ORF end:
- 786
- ORF length:
- 705
- Sequence:
-
1 CGATAGTGAC CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT 61 TGTACAAAAA AGTTGGCACC ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG 121 ACACCGGCGA GGAACTTGGC AGTGGACAGT TTGCGGTTGT GAAGAAATGC CGTGAGAAAA 181 GCACCGGCCT CCAGTATGCC GCCAAATTCA TCAAGAAAAG GAGGACTAAG TCCAGCCGGC 241 GGGGTGTGAG CCGCGAGGAC ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC 301 CCAATGTCAT CACCCTGCAC GAGGTCTATG AGAACAAGAC GGACGTCATC CTGATCTTGG 361 AACTCGTTGC AGGTGGCGAG CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTAACTGAAG 421 AGGAAGCAAC TGAATTTCTC AAACAAATTC TTAATGGTGT TTACTACCTG CACTCCCTTC 481 AAATCGCCCA CTTTGATCTT AAGCCTGAGA ACATAATGCT TTTGGATAGA AATGTCCCCA 541 AACCTCGGAT CAAGATCATT GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT 601 TTAAAAACAT ATTTGGGACT CCAGAGTTTG TCGCTCCTGA GATAGTCAAC TATGAACCTC 661 TTGGTCTTGA GGCAGATATG TGGAGTATCG GGGTAATAAC CTATATCCTC CTAAGTGGGG 721 CCTCCCCATT TCTTGGAGAC ACTAAGCAAG AAACGTTAGC AATGTATCCG CTGTCAACTA 781 CGATTTGAGA TGATACTTCA GTATACCAGT GCCCTAGCCA AAGATTTCAT AGAGACTTCT 841 GGTCAGATCC AAAGAGAGAT GACATTCAGA TAGTTGCAgc atccctggat caagcctaaa 901 gatacacaac aggcacttag tagaaaagca tcagcagtaa acatggagaa attcaagaag 961 tttgcagccc ggaaaaaatg gaaacaatcc gttcgcttga tatcactgtg ccaaagatta 1021 tccaggtcat tcctgtccag aagtaacatg agtgttgcca gaagcgatga tactctggat 1081 gaggaagact cctttgtgat gaaagccatc atccatgcca tcaacgatga caatgtccca 1141 ggcctgcagc accttctggg ctcattatcc aactatgatg ttaaccaacc caacaagcac 1201 gggacacctc cattactcat tgctgctggc tgtgggaata ttcaaatact acagttgctc 1261 attaaaagag gctcgagaat cgatgtccag gataagggcg ggtccaatgc cgtctactgg 1321 gctgctcggc atggccacgt cgataccttg aaatttctca gtgagaacaa atgccctttg 1381 gatgtgaaag acaagtctgg agagatggcc ctccacgtgg cagctcgcta tggccatgct 1441 gacgtggctc agttactgtg cagcttcggc tcaaatccca atatccagga caaggaagaa 1501 gaaacccccc tgcactgtgc tgcttggcac ggctattact ctgtggccaa agccctttgt 1561 gaagccggct gtaacgtgaa catcaagaac cgagaaggag agacgcccct cctgacagcc 1621 tctgccaggg gctaccacga catcgtggag tgtctggccg aacatggagc cgaccttaat 1681 gcttgcgaca aggacggaca cattgccctt catctggctg taagacggtg tcagatggag 1741 gtaatcaaga ctctcctcag ccaagggtgt ttcgtcgatt atcaagacag gcacggcaat 1801 actcccctcc atgtggcatg taaagatggc aacatgccta tcgtggtggc cctctgtgaa 1861 gcaaactgca atttggacat ctccaacaag tatgggcgaa cgcctctgca ccttgcggcc 1921 aacaacggaa tcctagacgt ggtccggtat ctctgtctga tgggagccag cgttgaggcg 1981 ctgaccacgg acggaaagac ggcagaagat cttgctagat cggaacagca cgagcacgta 2041 gcaggtctcc ttgcaagact tcgaaaggat acgcaccgag gactcttcat ccagcagctc 2101 cgacccacac agaacctgca gccaagaatt aagctcaagc tgtttggcca ctcgggatcc 2161 gggaaaacca cccttgtaga atctctcaag tgtgggctgc tgaggagctt tttcagaagg 2221 cgtcggccca gactgtcttc caccaactcc agcaggttcc caccttcacc cctggcttct 2281 aagcccacag tctcagtgag catcaacaac ctgtacccag gctgcgagaa cgtgagtgtg 2341 aggagccgca gcatgatgtt cgagccgggt cttaccaaag ggatgctgga ggtgtttgtg 2401 gccccgaccc accacccgca ctgctcggcc gatgaccagt ccaccaaggc catcgacatc 2461 cagaacgctt atttgaatgg agttggcgat ttcagcgtgt gggagttctc tggaaatcct 2521 gtgtatttct gctgttatga ctattttgct gcaaatgatc ccacgtcaat ccatgttgtt 2581 gtctttagtc tagaagagcc ctatgagatc cagctgaacc aagtgatttt ctggctcagt 2641 ttcctgaagt cccttgtccc agttgaagaa cccatagcct tcggtggcaa gctgaagaac 2701 ccactccaag ttgtcctggt ggccacccac gctgacatca tgaatgttcc tcgaccggct 2761 ggaggcgagt ttggatatga caaagacaca tcgttgctga aagagattag gaacaggttt 2821 ggaaatgatc ttcacatttc aaataagctg tttgttctgg atgctggggc ttctgggtca 2881 aaggacatga aggtacttcg aaatcatctg caagaaatac gaagccagat tgtttcggtc 2941 tgtcctccca tgactcacct gtgtgagaaa atcatctcca cgctgccttc ctggaggaag 3001 ctcaatggac ccaaccagct gatgtcgctg cagcagtttg tgtacgacgt gcaggaccag 3061 ctgaaccccc tggccagcga ggaggacctc aggcgcattg ctcagcagct ccacagcaca 3121 ggcgagatca acatcatgca aagtgaaaca gttcaggacg tgctgctcct ggacccccgc 3181 tggctctgca caaacgtcct ggggaagttg ctgtccgtgg agaccccacg ggcgctgcac 3241 cactaccggg gccgctacac cgtggaggac atccagcgcc tggtgcccga cagcgacgtg 3301 gaggagctgc tgcagatcct cgatgccatg gacatctgcg cccgggacct gagcagcggg 3361 accatggtgg acgtcccagc cctgatcaag acagacaacc tgcaccgctc ctgggctgat 3421 gaggaggacg aggtgatggt gtatggtggc gtgcgcatcg tgcccgtgga acacctcacc 3481 cccttcccat gtggcatctt tcacaaggtc caggtgaacc tgtgccggtg gatccaCCAG 3541 CAAAGCCACA GAGGCGGACG CGGACATCCC GCTTGTTGGT GAATGGTTGC AAGCTGCCCA 3601 ACGTGGGGGC CGAGCTGCTG GTGCTGCTGG TCAAACACGC CCAAGGCATT GAGGTCCAGG 3661 TCCGCGGCCT GGAGACGGAG AAGATCAAGT GCTGCCTGCT GCTGGACTCG GTGTGCAGCA 3721 CCATTGAGAA CGTCATGGCC ACCACGCTGC CAGGGCTCCT GACCGTGAAG CATTACCTGA 3781 GCCCCCAGCA GCTGCGGGAG CACCATGAGC CCGTCATGAT CTACCAGCCA CGGGACTTCT 3841 TCCGGGCACA GACTCTGAAG GAAACCTCAC TGACCAACAC CATGGGGGGG TACAAGGAAA 3901 GCTTCAGCAG CATCATGTGC TTCGGGTGTC ACGACGTCTA CTCACAGGCC AGCCTCGGCA 3961 TGGACATCCA TGCATCAGAC CTGAACCTCC TCACTCGGAG GAAACTGAGT CGCCTGCTGG 4021 ACCCGCCCGA CCCCCTGGGG AAGGACTGGT GCCTTCTCGC CATGAACTTA GGCCTCCCTG 4081 ACCTCGTGGC AAAGTACAAC ACCAGTAACG GGGCTCCCAA GGATTTCCTC CCCAGCCCCC 4141 TCCACGCCCT GCTGCGGGAA TGGACCACCT ACCCTGAGAG CACAGTGGGC ACCCTCATGT 4201 CCAAACTGAG GGAGCTGGGT CGCCGGGATG CCGCAGACTT TTTGCTGAAG GCATCCTCTG 4261 TGTTCAAAAT CAACCTGGAT GGCAATGGCC AGGAGGCCTA TGCCTCGAGC TGCAACAGCG 4321 GCACCTCTTA CAATTCCATT AGCTCTGTTG TATCCCGGTT GCCAACTTTC TTGTACAAAG 4381 TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC 4441 AAAATAAAAT CATTATTGCC ATCCAGCCTA AGCCTCCCCT G